Bone marrow mesenchymal stem cells (BMSCs) are a group of pluripotential non-hematopoietic somatic stem cells niched in bone marrow.With the characteristics of stable genetic traits,pluripotential in differentiation,easy to isolate from source tissue,and fast to proliferate when cultured in vitro,BMSCs are currently attracting extensive research interests,and considered to be one of the most promising candidates in corneal tissue engineering.At present,many research groups,domestic and abroad,have reported that BMSCs can not only differentiate into corneal limbal stem cells,corneal epithelial cells,and corneal endothelial cells,but also play an important role in ocular surface repair.However,the successful application of BMSCs in cornea usually depends on the correct selection of supporting materials or scaffold,such as xenogeneic corneal stroma and amniotic membrane.Other unsolved problems in BMSCs-related corneal tissue engineering include the molecular biologic mechanism underlying the directional differentiation from BMSCs to corneal cells,the standards to identify BMSCs from differentiated corneal cells,the optimal scaffold materials and the potential tumorigenicity with grafting of transformed or undifferentiated BMSCs.This paper reviewed the progresses and issues of corneal tissue engineering with BMSCs.

Antineoplastic Agents ; therapeutic use ; Arsenicals ; therapeutic use ; Humans ; Myelodysplastic Syndromes ; drug therapy ; Oxides ; therapeutic use

Cardiovascular Diseases ; Follistatin-Related Proteins ; physiology ; Humans

Bone Demineralization, Pathologic ; prevention & control ; Dental Caries ; prevention & control ; therapy ; Dental Restoration, Permanent ; Education, Dental ; Gingivitis ; prevention & control ; Humans ; Orthodontics, Corrective ; Preventive Dentistry ; education

Objective To assess the possibility of using arterial pressure waveform or pulse oximetry plethysmographic waveform variation to estimate the pulmonary arterial wedge pressure (PAWP) Methods Fourteen ASA Ⅰ Ⅱ patients aged 33 69 years and weighing (62 0?9 5)kg scheduled for elective abdominal tumor surgery were studied Their Hb exceeded 120g/L and Hct 35% Anesthesia was induced with midazolam 0 04mg/kg atropine 8?g/kg,fentanyl 2?g/kg,propofol 2mg/kg and vecuronium 0 1mg/kg and maintained with isoflurane The patients were intubated and mechanically ventilated and P ET CO 2 was maintained at 4 67 5 33 kPa Radial artery was connulated for arterial pressure waveform monitoring and Swan Ganz catheter was inserted via right internal jugular vein and connected to continuous cardiac output monitor (VGS2,Baxter,USA) for hemodynamic monitoring Hypervolumic hemodilution was performed after induction of anesthesia and intubation with crystalloid and colloid (1:1) infused at a rate of 0 7ml?kg -1 ?min -1 PAWP, systolic pressure variation (SPV), delta down (dDown), SPV plet and dDown plet and other hemodynamic parameters were measured and recorded when total fluid volume (crystalloid and colloid) infused reached 10ml/kg and 20 ml/kg and at the end of operation, CVP was maintained at 10 12mm/kg during operation Systolic blood pressure at the end of Valsalva maneuver (airway pressure was kept at 30 cmH 2O) and the systolic pressure before the Valsalva maneuver during apnea were used to calculate arterial pressure ratio (APR) Results APR,SPV,dDown,SPV plet and dDown plet all correlated well with PAWP (r=o 7174,-0 6951,-0 680-0 5216 and 0 6237 respectively P

Microcystins (MCs) is a potent cyclic heptapeptide hepatotoxin produced by the blue-green algae,Microcystis aeruginosa. The pollution caused by microcystins has become a global concern. Facing to MCs-induced health hazards and potential economic losses,it has an important practical significance to study the effective chemoprotectants against MCs. In this paper,both domestic and abroad researches on the chemoprotectants (silymarin,curcumin,green tea polyphenols,cyclosporin-A,rifampin,trace element supplementation) against MCs were reviewed.

Aim To provide experimental basis to study Human Cytomegalovirus(HCMV)infected disease and filtrate anti-virus drugs, HCMV acute interstitial pneumonia murine model was established. Methods Balb/c mice were infected with HCMV AD_ 169 strain. The rate of breath and weight of mice were observed, specific anti-HCMV antibody in serum was detected by ELISA, and viral isolation, Polymerase chain reaction(PCR) and pathological examination of lung tissue were performed aswell. Results Compared with the control group, the rate of breath of mice in the group infected with HCMV increased, while their weight decreased (P

Objective To evaluate the efficacy of 2-year treatment of interferon alpha combined with lamivudine therapy in chronic hepatitis B patients. Methods From 1999 to 2001 87 patients were enrolled and randomized into two groups.36 patients received interferon alpha and lamivudine simultaneously for 26 weeks,then lamivudine alone for 76 weeks.51 patients received lamivudine alone for 104 weeks.The efficacy was evaluated by biochemical and virological parameters.The YMDD motif was detected in both groups. Results There was no difference between two groups in HBV DNA response ( P =0.24).Proportion of HBeAg/Anti-HBe seroconversion in combination group was significantly higher than lamivudine group (38.9% vs 17.6%)( P =0.03).HBV YMDD mutation rate in combination group was lower than in lamivudine group (22.2% vs 43.1%, P =0.04). Conclusion The efficacy of combination therapy appeares to be better than lamivudine monotherapy.Combination treatment may delay or diminish the development of HBV YMDD mutation.

Objective To investigate the expression of the vascular endothelial growth factor (VEGF) and mucin MUC5AC in nasal mucosa before and after chronic rhinitis - sinusitis and nasal polyps - endoscopic sinus surgery.Methods 75 cases chronic rhinitis - sinusitis and nasal polyps - endoscopic sinus surgery patients were selected as nasal polyps group and 75 cases of nasal bone fracture or epistaxis patients as the control group from January 2012 to January 2015. Took the samples of nasal polyps before surgery and the maxillary sinus mucosa specimens after surgery six weeks of nasal polyps’ patients and on the edge of the inferior turbinate mucosa specimens of the control group to detect eosinophil count by HE staining, and detect the expression of VEGF and mucin MUC5AC by immunohistochemical staining.Results The specimens eosinophils of preoperative nasal polyp group and postoperative nasal polyp group were higher than that of control group (P < 0.05), the nasal eosinophils of postoperative nasal polyp group was lower than that of preoperative nasal polyp group (P < 0.05). The expression of specimens VEGF and mucin MUC5AC area percentages in preoperative nasal polyp group and postoperative nasal polyp group were higher than that in control group (P < 0.05), the expression of nasal VEGF and mucin MUC5AC area percentages in the postoperative nasal polyp group was lower than that of preoperative nasal polyp group (P< 0.05).Conclusion Eosinophil count and the expression levels of VEGF and mucin MUC5AC of nasal mucosa in chronic rhinitis - sinusitis and nasal polyps - endoscopic sinus surgery preoperative are higher, and reduce at postoperative six weeks, VEGF and mucin MUC5AC may be involved nasal repair.

Background Inflammation and adipogenesis are two parallel processes with increasing activity in severe thyroid-associated ophthalmopathy(TAO),and mevastatin was proved to have the inhibiting effect on the differentiation of adipose.Objective The aim of this work was to investigate the effects of mevastatin on the expression of cyclooxygenase-2(COX-2)and peroxisome proliferator activated receptor-γ(PPAR-γ)and differentiation of TAO-derived orbital preadipocytes,and explore its modulation effects on lipopolysaccharide(LPS)induced inflammation and the differentiation of TAO-derived orbital preadipocytes in vitro.Methods The retroorbital adipose tissue was obtained from 4 TAO patients during the surgery.The orbital fibroblasts were cultured from orbital adipose tissues using explant culture method.To study the suppressing effect of mevastatin on inflammatory response,cultured cells were divided into 5 groups.The 1000 μg/L LPS orbital fibroblasts were stimulated for 8 hours in group A,and 1000 μg/L LPS combined with 5 μmol/L,10 μmoL/L or 20 μmoL/L mevastatin were used respectively for the substitute in the group B,group C and group D.The orbital fibroblasts in group E were cultured routinely without any intervention as control.To observe the inhibiting effect of mevastatin on the differentiation of adipose,the group A were then subdivided into group A1-A6.After 1000 μg/L LPS was used to treat the cells for 8 hours,the ceils were induced to differentiate into adipocytes.All orbital preadipocytes from A1 to A6 were stimulated to differentiate into mature adipocytes with cocktail differentiation medium for a 10-day duration.During the procedure,group A2,A3 and A4 were interfered with 5,10 or 20 μmol/L mevastatin,and in the group A5 and A6,10 μmol/L mevastatin were added at the fourth day or eighth day.Intracellular fat accumulation in differentiated adipocytes was determined by oil red O staining.The absorption(A492 nm)was measured in the ceils by enzyme-linked immunosorbent assay(ELISA).Expression of COX-2 and PPAR-γ mRNA was detected by reverse transcription polymerase chain reaction(RT-PCR),and the expression of COX-2 and PPAR-γ protein was detected by Westernblot.The level of PGE2 in the supernatant was detected by ELISA.Results The expression of COX-2 protein and mRNA as well as the PGE2 levels in B,C,D group decreased markedly in comparison with those in A group(P＜0.05).With the increase of mevastatin concentration,the expression of COX-2 protein and mRNA as well as the PGE2 levels in B,C,D groups decreased successively(F =228.380,101.745,1586.881,P＜0.05).The expression of COX-2 protein and mRNA and PGE2 levels in E group were lower significantly than those in A,B and C groups(P＜0.05),but no significant differences were found between E group and D group(P＞0.05).The A492 value and the expressions of PPAR-γ protein and mRNA in differentiated cells showed the successively decrease in A1-A4 group with the elevation of mevastatin concentration(P＜0.05),and the evidently decreased A492 value and the expressions of PPAR-γ protein and mRNA also were seen in A1 and A5 groups compared with A3 group(P ＜ 0.05).Conclusions Mevastatin inhibits LPS-induced COX-2 expression,PPAR-γ expression,PGE2 secretion and differentiation of TAO-derived orbital fibroblasts in vitro in dose-dependent manner.Mevastatin plays these effect more prominently in early stage of adipocytes differentiation.