Objective To study the effect of simvastatin on high glucose induced vascular endothelial cells injury and explore its action mechanism .Methods Endothelial cells were devided into four group:control group(A) ,high glucose(33 .3 mmol/L)group (B) ,high glucose(33 .3 mmol/L)+simvastatin(1 .0 μmol/L)group(C) ,and high glucose(33 .3 mmol/L)+simvastatin(10 .0 μmol/L)group(D) .The inhibition of cells was detected by CCK-8 assay .The apoptosis was determined by TUNEL assay .The protein ex-pression was detected by Western blot analysis .Results The cells survival rates were (42 .5 ± 6 .4% ) ,(58 .6 ± 7 .8% ) ,and (71 .3 ± 11 .7% ) in B ,C ,and D group respectively .There was significant difference among the three groups(P<0 .05) .The per-cents of apoptosis were (1 .8 ± 0 .6% ) ,(45 .8 ± 8 .9% ) ,(22 .7 ± 6 .4% ) and (12 .6 ± 4 .2% ) in A ,B ,C and D group ,respectively . There was significant difference among the four groups(P<0 .05) .The protein expression of Bcl-2 was lower in B group(0 .13 ± 0 . 03)than that in A group(1 .02 ± 0 .16) .The Bcl-2 expression was higher in C group(0 .28 ± 0 .04)than that in B group ,and in D group(0 .68 ± 0 .11)than that in C group(P<0 .05 ,P<0 .01) .The gene expression of bax was higher in B group than that in A group .The bax expression was lower in C group than that in B group ,and the bax expression was lower in D group than that in C groups(P<0 .05 ,P<0 .01) .Conclusion Simvastatin can inhibit high glucose induced vascular endothelial cells apoptosis .Upregu-lation of Bcl-2 and downregulation of bax may be its action mechanism .

Objective:To study the effects of the secondary metabolites of Rhizotonia leguminicola on cyclophosphamide-induced immunoregulation in immunosuppressed mice.Methods:The immunosuppressed mouse model was established by cyclophosphamide(80 mg/kg) injected into abdominal cavity.The diluent of secondary metabolites of Rhizotonia leguminicola(SW) at the dosage of 8,16 and 32 mg/(kg?d) was intragastrically administered for 11 days respectively.Meanwhile,the normal control group and CTX model control group were established.Blood,thymus and spleen of the mice were collected respectively at 24 h after the last intragastric administration,and the immunological detection was also carried out.Results:CTX was injected constantly into the abdominal cavity at 80 mg/kg for 3 d,which leaded to immunosuppression of the mice.The groups which took SW at 8 and 16 mg/(kg?d) could increase the number of WBC of the mice.Cyclophosphamide induced a decrease of phagocytic activity of the peritoneal macrophages in the mice and a boost of hemolysin level of the peripheral blood,lymphocyte transformation of the spleens and the activity of NK cells,which were especially obvious in the group of SW at 16 mg/(kg?d).Conclusion:The secondary metabolites of Rhizotonia leguminicola have effects of recovery and reinforcement in cyclophosphamide-induced immunoregulation of immunosuppressed mice.

Objective To summarize radiofrequency cather ablation (RFCA)of tachycardias with right sid-ed accessory atrioventricular pathway in 7 patients with persistent left superior vena cava (PLSVC). Methods RF-CA for right sided accessory atrioventricular pathway in 7 patients with PLSVC was carried out . Coronary sinus ac-cess was performed through the left subclavian and the left femoral vein respectively. Swartz sheath was used during ablation if neccesary. Results There was no siginificant differences in fluoroscopy time between the left subclavian approach and the left femoral vein approach[(2.8 ± 0.6)min vs (3.2 ± 0.3)min, P>0.05)]. No recurrence of ac-cessory pathway conduction was found during a mean follow-up of (23 ± 11) months. The mean discharging time and fluoroscopy time were (312 ± 67)s and (17 ± 5)min respectively. Conclusions Although RFCA of right sided accessory atrioventricular pathway in patients with PLSVC shows specialities in cather manipulation , mapping and ablation, high successful results can be achieved by experienced physician.

Objective To evaluate the diagnostic value of spiral CT [CT arterial portography (CTAP) and CT hepatic arteriography(CTHA)] for hepatic carcinoma. Methods The CTAP and CTHA manifestations in 21 patients with hepatocelluar carcinoma were analyzed and compared with those in three-phase enhanced CT . Results The detection rate of three-phase enhanced CT was 72.4% , CTAP and CTHA was 96.1% and 88.2% respectively. The detection rate of combination CTAP and CTHA was 98.7% . They could reveal tumors of 0.5 cm in diameter . Both CTAP and CTHA gave false-positive findings , such as perfusion defects in 22.3% of CTAP and non-pathologic enhancement in 30.2% of CTHA .Conclusion With the use of spiral CT technique , the quantity of contrast material administrated in CTAP and CTHA can be considerably reduced . And the quality of CT images significantly improved . The detection rate of CTAP and CTHA is higher than that of three-phase enhanced CT . Simultaneous use of both procedures may decrease the false-positive rate.