AIM To investigate the effects of piroxicam on transplanted Sarcoma S180 and the expression of COX 2,VEGF,FGF 2 and microvessel density (MVD) in tumor tissue METHODS Kunming mice were randomizedly divided into control group, FT207 positive group and 5, 2 5, 1 mg?kg -1 piroxicam groups One day after inoculation of 0 2 ml S180 cell suspension, FT207 and piroxicam were given by gastric intubation for 9 days The inhibitory rate on S180 was calculated routinely The expression of COX 2,VEGF,FGF 2 and MVD was detected by immunohistochemistry RESULTS The growth of S180 was significantly inhibited by piroxicam at the doses of 5, 2 5, 1 mg?kg -1 with the inhibitory rate of 31 4%,40 7% and 34 9% respectively The expression of COX 2 in the tumor tissue was also inhibited by piroxicam. Accordingly the expression of VEGF,FGF 2 and MVD was markedly inhibited in dose dependent manner by piroxicam CONCLUSIONS The results suggest that piroxicam has inhibitory effects on S180,and it also decreases the expression of COX 2 in tumor tissue. There is a relation ship between the expression of COX 2 and angiogenesis related factor Antiangiogenesis may be another mechanism for piroxicam to exert its chemopreventive and treatment effects.

Objective To investigated the effects of IL -18 on middle ear allergic inflammation in rat model of OME .Methods Eighteen SD rats were randomly divided into three group :group A(control group ,n=12 ears) , group B (OME model group ,n=12 ears) ,group C (IL -18 injectiion group ,n=12 ears) .The rat model of OME was established by sensitizing with ovalbumin (OVA) and later challenging in tympanic bullae .Recombinant rat IL-18 (1 μg ,+0 .2 ml saline ,) were injected in group C at 1 ,2 ,7 ,8 ,15 ,16 day .At the same time sacle ,0 .2 ml sa-line ,instead of IL -18 ,were intraperitoneal injection in group A and B .The morphologic changes of the middle ear epithelial cells and inflammatory cells infiltration were observed under light microscope .The level of IFN -γand IL-4 in tympanic lavage fluid(TLF) were determined by ELISA .Results Pathological examination showed that middle ear mucosa inflammation and eosinophil infiltration in group C were no less severe than group B .The numbers of neutrophils in group C increased significantly compraring with group B (P<0 .05) .Numbers of eosinophils in group C were slightly increased comparing with group B (P>0 .05) ,while significantly greater than that in group A (P<0 .05) .ELISA showed that the level of IFN -γ in group C was stronger than that in group B and A (both P<0 .05) .As compared to the group A ,the expression of IL -4 in group B and group C were remarkably stronger (both P<0 .05) ,no significant difference was found between group B and group C (P>0 .05) .Addtionally ,there was no significant difference in the ratio of Th1 /Th2(IFN -γ/IL -4)between group B and group C (P>0 .05) . Conclusion IL -18 acts as an immune regulatory factors ,significantly increases Th1 cytokine IFN -γ.Although to some extent alleviate the OME rat middle ear Th1 /Th2 imbalance ,there is still excessive activation of Th cells . Th1 and Th2 cells factor are excessive for the secretion disorder of the immune response status .The OME rat mid-dle ear allergic inflammation has not been fundamentally alleviated ,the underlying mechamism should be further studied .

OBJECTIVETo probe the risk of colorectal polyp, colon and rectal carcinoma and the intake of NSAIDs.

METHODSCase-control study participants were from patients who underwent colonoscopy at different hospitals, the persons with the above disease was as cases, and those without the above diseases was as controls. Use of NSAIDs was assessed by interviewing the participants with a questionnaire which include a list of NSAIDs and related dietary and life style factors and family history.

RESULTSThere are 37 cases of colorectal polyp, 105 cases of colon carcinoma and 142 cases of rectal carcinoma and 66 controls. Adjusted for potential confounders, the risk of colorectal polyposis, colon carcinoma and rectal carcinoma were markedly reduced by NSAIDs. The OR values were 0.21 (95% CI 0.07-0.65, P = 0.007), 0.13 (95% CI 0.05-0.35, P < 0.001), 0.15 (95% CI 0.11-0.58, P < 0.001) respectively. The risk of the above diseases were also reduced markedly by aspirin, the OR values were 0.265 (95% CI 0.07-0.96, P = 0.044), 0.10 (95% CI 0.03-0.35, P < 0.001), 0.15 (95% CI 0.04-0.49, P = 0.002) respectively. The risk of colon carcinoma was also reduced by profen, with the OR being 0.11 (95% CI 0.02-0.64, P = 0.014).

CONCLUSIONSAspirin and other NSAIDs could reduced the risk of colorectal polyp, colon carcinoma and rectal carcinoma markedly. Aspirin was the most prospective chemopreventive agents for colorectal polyp, colon and rectal carcinoma for its capability of reducing the risk of cardio-cerebral vascular disease as well.

Adult ; Anti-Inflammatory Agents, Non-Steroidal ; therapeutic use ; Aspirin ; therapeutic use ; Colorectal Neoplasms ; prevention & control ; Female ; Humans ; Ibuprofen ; therapeutic use ; Logistic Models ; Male ; Middle Aged ; Piroxicam ; therapeutic use ; Polyps ; prevention & control ; Rectal Neoplasms ; prevention & control ; Risk Factors ; Sulindac ; therapeutic use ; Time Factors