1.The entry of Epstein-Barr virus into B lymphocytes and epithelial cells during infection.
Lie-Lian ZUO ; Mei-Juan ZHU ; Shu-Juan DU ; Jian-Hong LU ; Gui-Yuan LI
Chinese Journal of Virology 2014;30(4):476-482
Epstein-Barr virus (EBV) is a human herpesvirus associated with important human diseases, including infectious mononucleosis syndrome, malignant lymphoma, and nasopharyngeal carcinoma. The mechanism of EBV entry into host cells remains a subject of intensive research. After decades of study, researchers have identified several key proteins and different patterns of EBV intrusion into host cells. The viral surface glycoproteins, gp350/220, gp42, gB, gH, and gL, are involved in interactions with the CR2 receptor on the surface of B lymphocytes during viral entry. However, the majority of epithelial cells lack CR2 receptor expression, which makes viral invasion much more complex than in B lymphocytes. Three different models have been proposed to explain how EBV enters epithelial cells: (1) "transfer of infection", mediated by B lymphocytes or Langerhans cells; (2) EBV utilizes its own proteins during the process of fusion with the cell membrane; and (3) progeny virions arising from EBV-infected epithelial cells cross lateral membranes into adjacent epithelial cells. This review will discuss the relevant mechanism of viral entry into B lymphocytes and epithelial cells during EBV infection.
Animals
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B-Lymphocytes
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virology
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Epithelial Cells
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virology
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Epstein-Barr Virus Infections
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virology
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Herpesvirus 4, Human
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genetics
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physiology
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Humans
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Viral Proteins
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genetics
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metabolism
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Virus Internalization
2.Relationship between the level of serum E-selectin and SER128ARG polymorphisms of E-selectin in patients with acute myocardial infarction
Ming WEI ; Hong-Chun SHE ; Shu-Juan LI ; Yong-Shen LU ;
Chinese Journal of Emergency Medicine 2006;0(12):-
Objective To explore the relationship between the serum level of E-selectin and S128R polymorphisms in the exon 4 of E-selectin gene and acute myocardial infarction (AMI) in local Han peoples. Method The genotype of E-selectin were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 168 patients with acute myocardial infarction and 200 healthy controls,and the serum level of E-selectin was measured by enzyme-linked immunosorbent assay (ELISA).Results There was significant difference in frequencies of allele and genotype in S128R polymorphism between acute myocardial infarction and control groups respectively,The relative risk suffered from acute myocardial infarction of SS genotype was 2.234 times of the SR genotype (OR=2.234,95% CI:1.112~4.437),The serum E-selectin level was significantly higher among carriers of SR genotype as compared with SS genotype (41.65?8.87)?g/L vs (34.23?6.72)?g/L,P
3.Cytomegalovirus infection and disease in allogeneic hematopoietic stem cells transplantation
Lu-Jia, DONG ; Mao-Quan, QIN ; Zhi-yong, YU ; Liang-Ping, HU ; Liang-ding, HU ; Shu-juan, LU ; Wei, FAN
Bulletin of The Academy of Military Medical Sciences 2001;25(1):50-53
Objective: To investigate the incidence of CMV infection(CMV-I) and CMV related diseases (CMV-D) after allogeneic hematopoietic stem cells transplantation in 70 consecutive allogeneic hematopoietic stem cells transplantation(allo-HSCT) patients and to search for the optimal prophylactic strategy.Methods: Blood samples were monitored using the CMV pp65 antigenemia assay.Of the 70 patients observed,30 patients with chronic myeloid leukemia[CML:CP(27),AP(2),BC(1)],12 with acute myeloblastic leukemia(AML),10 with acute lymphoblastic leukemia(ALL)and other cases were NHL(3), AA(5), MDS(7), SCLC with pancytopenia (1),CLL(1), and MF (1). Sixty six patients received HLA - identical siblings transplantation and four received tranplants from their HLA- haploidentical donors. Seventy cases included allo-PBPCT (64 cases) , allo-BMT (4 cases) and allo-PB+BMT (2). Before transplantation, all patients and donors received CMV serological examination except 4 pairs of donors/recepients. All 66 patients (3 cases were CMV IgM positive) and 64/66 donors were CMV IgG positive. Results:After transplantation, 64/70 patients developed CMV viremia during monitoring period. Forty three of 70 patients developed CMV-D.Thirty five of them suffered from CMV-associated interstitial pneumonia(CMV-IP). The high peak levels of CMV antigenemia were associated with development of CMV disease . Close correlation was found between acute graft vs host disease(GVHD) and CMV disease. The patients were followed up for 2 to 24 months. The patients who received preemptive therapy(group A)had significantly better outcome than CMV disease group(group B, P=0.0001). Conclusions: The results suggest that CMV antigenemia has high predictive value for subsequent CMV disease and CMV pp65 antigenemia -guided early therapy has particular advantage for avoiding morbidity and mortality caused by CMV disease.
4.The effect of calorie restriction on the expression of liver's gluconeogenesis genes of rats fed a high fat diet.
Ming-Juan LUO ; Lu-Lu CHEN ; Juan ZHENG ; Tian-Shu ZENG ; Xiu-Ling DENG
Chinese Journal of Hepatology 2008;16(2):125-128
OBJECTIVETo observe the effect of calorie restriction on the high fat diet rats mRNA expressions of liver forkhead box O1(FoxO1), phosphoenolpyruvate carboxykinase (PEPCK), glucose-6-phosphatase (G-6-P) and to explore the possible mechanisms.
METHODS24 normal 6-week-old male Wistar rats were randomly divided into three groups: normal chow group (NC, n = 7), high fat diet group (HF, n = 9) and calorie restriction group (CR, n = 8). They were fed for 12 weeks. At the end of the experiment, the rats were sacrificed and their fasting blood glucose (FBG), insulin (INS), triglycerides (TG), total cholesterol (TC) were measured. Their visceral fat (VF) and body weight (BW) were also measured and VF/BW was calculated. Gene expression was investigated by using semi-quantitative RT-PCR methods. Liver histology was studied with HE stained slides.
RESULTSCompared with the NC group, HF group rats developed visceral obesity which was accompanied by higher FBG, plasma INS, TG, and TC. The levels of FoxO1, PEPCK, and G-6-P increased by 18.9%, 33.8%, and 24.6%, respectively (P less than 0.01). Liver steatosis was observed with microscopy. The BW, VF FBG, INS, TG and TC of the CR group rats were lower in comparison to those of the HF group. The levels of FoxO1, PEPCK and G-6-P were lower by 26.6%, 35.0%, 34.3% (P less than 0.01). Meanwhile, liver steatosis was also milder.
CONCLUSIONCalorie restriction can inhibit the expressions of FoxO1, PEPCK and G-6-P, strengthen insulin signal conduction, suppress gluconeogenesis and thus regulate glycometabolism.
Animals ; Caloric Restriction ; Dietary Fats ; Forkhead Transcription Factors ; genetics ; Gene Expression Regulation ; Gluconeogenesis ; genetics ; Glucose-6-Phosphatase ; genetics ; Liver ; metabolism ; Male ; Nerve Tissue Proteins ; genetics ; Phosphoenolpyruvate Carboxykinase (ATP) ; genetics ; Rats ; Rats, Wistar
5.RAPD analysis on different varieties of Rehmannia glutinosa.
Jing-li CHENG ; Lu-qi HUANG ; Ai-juan SHAO ; Shu-fang LIN
China Journal of Chinese Materia Medica 2002;27(7):505-508
OBJECTIVETo provide molecular evidences for its breeding by studying the genetic relationship among varieties of Rehmannia glutinosa.
METHODNineteen varieties were detected by Randomly Amplified Polymorphic DNA(RAPD) markers.
RESULTThe 20 selected primers produced 163 bands, among which 114(69.9%) were polymorphic. A DNA molecular dendrogram was established based on Hierarchical cluster analysis of 163 DNA bands amplified by 20 primers, which divided the 19 varieties into four groups: Group Beijing, Group 85-5, Group Guolimao and the other Group.
CONCLUSION8 varieties of Group Beijing have a close genetic relationship, and so have varieties of Group 85-5, which provides information for Rehmannia glutinosa's breeding.
DNA, Plant ; genetics ; Plant Leaves ; genetics ; Plants, Medicinal ; genetics ; Polymorphism, Genetic ; Random Amplified Polymorphic DNA Technique ; Rehmannia ; genetics
6.Studies on callus culture of Akebia trifoliata.
Guo-Lin SHEN ; Ai-Juan SHAO ; Lu-Qi HUANG ; Shu-Fang LIN
China Journal of Chinese Materia Medica 2007;32(10):899-901
OBJECTIVETo investigate the inductive factors of effect on Akebia trifoliata and establish culture method for A. trifoliata callus.
METHODTo study the possible effective factors of culture condition by comparing with different explantation, nutrient medium, pH, temperature, illumination, growth substance of plant and its ratio.
RESULTThe inductivity of leaves was the highest about 87.5%, followed with the stem section and leafstalk; The inductivity of nutrient medium such as MS, B5 callus was higher than the ones like H, SH and the White callus amended one; It was found that low-grade Phvalue benefits the growth of callus. The experiment result showed that different pH showed little difference in quality. The best condition of culture was 25 degrees C in temperature.
CONCLUSIONThe best culture condition for callus was the leaves as explantation. The A. trifoliata callus culture's best inductive condition was MS +2, 4-D 4.0 mg x L(-1) + NAA 1.0 mg x L(-1) + KT 1.0 mg x L(-1) (pH 5.8), cultural temperature was 25 degrees C, cultivation was dark.
Culture Media ; chemistry ; pharmacology ; Hydrogen-Ion Concentration ; Magnoliopsida ; drug effects ; growth & development ; Plant Leaves ; drug effects ; growth & development ; Plant Stems ; drug effects ; growth & development ; Plants, Medicinal ; drug effects ; growth & development ; Temperature ; Tissue Culture Techniques ; methods
7.Esthetics restoration after rapid orthodontic extrusion of subgingivally fractured incisor: a case report.
Jun JI ; Xiao-ping LUO ; Wei LU ; Yu-juan SHI ; Lie WU ; Cheng-jun SHU
West China Journal of Stomatology 2007;25(2):206-207
A case of a patient with subgingivally fractured incisor was presented. Two weeks after root canal therapy, the subgingival fragment was restored with fiber post, resin core and temporary crown. Gingivoplasty was performed around. after the subgingival fragment had been elevated in the axial direction by means of edgewise fixed appliance. Stabilized and held for 6 months, the incisor was restored with all ceramic crown. Optimal esthetic was achieved when restoration was performed after rapid orthodontic extrusion which had lifted up the fracture line above the level of the gingival line within 14 days. At 6-month follow-up, the periodontal tissues were normal and neither luxation nor relapse was noted.
Crowns
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Dental Porcelain
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Esthetics
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Gingiva
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Humans
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Incisor
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Orthodontic Extrusion
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Post and Core Technique
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Root Canal Therapy
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Tooth Fractures
8.A clinico-pathological analysis of HCV infection in post-liver transplantation patients.
Hui LI ; Zheng-lu WANG ; Shu-ying ZHANG ; Wen-juan CAI ; Zhong-yang SHEN
Chinese Journal of Hepatology 2007;15(8):593-596
OBJECTIVETo investigate the pathological characteristics of HCV infection after liver transplantation.
METHODSThis is a retrospective analysis of the clinico-pathological changes of 73 liver biopsies obtained from 61 patients who had HCV infection (including HCV recurrence and reinfection) after liver transplantation in our center from September 2000 to September 2006.
RESULTSAbnormal enzyme test results due to HCV infection happened on the 9th to the 1553rd post-transplantation surgery day. The serum HCV RNA level was higher than 10(5) copies/ml in 19 cases and between 10(2)-10(5) copies/ml in the other 42 cases. The histological changes in the transplanted livers were hepatocellular degeneration, necrosis and apoptosis, portal infiltrations and fibrosis. They were classified into two stages (early stage and late stage) according to the onset of fibrosis which appeared within 90 days or later after their transplantation in our study. The incidence of predominant portal infiltrates and liver fibrosis in early stage and late stage was 5.7% (2/35) and 94.7% (36/38) (chi2=54.34, P<0.01) and 2.9% (1/35) and 97.4% (37/38) (chi2=61.47, P<0.01) respectively.
CONCLUSIONSPathological features of early stage and late stage hepatitis C infection in transplanted livers are different and they are also different from that in native livers. Liver biopsies are important in clinical staging, evaluation of the severity, and differential diagnosis of post-transplantation HCV infection.
Adult ; Aged ; Female ; Hepatitis C ; etiology ; pathology ; Humans ; Liver Transplantation ; adverse effects ; Male ; Middle Aged ; RNA, Viral ; Retrospective Studies
9.Differential expression of the anti-oncogene ARHI between patients with and without endometriosis.
Jie LI ; Shu-juan WANG ; Lu SUN ; Ya-li LI
Journal of Southern Medical University 2011;31(5):796-800
OBJECTIVETo investigate the expression of the anti-oncogene ARHI in the endometriotic tissue and explore its clinical significance.
METHODSA semiquantitative analysis of the expression of ARHI mRNA and protein in the ectopic and eutopic endometrium of women with endometriosis was conducted using RT-PCR and Western blotting in comparison with that in the endometrium of women without endometriosis. Immunohistochemistry and in situ hybridization were performed for semi qualitative analysis and localization of ARHI expression.
RESULTSThe expression levels of ARHI mRNA and protein differed significantly between the groups. ARHI was expressed at significantly higher levels in ectopic endometrium than in eutopic and normal endometrium (P<0.005), but showed no significant difference between the latter two tissues (Pgt;0.05). The positivity rate ARHI DNA was 97.3% in the endometrium of women without endometriosis, 100% in the ectopic endometrium and 93.8% in the eutopic endometrium of women with endometriosis. immunohistochemistry showed an ARHI positivity of 93.2% in the endometrium of women without endometriosis, 100% in the ectopic endometrium and 92.3% in the eutopic endometrium of women with endometriosis. The expression patterns of ARHI DNA and protein were consistent. Immunohistochemistry in 5 cases of malignant endometriosis showed negative ARHI expression in 4 cases and weak positivity in 1 case.
CONCLUSIONARHI expressions are present in the endometrium and up-regulated in ectopic endometrium, whereas in the ectopic endometrium of patients with malignant endometriosis its expression is often negative, suggesting a role of ARHI in infertility and tumorigenesis of endometriosis.
Adult ; Endometriosis ; metabolism ; pathology ; Endometrium ; metabolism ; pathology ; Female ; Genes, Tumor Suppressor ; Humans ; RNA, Messenger ; genetics ; rho GTP-Binding Proteins ; metabolism
10.Mitochondrial mechanisms of apoptosis of human leukemia K562 cells induced by AVVC-1.
Ru-Qi ZHENG ; Gen-Bao ZHANG ; Lu HUANG ; Kai-Ran MA ; Juan WU ; Shu LI
Journal of Experimental Hematology 2013;21(3):591-595
This study was purpose to investigate apoptosis pathway of leukemia K562 cells induced by anticoagulant fraction from Agkistrodon acutus venom (AVVC-1). The mitochondrial transmembrane potential (ΔΨm) of leukemia K562 cells was detected by flow cytometry with JC-1 single staining. The expression of cytochrome C in the mitochondrial of leukemia K562 cells was analyzed by Western blot after AVVC-1 treatment. The distribution of cytochrome C in leukemia K562 cells was measured by immuno-fluorescence test. The results showed that the potential of mitochondrial membrane decreased after treatment with different concentrations of AVVC-1 (12.5, 25, 50, 100 µg/ml) for 6 h (P < 0.01). The expression level of cytochrome C protein in mitochondria obviously declined after treatment with 30 µg/ml AVVC-1 for 48 h, and the fluorescent intensity of cytochrome C in cytosol was enhanced at the same time. It is concluded that AVVC-1-induced K562 cell apoptosis is related with mitochondrial damage, and cytochrome C may be a useful agent for investigating human leukemia therapy by using AVVC-1.
Agkistrodon
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Animals
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Apoptosis
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drug effects
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Cytochromes c
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metabolism
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Humans
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K562 Cells
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Membrane Potential, Mitochondrial
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drug effects
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Mitochondria
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metabolism
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Snake Venoms
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pharmacology