Objective To investigate the clinical characteristics and choices of management of neonatal testicular torsion (NTF).Methods Between January 2013 and December 2015,the clinical data of 7 cases of neonatal testicular torsion who were confirmed by surgery and pathological examination in the department of pediatric surgery of our hospital was retrospectively reviewed.Of the 7 NTTs (4 in right,2 in left and 1 in both sides),the median age and onset time of NNTs was 3 d (1-21 d) and 1 d (1-21 d),respectively.The presentation of NTTs included swelling of scrotum,scrotal mass and discoloration of scrotum.Features of ultrasonography included enlargement of testicular volume,echo heterogenicity or enhancement of testis,and decreased or disappeared testicular blood flow.Results All NTTs were performed by surgery and confirmed by pathological examination.In the operation,5 patients with extravaginal torsion (1 bilateral) and 2 with intravaginal torsion were detected.The degree of twisted testis was from 270° to 720°(average 438.8°) and resection rate for necrosis testis was 87.5％ (7/8).The median time of follow-up was 12 months (3-36 months),and none of testis underwent re-torsion and the findings of testicular ultrasonography was not abnormal.Conclusions The presentation of neonatal testicular torsion is non-specific.Once the scrotum shows discoloration,swelling,or enlargement of testis,testicular torsion is suspected.Urgent surgical exploration is recommended in order to make an effort to save the testis.

It is common in bacterial genomes of the integration of prophages. As an important participant of the vital movement of their hosts, prophages affect closely the biological properties of the hosts. Therefore, if we want to comprehend a bacterial genome fully, it is essential to recognize and understand accurately prophages in it. This article is a compendious review about the classification, distribution, identification, evolution of prophages and the interaction with their hosts.

Aortic Diseases ; Aortic Valve ; Aortic Valve Stenosis ; Calcinosis ; Humans ; Hyperlipoproteinemia Type II

Objective To investigate the apoptosis in gastric cancer induced by trichosanthin, and the relationship between this apoptosis and expression of bcl2. Methods In in vitro experiments, morphologic test and TUNEL staining method were used to quantitatively and qualitively detect the apoptosis status of gastric adenocarcinoma cell line SGC7901 before and after the trichosanthin treatment. Immunohistochemical staining method and Northern Blot hybridization were used for detecting expression status of apoptosisrelated genes bcl2, before and after trichosanthin treatment. Results When SGC7901 cells were treated with trichosanthin (0.1 ?g/ml, 36 h), they presented some typical apoptotic morphologic changes observed by fluorescent staining. These morphologic changes include nuclear condensation, nucleosomal fragments forming a lunate body under nuclear membrane, etc. When SGC7901 cells were treated with trichosanthin at the concentration 0.1 ?g/ml for 36 h,42 h and 48 h, respectively, TUNEL staining showed a significant increase of apoptotic index (AI), from 3.78%?1.11%, 3.98%?1.12%,3.85%?1.08%, respectively, to 11.30% ? 2.33%, 10.22% ?2.00%,11.18%?1.85%(P

Objective To investigate the effects of Oxytocin(OT) on hippocampal long-term potentiation(LTP) and FOS protein expression induced by peripheral stimulation.Methods Single stimulation pulses were delivered to the left sciatic nerves to evoke the field excitatory postsynaptic potentials (fEPSPs) in the right hippocampal CA1. Tetanic stimulation was used to induce hippocampal LTP. Different groups of rat were given NS,OT,Oxytocin antagonist-Atosiban + OT before tetanic stimulation,into lateral ventricle(LV) respectively. Expression of FOS protein was compared among the groups by histopathological and immunohistochemistry. Results Single stimulation evoked fEPSPs in hippocampal CA1,which average latency was (171.9?33.1)ms and average amplitude was (25.7?8.4)?V. Tetanic stimulation induced hippocampal LTP and increased the expression of FOS protein. Intracerebroventricular injection of OT inhibited hippocampal LTP and decreased the expression of FOS protein. This effect of OT was blocked by the pretreatment with Atosiban. Conclusion The results suggested that OT may play an inhibitory role in leaning and memory of rats and the effect is mediated by OT receptor.

Objective To observe the effect of Huangqi (Radix Astragali seu Hedysari) Injectio on the abnormal expression of aquaporin-1 (AQP-1) caused by glucose lactate peritoneal dialysis solution. Methods Different glucose concentration lactate peritoneal dialysis solutions and Huangqi Injectio given to the rats intraperitoneally. Eight weeks later, the ultrafiltration of peritoneal dialysis, peritoneum clearance, peritoneal structure, AQP-1 and its gene expression were observed. Results Compared with the control group, in the glucose and Huangqi groups, the ultrafiltration of peritoneal dialysis was remarkably decreased (P

OBJECTIVE:To analyze the chemical constituents of the essential oil extracted from Mosla chinensis seeds. METHODS: The essential oil was extracted from Mosla chinensis seeds by steam distillation method and identified by GC-MS, and its relative content was determined using normalization method. RESULTS: A total of 20 chromatographic peaks were detected and 17 chemical compounds were identified, which made up 99.06% of the total essential oil content. The major chemical constituents were carvacrol(57.01%), thymol(30.72%), thymol acetate(2.56%), carvacrol acetate(2.14%) and ?-caryophyllene(1.62%). CONCLUSION: The results in our study provides scientific basis for further exploitation and utilization of Mosla chinensis and serves as reference for establishing quality standard of Mosla chinensis herbal medicine.

Objective To study changes of DNA content in human spleen nuclei and seek an experimental method for estimation of the postmortem interval(PMI)using computerized image-analysis technique(CIAT).Methods Smear sections from spleen sampled were collected in 36 cadavers with known the accurate PMI respectively every hour within the first 36 hours after death,which were then fixed with cold Carony fixation.The smeared sections were stained by Feulgen-van's staining method.3 indices for spleen nucleic DNA including integral optical density(IOD),average optical density(AOD)and average gray(AG)were measured using the CIAT.Results IOD and AOD in the spleen nuclei declined regularly,whereas AG increased with the extension of PMIs in 36 hours.Conclusion There are definite relationships between the PMI and gray parameters(IOD、AOD and AG)representing the DAN content of nucleic DNA in the spleen in 36 hours after death,which may be used for estimation of PMI.

Objective To observe the polysomnographic manifestation of sleep disorder and the characteristics of sleep architecture in patients with PD.Methods 42 patients with PD and 40 normal controls underwent night polysomnography.The parameters of sleep architecture and progress in two groups and video-monitoring features were analyzed.Results According to PSG recordings,the incidence of difficulties in the initiation of sleep(73.8%),fragmented sleep(59.5%),excessive daytime sleepiness (46.1%)were respectively increased in PD patients group than that in controls(all P5)、 ESS were increased(P=0.022,0.000,0.007, 0.001,0.000,respectively).SOREMPs occurred in 6 patients(14.3%)in PD group,but didn't oecure in controls.In PD group REM without atonia(RWA)was demonstrated in 36 patients(85.7%),RBD in 19 patients;in control group,however,REM in 6 and RBD in 2 separately.The statistics analysis showed the incidence of RWA(85.7%)and RBD(45.2%)in PD group was significantly higher than that in control group(P