Cervical Intraepithelial Neoplasia ; diagnosis ; pathology ; surgery ; Colposcopy ; Conization ; Electrosurgery ; Female ; Humans ; Hysterectomy ; methods ; Neoplasm Regression, Spontaneous ; Uterine Cervical Neoplasms ; diagnosis ; pathology ; surgery ; Vaginal Smears

?AlM:To investigate the sensitive parameters of the anterior chamber changes with Pentacam anterior segment analysis system before and after laser peripheral iridectomy (LPl) in primary angle-closure suspetive (PACS).
? METHODS: Sixty eyes of 33 PACS patients were enrolled in this study. Pentacam examination was performed before and 1d after LPl to measure the central anterior chamber depth ( CACD ) , the peripheral anterior chamber depth ( PACD ) , the anterior chamber volume ( ACV) and the peripheral anterior chamber angle ( ACA) . Statistical analysis used paired t test.
?RESULTS: There was no statistical significance on the changes of ACD. PACD and ACV increased significantly between before and 1d after LPl. ACA was widened from (22. 26o±5. 18o) to (26. 42o±5. 20o), which were increased significantly between before and 1d after LPl.
?CONCLUSlON: LPl can deepen the PACD and increase the ACV in PACS. PACD and ACV are the sensitive parameters of the anterior chamber changes with Pentacam anterior segment analysis system.

Humans ; Nasopharyngeal Neoplasms ; classification ; pathology ; Neoplasm Staging ; World Health Organization

Adult ; Humans ; Male ; Occupational Diseases ; Pulmonary Aspergillosis

Background Establising the culture model of Müller cells for obtaining the highly putified target cells is essential for the study about the physiology and pathology of retinal Müller cells. The exsiting purifing method for culturing Müller cells is dissatisfactory. Objective This study was to establish a method to obtain high purifing Müller cells. Methods The retina from 5 clean newborn SD rats were isolated and digested by 0. 01% trypsin and cultured in DMEM containing 10% fetal bovine serum. The cellular suspension was then prepared,and the target cells were screened using flow cytometry based on the size and the quantity of cells. Cultured and passaged cells were identified by transmission electron microscope and light microscope. Immunocytochemistry was used to detecte the expression of GFAP in cultured cells for the determination of type and purity of the cells. Results The cells showed the similar shape to retinal Müller cells after primarily culture with the large volume, and some small other types of cells could been seen. The growth of cells was quickly 3 weeks later. The fibroblasts were removed using sticking-wall by steps,and neurons were eliminated following passage. Aboundent of cellular organs were seen under the transmission electron microscope. The positive response rate of the cells for CFAP was 100%. Conclution Flow cytometry offer a rapid and feasible approach for purifying Muller cell and it builds the foundation for further study about Müller cells.

Objective To detect the effect of leptin on proliferation and c.Myc mRNA expression of human colon carcinoma HT-29 cell line and investigate the role of Leptin in the development of the HT-29 cell line.Methods Human colon carcinoma HT-29 cell line was cultured in vitro.After treatment with various concentration of Leptin for 72h.MTr was used to detect the proliferative and inhibitive status.And c-myc mRNA-expression Was detected by RT-PCR.Results After treatment with various concentration of Leptin.The cell pmlifemtion and c-myc mRNA expression Wag obviously promoted,compared with the control group.The effect wag in a time-dose-dependent manner within a certain range.Conclusion Leptin can improve cell proliferation and c-myc gene expression level in HT-29 cell line.Promoting the c-myc gene expression level may be one of the reasons that Leptin improves the proliferation of the human colon carcinoma HT-29 cell line.

Child ; Electroacupuncture ; adverse effects ; instrumentation ; Electrolysis ; Equipment Failure ; Equipment and Supplies ; adverse effects ; Humans ; Male

Antigens, CD34 ; metabolism ; Craniotomy ; Diagnosis, Differential ; Follow-Up Studies ; Hemangiosarcoma ; metabolism ; pathology ; surgery ; Humans ; Male ; Middle Aged ; Skull ; pathology ; surgery ; Skull Neoplasms ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Animals ; Autophagy ; Humans ; Myocardial Ischemia ; physiopathology ; Myocardial Reperfusion Injury ; physiopathology

Objective: To study the non-alkaloid constituents from the stems and leaves of Alstonia mairei. Methods: The chemical constituents were separated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies, and preparative HPLC. Their structures were determined by physicochemical properties, spectral data, as well as comparisons with the data in literature. Results: Eighteen compounds were isolated from the petroleum ether fraction of 90% ethanol extract from the stems and leaves of A. mairei, and identified as lupeol (1), 30-oxo-lupeol (2), lupenyl acetate (3), α-amyrin (4), α-amyrenone (5), 23-hydroxyursolic acid (6), β-amyrin (7), β-amyrenone (8), maslinic acid (9), friedelinol (10), friedelin (11), tectochrysin (12), 5,6-dihydroxy-7,4'- dimethoxyflavone (13), 5,3'-dihydroxy-7,4'-dimethoxyflavone (14), 7-hydroxy-7,5,3',4'-trimethoxyflavone (15), 5,7,3',4'- tetramethoxyflavone (16), 5-hydroxy-6,7,8,4-tetramethoxyflavone (17), and 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone (18). Conclusion: All compounds are isolated from A. mairei for the first time. In addition to compound 1, the other compounds are isolated from the plants of Alstonia R. Br. for the first time.