Objective To investigatethe the clinical efifcacy and immunomodulatory effect of paclitaxel and cisplatin in treatment of advanced ovarian carcinoma by different administration ways. Methods 94 patients with advanced ovarian carcinoma were randomly divided into observation group and control group. Patients in observation group (47 cases) were treats with paclitaxel plus cisplation by intraperitoneal infusion chemotherapy, the other patients (47 cases) who are in control group were treats with paclitaxel plus cisplation by intravenous chemotherapy. Two groups used the same medicinal dose, and 3 weeks as a course. After two courses, therapeutic effect and drug toxicity side effects were evaluated by measuring positive rate changes of peripheral blood immune cells (CD 4+CD 25+cell), the CD 4+/CD 8+ratio changes, progression-free survival and 3-year survival rate. Results The efifcacy of patients in two groups were evaluated objectively. Observation group of RR and DC were respectively 78.7%and 91.5%, signiifcantly higher than that of control group. To the end of follow-up period, two groups of progression-free surial compared with a signiifcant difference. After two courses of chemotherapy, positive rate of immune cells (CD 4+CD 25+cell) declined had signiifcant difference compared with before treatment. After two courses of chemotherapy, two groups of CD 4+/CD 8+ratio were increased, compared with before treatment, which is a signiifcant difference. The main adverse reactions of the two groups were myelosuppression and neurotoxicity, but there were no termination of tolerance for adverse reactions. Conclusion The programs with paclitaxel plus cisplatin peritoneal perfusion chemotherapy for advanced ovarian cancer which is effective, small toxic and safe, is worthy of clinical application.

0 05).Teh average interal from start of labor induction to onset of labor in gruou A was significantly shorter than that in group B(P

OBJECTIVE To investigate the antimicrobial-resistant profile of Staphylococcus aureus isolated from clinical specimens. METHODS The isolates of S.aureus were obtained from all kinds of clinical specimens by routine methods.The identification and susceptibility test of the isolates were determined by Automated Microbiology Analyzer.Disk-diffusion was used for detecting meticillin-resistant Staphylococcus aureus(MRSA). RESULTS The isolates were mainly isolated from sputa and urines.62.1% of isolates were referred as MRSA.All isolates of MRSA were resistant to ampicillin/sulbactam,penicillin G and cefazolin.No isolates of MSSA were resistant to above drugs.All isolates were susceptible to nitrofurantoin and vancomycin.The resistant rates of the isolates of MRSA to clindamycin,erythromycin,gentamicin,tetracycline,rifampin,sulfamethoxazole and levofloxacin were 70.7%,86.6%,87.8%,82.9%,42.7%,30.5% and 91.5%,respectively,those of MSSA were 31.3%,41.7%,20.8%,20.8%,10.4%,29.2% and 6.3%. CONCLUSIONS The antimicrobial resistance of MRSA is serious,but the antibiotics have high activity to the isolates of MSSA except penicillin G in vitro.Vancomycin has very high activity to the isolates of MRSA in vitro.

Pulmonary cavitary lesions in children consist of a group of heterogeneous diseases, mainly caused by infections, and their imaging manifestations can be similar.It is clinically difficult to distinguish them from other lesions such as bullae, cyst, and emphysema.Some scholars have advanced a concept about thin wall(4 mm or less) and thick wall(more than 4mm).People tried to make this distinction by defining cyst as a thin wall and cavity as a thick wall, but there are considerable overlaps between the two categories in etiology and pathophysiology.They are sometimes difficult to distinguish for imageology, and it is still necessary to find the cause of the disease based on the characteristics.This review divides etiology into two categories: infectious and non-infectious etiology.Combined with chest imaging examination, the purpose is to analyze and summarize the features of pulmonary cavitary lesions in children, and provide a diagnostic idea for differentiating various pulmonary cavities to guide clinical treatment.

Objective To determine risk factors and treatments of capillary leak syndrome (CLS) in infants with congenital heart disease(CHD)after extracorporeal bypass (GBP) . Method A retrospective study of 38 infants with CIS and another 150 cases without CLS of a random choice who underwent extracoiporeal bypass from June 2003 to July 2007 was carried out.Several risk factors with statistical significance were screened out with uni-varite logistic regression analysis, and the independent rask factors of CLS were determined with inultivariate step-wise logistic regression analysis. The outcome of CIS infants was compared with infants of control group. Results Logistic analysis showed the risk factors of CLS were the duration of GBP ( OR = 10. 353) , type of CHD ( OR = 6. 912), age ( OR = 6. 254) and temperature of CBP ( OR = 4. 151) . Of the CLS infants cohort, 10 cases underwent peritoneal dialysis and 4 cases died. Conclusions The risk factors of CLS in infants after CBP are the length of time consumed during CBP, type of CHD, age and temperature of CBP.

ObjectiveTO study the mechanism of xingnao kaiqiao acupuncture for brain occlusion. Methodusing one-side middle cerebral artery occlusion(MCAo)rats by thermoregulation as focal cerebral ischemis experimental animal. The dynamic changes of c-fosprotein in sichemic region of brain tissue were observed by imnunohistochemitry method. Meanwhile the effect of acupuncture was stud-ied. ResultThe outcome of xingnao kaiqiao group was better than that of rourine group. Acupuncture could enhance the expression of c-fosprotein in all stages of ischemia, promote the stress ability of neuron produce adaptive changes to brain sichemia and improve recovery of braintissue. ConclusionThe dffect of xingnao kaiqiao acupuncture was better than that of routine acupuncture.

Lactic acid bacteria (LAB) are traditional dairy strains which have a long history of safe use. They are a kind of food-grade microorganisms that can be used in industry to produce lots of food and drink, such as yoghourt. In the last two decades the physiology and genetics of these bacteria have been thoroughly studied. Because of their genetic accessibility and easy to handle, LAB, in addition to their traditional applications, have been extensively developed and used for the expression of heterologous genes. So they have an important foreground in agricultural, medical and many other fields. People have developed a series of food-grade gene expression systems of LAB. LAB, especially their model strain Lactococcus lactis and their most useful food-grade induced expression system--nisin controlled gene expression system NICE and its food-grade inducer nisin, food-grade host, expressing vectors and applications in expressing heterologous proteins of NICE will be introduced.

Objective:To observe the effect of Pidotimod and Thalidomid to enhance the immune response and protective immunity induced by the epitope Vaccines From W2b2a of Toxoplasma gondii in mice.Methods:Mice were immunized intramuscularly with Pidotimod and pcDNA3-W2b2a,thalidomide and pcDNA3-W2b2a,respectively,then the immune response and the survival time of mouse attacked by Toxoplasma gondii were observed.Results:After the immunization,the level of IFN-γin sera of mice inculated with pcDNA3-W2b2a and Pidotimod,pcDNA3-W2b2a and Freund adjuvant,pcDNA3-W2b2a and Thalidomid were significantly higher than pcDNA3-W2b2a (all P<0.05).After the immunization,IgG,CD4+/CD8+T cell ratio ,proliferation of T cell induced by pcDNA3-W2b2a and Pidotimod,pcDNA3-W2b2a and Freund adjuvant were higher than pcDNA3-W2b2a,pcDNA3-W2b2a and thalidomid ( all P<0.05).After challenged with highly virulent tachyzoites,the mean survival time in immunized groups were significantly longer than control group (all P<0.05).Conclusion:Pidotimod ,Thalidomid adjuvant can increase protective immunity of epitope Vaccines From W2b2a of Toxoplasma gondii in mice.

Epidemiological data,clinical observation and animal experiments have shown that cold wave is closely associated with the onset of stroke.When a population with stroke etiology or risk factors is under pre-stroke state,they will have stroke under the influence of various inducing factors.Cold wave is the external factor that causes the body to enter the pre-stroke state and there are many possible mechanisms.The drug intervention of stroke-prone hypertensive rats at 1 week before the cold wave can reduce the occurrence of stroke during cold wave,suggesting that it is of great significance to conduct preventive intervention to the pre-stroke population before the cold wave coming.

The introduction of highly active antiretroviral therapy (HAART) has greatly changed the pattern and natural history of ocular diseases of HIV-infected patients, resulting from the immune recovery and reduction of opportunistic infections. However, ophthalmic complica-tion continues to be concern in AIDS even in the HAART era, especially in developing areas, where absolute majority of HIV-positive patients live. Lack of test facilities and experience, poor conditions of hygiene, different microbiological environment, absence of effective treatment etc., characterize the ophthalmic manifestation of HIV-infected patients in developing countries from that in developed regions and thus pose a great challenge to the ophthalmic treatment in developing area. Not only varied from region to region, ocular complications are distinctive between adults and children. At the same time, the side effects due to the application of HAART pose their own risks of ocular complication and should, therefore, be given more research attention.