Objective To observe the effect of transcutaneous electrical acupoint stimulation on stress reaction in elderly pa-tients with intravenous patient-controlled analgesia after total hip replacement operation.Methods A total of 60 cases of patients with patient-controlled intravenous analgesia spinal anesthesia after total hip replacement surgery were randomly divided into exper-imental group (n=30)and the control group (n=30).The experimental group respectively at the completion of surgery,postopera-tive 4,24,48 h was treated by transcutaneous electrical acupoint stimulation.The serum cortisol and blood glucose was detected in two groups at preoperative 30 min (T1 ),30 min after operation (T2 ),1 d after operation in the morning (T3 ),2 d after operation (T4 ).The pain scores,sedation scores,adverse reactions and analgesic dosage were investigated in two groups after operation.Re-sults In the experimental group,cortisol and blood glucose levels increased compared T2 and T1 (P <0.01 ).Compared with the control group,cortisol and blood glucose levels in the experimental group decreased at T3 and T4 (P <0.05 ).Compared with the control group,the VAS scores was significantly different in the experimental group(P < 0.01 ).The side effects in experimental group were significantly lower than those in the control group(P <0.05).After TEAS treatment,the physic liquor consumption and press the number decreased significantly (P < 0.05 ).Conclusion TEAS technology for elderly total hip replacement operation PCIA could effectively inhibit stress,enhance the analgesic effect,reduce or avoid the occurrence of adverse reactions.

Adolescent ; Child ; Child, Preschool ; DNA Mutational Analysis ; Diagnosis, Differential ; Humans ; Infant ; Infant, Newborn ; Liver Diseases ; diagnosis ; genetics ; pathology ; Lung Diseases ; diagnosis ; genetics ; pathology ; Magnetic Resonance Imaging ; Mutation ; Polycystic Kidney, Autosomal Dominant ; diagnosis ; genetics ; pathology ; Polycystic Kidney, Autosomal Recessive ; diagnosis ; genetics ; pathology ; Prenatal Diagnosis ; Receptors, Cell Surface ; genetics ; Tomography, X-Ray Computed

Objective To explore the adverse effects of formaldehyde(FA)on learning and memory ability of mice and the antagonistic effect of N-acetyl-cysteine(NAC),an antioxidant.Methods Thirty-four ICR mice were randomly divided into four groups,the control(NS,n=8),treated with FA(15 mg/kg,n=9),treated with NAC(100 mg/kg,n=8),treated with FA(15 mg/kg) plus NAC(100 mg/kg,n=9),the treatment was conducted by intraperitoneal injection once a day for seven consecutive days.On the eighth day,the learning and memory ability were tested by using water labyrinth task for seven consecutive days.Results The mice in FA group behaved excited,restless and then turned to repose,moveless and clustering,but this phenomena was not seen in the other groups.There was no significant difference in the body weight of mice among groups.As for learning,latent period in the FA group [(27.15?2.66)s] was significantly longer than that in the control group [(15.83?2.82)s] and the FA+ NAC group[(14.98?2.66)s],and revealed statistical significance(P

Objective:To develop nano-TiO_2 ceramic-coated orthodontic brackets, and to study the surface characters of ceramic film. Methods:TiO_2 nanoparticles were synthesized by the Gel-Sol method. Electron beam physical vapor deposition(e-beam PVD)technology was used to deposit the nano-TiO_2 ceramic film on the surface of the orthodontic brackets, which were manufactured by the Xinya factory, Hangzhou.The surface properties of the nano-TiO_2 ceramic film were observed with scanning electron microscopy(SEM),transmission electron microscopy(TEM) and X ray power diffraction(XRD).Results:The synthesized TiO_2 particles were uniform with the diameter of (6?2) nm. The TiO_2 ceramic film on the orthodontic brackets was 5~8 ?m in thickness, with smooth surface and anatase stratiform structure.Conclusions:The nano- TiO_2 ceramic film has good surface properties and actinochemistry characters without any influence on the slot size.

AIM: To study the effects of cripto antisense oligodeoxynucleotide (ASODN) on telomerase activity in colon cancer LS-174T cells. METHODS: Colon cancer LS-174T cells were treated with cripto ASODN. The expression of cripto mRNA was studied using real-time PCR. The telomerase activity was detected by telomeric repeat amplification protocol enzyme-linked immunoabsordent assay (TRAP-ELISA). The growth of LS-174T cells was observed with soft agar. RESULTS: Cripto ASODN inhibited the growth of LS-174T cells in a dose-dependent manner in soft agar. Cripto ASODN decreased the telomerase activity in colon cancer LS-174T cells, and the effects of repression were time-and dose-dependent. CONCLUSION: Cripto may contribute to the activation of telomerase in colon cancer.

Anodontia ; Humans ; Incontinentia Pigmenti ; therapy

The community - based healthcare service is a main component of healthcare service system in China,and its development is affected by several factors including correlative ethical issues.In order to maintain a long - term and stable development of community - based healthcare service,great importance should be attached to some concerned ethical issues,such as the human - oriented service,intimacy of patients,the interests of disadvantaged group,etc.

Heart ; physiology ; Humans ; Stress, Physiological

Objective To establish the analytical method for fingerprint of Aristolochia manshuriensis by HPLC-DAD-ESI/MS,which can be used as the basis for quality control of the drug and for the further studies on kidney toxicity metabolite.Methods Samples A.manshuriensis from different habitats were extracted by 75% methanol and analyzed by HPLC-DAD-ESI/MS,whose chromatographic fingerprints were established.Two ways to calculate the similarity were selected to compare the results by determining the common peaks.Results There were 30 main characteristic components in A.manshuriensis.The HPLC-DAD-ESI/MS fingerprint of the 30 common peaks was established preliminarily.The samples of A.manshuriensis from different habitats was found having a good similarity,and the range of similarities for 24 balches of A.manshuriensis were 0.871—0.998.Conclusion The method is reliable,accurate,and of good stability,and can be used for the quality control and variety identification of A.manshuriensis.

AIM:To check the antiproliferative and apoptosis-inducing effect of Compound Changtai Granule(Radix et Rhizoma ginseng,Semen coicis,Rhizoma curcumae,etc)(CCG) on human colon carcinoma cell line(SW480).METHODS:Methy thiazolyl tetrazolium chromatomerry was used to observe the influence of several concentration of CCG on SW480 cell proliferation cultured in vitro at the various times,and flow cytometry was applied to examining the change in SW480 cell proliferation cycle and apoptosis rate.RESULTS:CCG could successfully prevent SW480 cell from entering G0/G1 phase and G2/M phase,decrease cell numbers in S phase at the same time.CONCLUSION:CCG has an obvious dose-response relationship in the range of 3.51 to 7.81 mg/mL.