Background and purpose:Hypoxic cells in tumors are of chemoresistant and anti-apoptoic.Our study was focused on the effects of hypoxia on expression of P-glycoprotein(P-gp) and cell apoptosis in human lung adenocarcinoma A549 cell line(wtP53),in order to explore the probable mechanism of hypoxia in apoptic tumor cells.Methods:A549 cell line(human lung adenocarcinoma) was cultured under hypoxic atmosphere(2% O_2) for 24 h,the expression of hypoxia inducible factor-1?、P-gp and P53 protein was detected by immunohistochemistry,after cells under hypoxic atmosphere were exposed to Adriamycin for 24 h,the cell survival rate was detected by MTT,and apoptosis of by flow cytometer.Results:① The expression of hypoxia inducible factor-1?、P-gp and P53 protein in hypoxic cells were higher than the expression in anoxia ,and correlation between the expression of HIF-1? and P-gp or P53 protein was observed(P

In the past, treatment of acupuncture and moxibustion always were emphasized on improving the strength of muscle. In fact, spasticity is the key point. This paper summarized recent studies on therapeutic effect of acupuncture and moxibustion on spastic paralysis after stroke.

Antigens, Neoplasm ; urine ; Antineoplastic Agents ; therapeutic use ; Biomarkers, Tumor ; blood ; urine ; Biopsy, Fine-Needle ; Brachytherapy ; Global Health ; Humans ; Male ; Prostate ; diagnostic imaging ; pathology ; Prostate-Specific Antigen ; blood ; Prostatectomy ; Prostatic Neoplasms ; diagnosis ; epidemiology ; therapy ; Radiotherapy ; Taxoids ; therapeutic use ; Ultrasonography

Asian Continental Ancestry Group ; Databases as Topic ; Humans ; Injury Severity Score ; Maxillofacial Injuries

Humans ; Male ; Prostate ; anatomy & histology ; pathology ; Prostatic Diseases ; pathology ; Prostatic Hyperplasia ; pathology

Objective To explore the adverse effects of formaldehyde(FA)on learning and memory ability of mice and the antagonistic effect of N-acetyl-cysteine(NAC),an antioxidant.Methods Thirty-four ICR mice were randomly divided into four groups,the control(NS,n=8),treated with FA(15 mg/kg,n=9),treated with NAC(100 mg/kg,n=8),treated with FA(15 mg/kg) plus NAC(100 mg/kg,n=9),the treatment was conducted by intraperitoneal injection once a day for seven consecutive days.On the eighth day,the learning and memory ability were tested by using water labyrinth task for seven consecutive days.Results The mice in FA group behaved excited,restless and then turned to repose,moveless and clustering,but this phenomena was not seen in the other groups.There was no significant difference in the body weight of mice among groups.As for learning,latent period in the FA group [(27.15?2.66)s] was significantly longer than that in the control group [(15.83?2.82)s] and the FA+ NAC group[(14.98?2.66)s],and revealed statistical significance(P

Humans ; Male ; Prostate-Specific Antigen ; blood ; Prostatectomy ; Prostatic Neoplasms ; blood ; metabolism ; radiotherapy ; surgery

Ethmoid Bone ; injuries ; Facial Bones ; injuries ; Humans ; Nasal Bone ; injuries ; Orbital Fractures ; surgery ; Osteogenesis, Distraction ; Skull Fractures ; surgery

Gentamicin production at home and abroad was fermentation cycle long,production rate low,production costs high and short of competitiveness.Added a little quantity of lysine,glycine,tyrosine,methionine into the medium of gentamicin producing Micromonospora purpurea during inoculation could improve the metabolic capability of microbe,shorten fermentation cycle and increase production rate of gentamicin.The new method was successfully developed in shaking flask test and applied to 5L glass fermentor for one month.Average data of one month showed that the fermentation time per cycle was shortened about 40% and the yield of gentamicin per fermentor increased about 14% in comparison with the conventional technique.The production rate of gentamicin was increased 30% ~95%(according to the capability of strain).The quality of the end product gentamicin conformed to CP2000,BP2000 and USP26 pharmacopoeia.Therefore,the new method may reduce production costs of gentamicin remarkably,it shows strong competitiveness in the marketplace.

The community - based healthcare service is a main component of healthcare service system in China,and its development is affected by several factors including correlative ethical issues.In order to maintain a long - term and stable development of community - based healthcare service,great importance should be attached to some concerned ethical issues,such as the human - oriented service,intimacy of patients,the interests of disadvantaged group,etc.