Objective: To investigate clinical significance of survivin expression,cellular apoptosis in ovarian cancer.Method: The apoptosis in sit ovarian cancer were identified by the terminal deoxynucleotidy transfer mediated dUTP-biotin nick end labeling(TUNEL) and survivin expression was detected by immunohistologic staining in 50 cases of ovarian cancer.Result: Survivin was expressed in 36 of 50 ovarian cancer(72%).The correlation was found between survivin expression,cellular apoptosis.The 5 year survival rate in the group of survivin-negative cases was significant higher than in the group of survivin-positive cases(P

OBJECTIVE To investigate the antimicrobial-resistant profile of Staphylococcus aureus isolated from clinical specimens. METHODS The isolates of S.aureus were obtained from all kinds of clinical specimens by routine methods.The identification and susceptibility test of the isolates were determined by Automated Microbiology Analyzer.Disk-diffusion was used for detecting meticillin-resistant Staphylococcus aureus(MRSA). RESULTS The isolates were mainly isolated from sputa and urines.62.1% of isolates were referred as MRSA.All isolates of MRSA were resistant to ampicillin/sulbactam,penicillin G and cefazolin.No isolates of MSSA were resistant to above drugs.All isolates were susceptible to nitrofurantoin and vancomycin.The resistant rates of the isolates of MRSA to clindamycin,erythromycin,gentamicin,tetracycline,rifampin,sulfamethoxazole and levofloxacin were 70.7%,86.6%,87.8%,82.9%,42.7%,30.5% and 91.5%,respectively,those of MSSA were 31.3%,41.7%,20.8%,20.8%,10.4%,29.2% and 6.3%. CONCLUSIONS The antimicrobial resistance of MRSA is serious,but the antibiotics have high activity to the isolates of MSSA except penicillin G in vitro.Vancomycin has very high activity to the isolates of MRSA in vitro.

OBJECTIVE:To observe therapeutic efficacy and ADR of oral administration and external application of Qili san in the treatment of trauma-induced swelling and pain. METHODS:70 traumatic patients were randomly divided into treatment group and control group with 35 patients in each group. Treatment group was given Qili san(1.5 g/time,2 times/d)orally,and ap-plied to affected area(blended with 75% alcohol to make paste,1 time/d);control group was only given Qili san(1.5 g/time,2 times/d) orally;a treatment course lasted for 7 d. Pain degree score,pain duration,swelling degree score and improvement rate, total effective rate and ADR were compared between 2 groups before and after treatment. RESULTS:Compared with before treat-ment,pain degree score,pain duration and swelling degree score of 2 groups were decreased after treatment;there was statistical significance(P<0.01 or P<0.05);clinical symptom of treatment group was better than that of control group;there was statistical significance(P<0.01 or P<0.05). There was statistically significant difference in the improvement rate of swelling and total effec-tive rate between 2 groups after treatment(P<0.01 or P<0.05). No obvious ADR was found during treatment. CONCLUSIONS:Oral administration and external application of Qili san is safe and effective for trauma-induced swelling and pain,and its therapeu-tic efficacy is better than only oral administration.

Objective: To investigate the clinical effect of ginger-partitioned moxibustion at Zigong (EX-CA 1) for primary dysmenorrhea. Methods: A total of 112 patients with primary dysmenorrhea were randomized into an observation group and a control group according to their visiting sequence, 56 cases in each group. Patients in the observation group received ginger-partitioned moxibustion at Zigong (EX-CA 1), while patients in the control group received oral intake of analgesic. For both groups, treatment started 1 week before menstruation and lasted for 3 menstrual cycles, continued by a 3-month follow-up visit, then the clinical efficacy was evaluated. Results: By the end of treatment, symptom score in the observation group was lower than that in the control group, showing a statistical significance (P<0.05). After 3 months of treatment, the value of prostaglandin F2a (PGF2α), systolic-to-diastolic peak velocity ratio (S/D), resistance index (RI) and pulsatility index (PI) in the observation group were significantly higher than those in the control group, showing statistical significances (all P<0.01).The recovery rate in the observation group was higher than that in the control group, showing a statistical significance (P<0.05). Conclusion: Ginger-partitioned moxibustion at Zigong (EX-CA 1) for primary dysmenorrhea is a combination of the merits of warming function of moxibustion, dissipating function of ginger and stimulation of acupoint, and is better than oral intake of analgesic.

In the past, treatment of acupuncture and moxibustion always were emphasized on improving the strength of muscle. In fact, spasticity is the key point. This paper summarized recent studies on therapeutic effect of acupuncture and moxibustion on spastic paralysis after stroke.

Acupuncture Points ; Administration, Cutaneous ; Adult ; Aged ; Drugs, Chinese Herbal ; administration & dosage ; Facial Paralysis ; drug therapy ; Female ; Humans ; Male ; Middle Aged ; Treatment Outcome ; Young Adult

Phellinus is a kind of rare medicinal fungus that has a variety of physiological activities include anti-cancer, anti-liver fibrosisa, antioxidant and so on. Phellinus contains polysaccharides, steroids, terpenoids, flavonoids, pyrone, furan, alkaloids and other substances. Polysaccharide extracts of phellinus showed obvious antitumor effect and has been a hot research field in recent years. It was also found other extracts of phellinus such as ethyl acetate extract exhibited anticancer activity. Thus, the antitumor effect of different extract, especially the anti-cancer mechanism were discussed in this review.
Antineoplastic Agents ; pharmacology ; Basidiomycota ; Cytokines ; analysis ; Neoplasm Metastasis ; prevention & control

Objective To develop an approach for a rapid extraction of genomic DNA fragments from serum.Methods Serum specimen from human was processed using high temperature denaturation and was centrifuged, then using various primer sets to amplify the DNA fragment extracted, so as to verify the efficiency of this DNA extraction method.Results The templates preparing from this DNA extraction method were able to guarantee the amplification of fragments shorter than 400 base pairs.Conclusion This method is simple, rapid and inexpensive.These genomic DNA fragment extracted can be utilized to amplify definite DNA template for polymerase chain reaction.

Cervical Intraepithelial Neoplasia ; diagnosis ; pathology ; surgery ; Colposcopy ; Conization ; Electrosurgery ; Female ; Humans ; Hysterectomy ; methods ; Neoplasm Regression, Spontaneous ; Uterine Cervical Neoplasms ; diagnosis ; pathology ; surgery ; Vaginal Smears

Anemia, Hemolytic, Autoimmune ; therapy ; Antibodies, Monoclonal, Murine-Derived ; therapeutic use ; Female ; Humans ; Infant ; Rituximab