1.Effeits of sotalol and amiodarone on patents with atrium filament trembles
Huijie ZHANG ; Shoutao CHENG ; Yueping GUO ; Yonghong ZHANG
Chinese Journal of Primary Medicine and Pharmacy 2008;15(9):1440-1441
Objective Curative effect of 2 drugs which present elinie used in atrium filament trembles,provides the elinieal experience.Methods Choice outpatient service atrial fibrillation trembles the patient 344 eases,plants stochastically according to sickness divides into two groups,takes the rope separately Sotalol and Amiedarone,the adjustment medication dosage treats and observes for 12 months,carries on the material summary.Results The rope Sotalol group and the Amiedarone group each is 172 people,the rope Sotalol group atrial fibrillation trembles transfers sinous rhythm 45 peoples,transfers rate 26.2%,recurs 5 patients,after transfers sinous rhythm maintenance rate 88.9% ; Amiodarone,group atrial fibrillation trembles transfers sinus rhythm 41 people,transfers rate 23.8%,recurs 2 patients,after transfers sinous rhythm maintenance rate 95.1% ; Conclusion Sotalol group treats atrial fibrillation trembles rate to be higher than the Amiedarone group,after transfers sinous rhythm maintenance rate Amiedarone group to be higher than Sotalol group.
2.Establishment of fluorescence immunochromatography detection for cytoskeleton-associated protein 4.
Lu ZHANG ; Yunlong WANG ; Yulin LI ; Jichuang WANG ; Yinyin YU ; Heng ZHANG ; Yiqing ZHANG ; Lei CHENG ; Shoutao ZHANG
Chinese Journal of Biotechnology 2020;36(6):1216-1222
A rapid and simple method to detect tumor markers in liver cancer was established by combining immunochromatography technique with fluorescent microsphere labeling. According to the principle of double antibody sandwich, the cytoskeleton-associated protein 4 (CKAP4) paired antibody was used as the labeled and coated antibody, and the goat anti-rabbit polyclonal antibody was used as the quality control line coated antibody in the preparation of the CKAP4 fluorescent immunochromatographic test strips. After the preparation, the test strips were evaluated on various performance indicators, such as linearity, precision and stability. The CKAP4 immunochromatographic strip prepared by time-resolved fluorescent microspheres had high sensitivity, and good specificity. Its precision was within 15%, recovery between 85% and 115%, and linear range between 25 and 1 000 pg/mL. The test strip could be kept stable at 37 °C for 20 days, and it correlated well with commercial ELISA kits. The CKAP4 fluorescence immunochromatography method can quantitatively detect the content of CKAP4 in serum. Furthermore, it is rapid, sensitive, simple, economical and single-person operation. This method has the potential of becoming a new method for the diagnosis and treatment of liver cancer.
Animals
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Antibodies
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metabolism
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Chromatography, Affinity
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Fluorescence
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Humans
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Liver Neoplasms
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diagnosis
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Membrane Proteins
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isolation & purification
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Molecular Diagnostic Techniques
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instrumentation
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methods
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Sensitivity and Specificity