1.Effects of hypoxia on apoptosis of human lung adenocarcinoma A549 cell line by adriamycin
China Oncology 2001;0(02):-
Background and purpose:Hypoxic cells in tumors are of chemoresistant and anti-apoptoic.Our study was focused on the effects of hypoxia on expression of P-glycoprotein(P-gp) and cell apoptosis in human lung adenocarcinoma A549 cell line(wtP53),in order to explore the probable mechanism of hypoxia in apoptic tumor cells.Methods:A549 cell line(human lung adenocarcinoma) was cultured under hypoxic atmosphere(2% O_2) for 24 h,the expression of hypoxia inducible factor-1?、P-gp and P53 protein was detected by immunohistochemistry,after cells under hypoxic atmosphere were exposed to Adriamycin for 24 h,the cell survival rate was detected by MTT,and apoptosis of by flow cytometer.Results:① The expression of hypoxia inducible factor-1?、P-gp and P53 protein in hypoxic cells were higher than the expression in anoxia ,and correlation between the expression of HIF-1? and P-gp or P53 protein was observed(P
2.Advance in Acupuncture and Moxibustion in Spastic Paralysis After Stroke
Chinese Journal of Rehabilitation Theory and Practice 2006;12(9):791-792
In the past, treatment of acupuncture and moxibustion always were emphasized on improving the strength of muscle. In fact, spasticity is the key point. This paper summarized recent studies on therapeutic effect of acupuncture and moxibustion on spastic paralysis after stroke.
3.Editorial and introduction to Asian Journal of Andrology Prostate Cancer special issue.
Asian Journal of Andrology 2009;11(1):6-8
Antigens, Neoplasm
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urine
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Antineoplastic Agents
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therapeutic use
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Biomarkers, Tumor
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blood
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urine
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Biopsy, Fine-Needle
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Brachytherapy
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Global Health
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Humans
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Male
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Prostate
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diagnostic imaging
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pathology
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Prostate-Specific Antigen
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blood
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Prostatectomy
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Prostatic Neoplasms
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diagnosis
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epidemiology
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therapy
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Radiotherapy
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Taxoids
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therapeutic use
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Ultrasonography
5.Zonal differences in prostate diseases.
Chinese Medical Journal 2012;125(9):1523-1528
6.Learning and Memory Damage Induced by Formaldehyde and Protective Effect of N-acetyi-cysteine in Mice
Ya-Juan FENG ; Shu-Shu DING ; Jin-Xia ZHAI ;
Journal of Environment and Health 2007;0(08):-
Objective To explore the adverse effects of formaldehyde(FA)on learning and memory ability of mice and the antagonistic effect of N-acetyl-cysteine(NAC),an antioxidant.Methods Thirty-four ICR mice were randomly divided into four groups,the control(NS,n=8),treated with FA(15 mg/kg,n=9),treated with NAC(100 mg/kg,n=8),treated with FA(15 mg/kg) plus NAC(100 mg/kg,n=9),the treatment was conducted by intraperitoneal injection once a day for seven consecutive days.On the eighth day,the learning and memory ability were tested by using water labyrinth task for seven consecutive days.Results The mice in FA group behaved excited,restless and then turned to repose,moveless and clustering,but this phenomena was not seen in the other groups.There was no significant difference in the body weight of mice among groups.As for learning,latent period in the FA group [(27.15?2.66)s] was significantly longer than that in the control group [(15.83?2.82)s] and the FA+ NAC group[(14.98?2.66)s],and revealed statistical significance(P
9.Effect of Some Amino Acids in Gentamicin Production
China Biotechnology 2006;0(12):-
Gentamicin production at home and abroad was fermentation cycle long,production rate low,production costs high and short of competitiveness.Added a little quantity of lysine,glycine,tyrosine,methionine into the medium of gentamicin producing Micromonospora purpurea during inoculation could improve the metabolic capability of microbe,shorten fermentation cycle and increase production rate of gentamicin.The new method was successfully developed in shaking flask test and applied to 5L glass fermentor for one month.Average data of one month showed that the fermentation time per cycle was shortened about 40% and the yield of gentamicin per fermentor increased about 14% in comparison with the conventional technique.The production rate of gentamicin was increased 30% ~95%(according to the capability of strain).The quality of the end product gentamicin conformed to CP2000,BP2000 and USP26 pharmacopoeia.Therefore,the new method may reduce production costs of gentamicin remarkably,it shows strong competitiveness in the marketplace.
10.Several Correlative Ethical Issues on Community-Based Healthcare Service
Song PENG ; Shu-Xia HU ; Hong DING ;
Chinese Medical Ethics 1994;0(06):-
The community - based healthcare service is a main component of healthcare service system in China,and its development is affected by several factors including correlative ethical issues.In order to maintain a long - term and stable development of community - based healthcare service,great importance should be attached to some concerned ethical issues,such as the human - oriented service,intimacy of patients,the interests of disadvantaged group,etc.