Aged ; Animals ; Climacteric ; drug effects ; Disease Models, Animal ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Integrative Medicine ; methods ; Middle Aged ; Perimenopause ; Periodontitis ; drug therapy ; Phytotherapy ; Rats ; Rats, Sprague-Dawley

Objective To investigate neural correlates of directed forgetting. Methods ERP was recorded from 12 healthy postgraduates while they performed a directed forgetting task based on the item-cueing method. During the study phase, each word was followed by a cue indicated that it need to be remembered, to be forgotten or just to be viewed. During the test phase, subjects were asked to make an old/new recognition response. One-way ANOVA of repeated-measurement and paired comparisons were carried out for the cue factor. Results Both of the accuracy and reaction time of the TBR group were significantly superior to those of the other two groups. No significant behavioral effect was found between the TBF group and the TBV group. In the study phase, statistical parametric mapping of ERP suggested the cue effects were presented in the frontal poles ( 200～300 ms ) and the parietal regions (300～400 ms) ,the left fronto-parietal and the right occipital region (450～700 ms). The difference between the TBF and the TBV was significant in the left parietal area (500～525 ms) and has the significant trend in the right frontal area ( 350～600 ms). In the retrieval phase, the old/new effects of ERP occurred in the left fro-nto-temporal (300～450 ms) for the TBF cue. Conclusions The TBF cue and the TBV cue have the equivalent behavioral performance. During the study period, the activation of the right frontal lobe and the left parietal suggests the active inhibition evoked by the TBF cue. And the TBR cue can enhance the rehearsal function of left hemisphere and improve the memory performance of words. The retrieval of the items of TBF is based on more familiarity and less recollection.

Alkylphenols(APs) , the second most widely used commercial non-ionic surfactants in the world, are the main degradated productions of alkylphenol ethoxylates (APEs). The structure, biodegradation, transportation and distribution in environment, environmental monitoring and management are reviewed in this paper. Many meaurement methods of APs show that APs possess estrogenoid effects, that mainly disturb endocrinal and reproductive system, especially, estrogen-dependent organs. APs are associated with decreased fertility, reproductive disfunction, inferbility and cancer formation. APs are able to bind estrogen receptors, to imitate or antagonize estrogen effects, to disturb formation and metabolism of endogenous hormones and receptors. APs are also able to change the structure, quantity and function of cells and to disturb the kinetics of cellcycle at cytological and molecular levels. The more work on epidemiological study and mechanism of APs are needed. Management program for controlling environmental pollution caused by APs is being speedily investigated and put into practice progressively. It is necessary to restrict the use of APEs.

Background and purpose:Hypoxic cells in tumors are of chemoresistant and anti-apoptoic.Our study was focused on the effects of hypoxia on expression of P-glycoprotein(P-gp) and cell apoptosis in human lung adenocarcinoma A549 cell line(wtP53),in order to explore the probable mechanism of hypoxia in apoptic tumor cells.Methods:A549 cell line(human lung adenocarcinoma) was cultured under hypoxic atmosphere(2% O_2) for 24 h,the expression of hypoxia inducible factor-1?、P-gp and P53 protein was detected by immunohistochemistry,after cells under hypoxic atmosphere were exposed to Adriamycin for 24 h,the cell survival rate was detected by MTT,and apoptosis of by flow cytometer.Results:① The expression of hypoxia inducible factor-1?、P-gp and P53 protein in hypoxic cells were higher than the expression in anoxia ,and correlation between the expression of HIF-1? and P-gp or P53 protein was observed(P

Objective To evaluate the risk factors of myasthenic crisis after thymectomy for myasthenia gravis.Methods Meta-analysis was performed to screen risk factors of myasthenic crisis after thymectomy for myasthenia gravis based on 21 associated articles published and carried out using Stata12.0.Results Twenty one studies were identified, including 3 092 patients and 698 patients developed myasthenic crisis after thymectomy.Complicated with thymoma(OR =3.93, 95％ CI 2.37-6.50, P =0.000), Ossermen classification (OR =0.12, 95 ％ CI 0.09-0.17 , P =0.000) , history of preoperative myasthenic crisis (OR =5.36, 95 ％ CI 3.82-7.51, P =0.000) , use steroid hormonea (OR =1.75,95 ％ CI 1.26-2.43, P =0.001) , postopertive lung infection(OR =4.27,95％ CI 1.63-11.18, P =0.003) , operation methods(OR =0.34,95％ CI 0.21-0.56, P =0.000) , the dose of preoperative anticholinesterase drags (OR =0.35, 95 ％ CI 0.18-0.66, P =0.000) ,pulmonary function(OR =0.18, 95％ CI 0.06-0.57, P =0.004), preoperative serum level of anti-acetylcholine receptor antibody(OR =0.06, 95％ CI0.03-0.13, P =0.000)were associated with myasthenic crisis after thymectomy.Gender, course of disease were not associated with myasthenic crisis after thymectomy.Conclusion With thymoma, Ossermen classification, history of preoperative myasthenic crisis, use steroid hormonea, operation methods, the high dose of preoperative anticholinesterase drugs, poor pulmonary function, postopertive lung infection, high preoperative serum level of anti-acetylcholine receptor antibody are risk factors affecting myasthenic crisis after thymectomy.

Objective To investigate the clinical effect of orthokeratology (ortho-k)during night and wearing frameglass during daytime for treating high myopic in teenagers.Methods 53 teenager cases (aged 10 - 14 years old)of high myopia with spherical equivalent degree -6.00--8.00 diopters (D)in our hospital were performed the retrospective analysis,among them,23 cases were the combined wearing of ortho-k and frameglass and 30 cases were simple wearing of frameglass.The changes of eye axi-al length at 6,12,18 24 months before and after wearing frameglass were measured and compared between the two groups.The spherical equivalent degrees were compared between before wearing frameglass and at 2 years after wearing frameglass.Results The spherical equivalent degree after stopping wearing for 1 month after 2-year wearing in the ortho-k and frameglass group was(-7.28±0.57)D,which after 2 years in the frameglass group was (-7.80±0.68)D,the 2-year spherical equivalent degrees in the or-tho-k and frameglass group and the frameglass group were increased by (-0.29 ±0.22)D and(-1.07 ±0.41)D respectively,the difference between the two groups was statistically significant(P <0.01);the eye axial growths were(0.12±0.04)mm and(0.37± 0.13)mms respectively,the difference between the two groups was statistically significant (P <0.01).Conclusion The combina-tion of ortho-k during night and frameglass during daytime can effectively relieve the myopia increase and eye axis elongation in the patients with high myopia and control the myopia development to some extent.

Humans ; Tinnitus ; diagnosis ; therapy

As an efficient gene transfer vehicle lentiviral vector has been widely used in therapy research. Comparing with other retrovirus vectors, lentiviral vectors have the unique ablility of transfecting nondividing cells and terminal differentiated cells. In addition, lentiviral vectors can accommodate two or more promoters and can carry larger foreign gene insertions. Now the new generation of lentiviral vectors encoding transcriptional control sequence provides effective means for the regulation of foreign gene expression. The development of lentiviral vectors and its application in the gene therapy field were summarized.

Abstract: Objective　 This article aims to present a rare case of severe fever with thrombocytopenia syndrome (SFTS) complicated by with bacteraemia caused by Campylobacter jejuni, and to discuss the pathogenic characteristics, culture methods, clinical features and treatment points of Campylobacter jejuni and the patient's outcome, with a view to raising clinical awareness of blood culture and providing experience for the treatment of this disease. Methods　The clinical data of a case with SFTS complicated by bacteremia caused by Campylobacter jejuni admitted to Weihai Municipal Hospital were collected and the diagnostic process of the pathogenic bacteria as well as the treatment plan were retrospectively analysed. Results　The patient was a female who had been bitten by a tick bite half a month ago and presented to the hospital on 30th August with a fever, vague pain in the peribulbar abdomen and diarrhea for 5 days. Laboratory tests showed leukopenia and thrombocytopenia, and nucleic acid detection for SFTS was positive, resulting in a diagnosis of SFTS. After a week of antiviral treatment with ribavirin and symptomatic treatment, the patient suddenly experienced high fever at night, with a temperature reaching 39.5 °C. Blood cultures were immediately taken from both sides of the double bottle. Bilateral anaerobic bottles were tested for positive after 53.06 hours, and Gram-negative Campylobacter was cultured anaerobically in a transfer blood plate and further identified as Campylobacter jejuni using mass spectrometry MALDI-TOF MS. Vancomycin was stopped clinically on the basis of bacterial pathogenesis and meropenem was used for anti-infection and symptomatic treatment. During the treatment, blood culture and nucleic acid detection for SFTS turned negative, and the patient's symptoms improved. After normal results were achieved in the follow-up testing, the patient was discharged. Conclusions　This case serves as a reminder that Campylobacter jejuni not only causes intestinal infections, but can also lead to extra-intestinal infections in immunocompromised individuals. Clinical and laboratory personnel should increase their recognition of Campylobacter jejuni, prioritize blood culture methods, and utilize a multidisciplinary approach in diagnosis and treatment.

Humans ; Leukemia, Large Granular Lymphocytic