The number, size, direction and position of the nutrient foramina were investigated in a total of 351 dry cervical vertebral arches of human adults. The position of the nutrient foramina is on the external and internal aspect of the arch and is more or less located in a fixed area, but the exact spot varies considerably. One foramen is more frequent on either aspect. The average diameters of the foramina on the external and internal aspect are 0.34 mm and0.26 mm respectively. The foramina on the external aspect are directed towards the pediele and those of the internal aspect backward.A total of 120 vertebral arches (except the atlas) in 20 fresh cadavers of different ages were used to demonstrate the nutrient arteries by dissection and translucent preparation. All nutrient arteries entering the foramina on the external aspectarise from the deep cervical artery and those of the internal aspect from spinal branches of the vertebral artery. After penetrating the arches, they divide into anterior and posterior branches. The former then subdivides into several branches leading to the pedicle, transverse process, upper and lower articular process, whereas the latter branches off into the lamina and spinal process. The course and distribution of the intraosseous arteries have close relation with the ossification of the arch.Small periosteal arteries penetrate the arch from the external aspect only and have not been found on the vertebral canal side except in the new borns.

The arterial supply of the human odontoid process was studied in 48 cadavers of different ages by dissection, clearing, and radiologic methods. The results were as follows:1. The odontoid process obtains its blood supply directly from the anterior and posterior ascending arteries arising from the vertebral artery and indirectly from the horizontal arteries arising from the ascending pharyngeal artery. Around the odontoid process these arteries form a peculiar anastomosis, the upper part of which is called the apical arch.2. There are two groups of nutrient arteries, the basal and the apical nutrient arteries, in the odontoid process. The basal nutrient arteries penetrate into the process at the base through the anterolateral aspect and the central part of the posterior aspect, mostly 1 branch (81.94?3.21％) from either side. The apical nutrient arteries enter it at the apex, and are usually divided into 2 branches (69.44?7.68％). The outer diameters of the basal nutrient arteries in the new horns, the children, and the adults are 0.09～0.15mm, 0.16～0.19mm, 0.24～0.29 mm, and those of the apical nutrient arteries are 0.03～0.06mm, 0.06～0.10mm, and 0.10～0.14 mm respectively.3. The pattern of arterial distribution within the odontoid process varies with the age. In subjects under twelve years the apex of the process has not yet ossified completely, and no anastomosis can be found between the arteries at the base and those at the apex. In adults ossification has been completed. The intraosseous arteries connect with each other and form an anastomosis network, which is most abundant at the base.

The arterial supply of human cervical vertebral bodies (C_3-C_7) was studied in 74 fresh cadavers of different ages by dissection and translucent preparation.1. The cervical vertebral bodies mainly obtain their blood supply from the vertebral artery, but the lower two (C_6-C_7) also receive their blood supply from the branches of the inferior thyroid, the deep cervical, the costocervical trunk, the highest intercostal and the subclavian artery. These arteries form a ladder-like anastomosis on the anterolateral surface of the vertebral bodies, and a rectangular or hexagonal anastomosis on the dorsal surface.2. The nutrient arteries enter the vertebral body from the anterolateral and dorsal aspects. They can be divided into the central branches which reach the center of the body and the peripheral ones which lie on the peripheral part of the body. Each of the central branches appears as a straight, unbranching stem. Their centrifugal terminals at the center of the body are arborized and extend to the upper, lower, left and right part of the body to supply the central core of the vertebral body which corresponds to the area of the nucleus pulposus. The peripheral branches, short and early branched, supply the peripheral part of the vertebral body which corresponds to the area of the annulus fibrous.3. The number of the central branches on the anterolateral aspect varies between 0-3 and that on the dorsal aspect is 0-2. The number of the peripheral branches on the anterolateral aspect is 2-13 and that on the dorsal aspect is 0-6. These branches anastomose with each other within the body of the vertebra. The end artery only appears in the developing cartilaginous regions of the body.

OBJECTIVE： To prepare Kangzhi lotion and observe its therapeutic effects on seborrheic dermatitis METHODS： To introduce the ingredients， preparation process and quality control of the lotion RESULTS:The total effective rate of the lotion on seborrheic dermatitis was 90 5％  CONCLUSION： The product is reliable in clinical effect and does not induce adverse reaction

OBJECTIVE The short-term efficacy of endoscopic adenoid hypothermic plasma ablation and its effect on Eustachian tube function. METHODS 75 patients with obstructive sleep apnea hypopnea syndrome who were treated in our hospital from March 2013 to March 2015 were divided into two groups according to the different operative methods: nasal power cutting group(30 cases) and low temperature plasma ablation group(45 cases), comparison of the two groups of patients with surgery time, intraoperative blood loss and follow-up after 6 months of clinical efficacy and Eustachian tube function. RESULTS The time of resection, operation time and intraoperative blood loss were significantly better in the low-temperature plasma ablation group than that in the rhinectomy group, the difference was statistically significant(P<0.05); The incidence of complications in the low-temperature plasma ablation group was significantly higher than that in the rhinectomy group, and the difference was significant(P<0.05); there was no significant difference between the two groups in the total effective rate and the postoperative disease-specific quality of life questionnaire(OSA-18) and there was no significant difference in the quality of life between the two groups(P>0.05). CONCLUSION The clinical efficacy of low-temperature plasma ablation is close to nasal dynamic cutting, but low-temperature plasma ablation surgery in the amount of bleeding was significantly less than the nasal excision. This surgical approach is minimally invasive and safe, but its postoperative secondary infection and secondary bleeding is high, so this need for further study.

Objective To explore the expression and significance of signal transducer and activator of transcription 3 (Stat 3), glucose transporter protein 1 (GluT-1) and proliferation cell nuclear antigen (PC NA) in lesions of condylomata acuminata (CA). Methods SP immunohistochemistry method was used to measure the expression of Stat 3, GluT-1 and PCNA in tissue samples from 40 cases of CA and 20 normal skin controls. Results The positivity rates of Stat 3, GluT-1 and PCNA were 85.0% (34/40), 87.5% (35/40) and 85.0%(34/40), respectively in CA tissue, 35.0% (7/20), 30.0% (6/20)and 55.0% (11/20),respectively in the control tissue; statistical difference was observed in these rates between the two groups (all P ＜ 0.05). The expression intensity of Stat 3, GluT-1 and PCNA was also higher in CA tissue than that in the controls. In addition, the expression intensity of PCNA was correlated with that of Stat 3 and GluT-1in CA tissue (both P＜ 0.05). Conclusions There is an overexpression of Star 3, GluT-1 and PCNA in CA tissue, and the overexpression of Stat 3 and GluT-1 may be associated with the over-proliferation of CA tissue.

Objective To evaluate the efficacy and safety of a 595-nm flashlamp-pumped pulsed dye laser in the treatment of patients at different ages with port-wine stains (PWS).Methods A retrospective review was performed in 1560 patients with PWS who had been treated with a 595-nm flashlamp-pumped pulsed dye laser.Treatment parameters were selected according to the age of and types of lesions in patients.Results The total response rate was 76.73％ (1197/1560) in all of the patients.Clinical efficacy of the flashlamppumped pulsed dye laser was closely correlated with patients'age (x2 =83.47,P ＜ 0.01) and types of lesions (x2 =46.30,P ＜ 0.01 ).There was a low incidence of adverse reactions which were well tolerable.Conclusion The 595-nm flashlamp-pumped pulsed dye laser is safe and effective for the treatment of PWS.

Objective:To investigate the influence of fetal liver AFT024 cells on the transfection efficiency of multidrug resistant gene 1(MDR1)and the in vitro expansion of CD34+ cells derived from umbilical cord blood.Methods:CD34+ cells were isolated from human umbilical cord blood by MACS CD34 Progenitor Cell Isolation Kit and co-cultured with AFT024 cells(AFT024 group)or cultured alone(control group)for 7 days.During the subsequent 14 days,retrovirus carrying MDR1 gene was supplemented twice a week to transfect CD34+ cells.On the 7th,14th and 21st day after culture,the number of total nucleated cells(TNC)was counted,the ratio of CD34+ cells was assayed by flow cytometry(FCM)and the number of CD34+ cells was calculated,and colony-forming cells(CFC)were counted by methylcellulose cultures.RT-PCR method was used to detect the level of MDR1 mRNA in the transfected cells.The expression and function of P-glycoprotein(P-gp)were evaluated by FCM assay and Rhodamine-123 efflux assay,respectively.The gene transfection efficiency was calculated by drug-resistant colony-forming cells assay.Results:(1)The MDR1 mRNA level in AFT024 group than that in control group.The gene transfection efficiency in AFT024 group was significantly higher than that in control group(46.0% vs 15.2%,P0.05).On the 14th day,the expansion fold of TNCs in control group was significantly higher than that in AFT024 group(P0.05).The expansion folds of CD34+ cells and CFCs in the AFT024 group were significantly higher than that of the control group(P

Objective:To identify causative genes for autosomal recessive woolly hair (ARWH) in a family.Methods:Clinical data were collected from two patients and other family members in a Chinese pedigree of Han nationality with ARWH. Peripheral blood samples were obtained from the two patients, their unaffected parents and 100 unrelated healthy individuals, and DNA was extracted from the blood samples. A next-generation skin-targeted sequencing panel was used to detect gene mutations in the patients, and Sanger sequencing was performed to verify the sequencing results. The function of protein encoded by the mutant gene was predicted.Results:Two missense mutations c.530T>G (p.Leu177Arg) and c.736T>A (p.Cys246Ser) were both identified in the LIPH gene of the two patients, which were inherited from their father and mother respectively. Neither of the two mutations was identified in the 100 unrelated healthy controls. Interspecies sequence alignment showed that leucine at amino acid position 177 and cysteine at amino acid position 246 of the protein encoded by the LIPH gene were highly evolutionarily conserved. As SIFT and Polyphen-2 softwares showed, the mutations c.530T>G (p.Leu177Arg) and c.736T>A (p.Cys246Ser) were both predicted to be detrimental variations.Conclusion:Two missense mutations c.530T>G (p.Leu177Arg) and c.736T>A (p.Cys246Ser) in the LIPH gene may contribute to the clinical phenotype of the two patients with ARWH in this family.

Objective:To evaluate the short-term clinical efficacy and safety of secukinumab in the treatment of moderate to severe plaque psoriasis.Methods:Thirty-six patients with moderate to severe psoriasis, who visited Department of Dermatology, Henan Provincial People′s Hospital from June 2019 to November 2019, received subcutaneous injection of secukinumab at a dose of 300 mg once a week for 5 weeks, followed by once every 4 weeks. Psoriasis area and severity index (PASI) was recorded at weeks 4, 8 and 12, and adverse drug reactions were observed during the treatment.Results:All the 36 patients received the treatment for at least 12 weeks. At week 4, 8 patients achieved PASI75, including 3 achieving PASI90 and 1 achieving PASI100; at week 8, 26 achieved PASI75, of which 16 achieved PASI90 and 4 achieved PASI100; at week 12, 32 achieved PASI75, of which 26 achieved PASI90 and 8 achieved PASI100. No patients had serious adverse drug reactions, such as severe infections or malignancies. One patient developed abdominal pain and distension after subcutaneous injection in the abdomen, and the symptoms disappeared after 3 days; 1 patient developed tonsillitis followed by eczema-like lesions on the original psoriaitc lesions; suppurative lymphadenitis occurred in the neck of another patient.Conclusion:Secukinumab is markedly effective in the treatment of moderate to severe plaque psoriasis with few adverse effects, and is a new treatment option for patients with moderate to severe plaque psoriasis.