AIM: To establish the quality standard of Huanglian Despensiing Granules (Rhizoma Coptidis). METHODS: TLC identifications of berberine hydrochloride, jateorrhizine hydrochloride and palmatine hydrochloride in Rhizoma Coptidis carried out. The contents of berberine hydrochloride and jateorrhizine hydrochloride were determined by means of HPLC. RESULTS: The linear ranges of berberine hydrochloride and jateorrhizine hydrochloride were 0.17076～0.838?g( r =0.99996), and 0.11796～0.5898?g( r =0.9959), respectively their average recoveries were 98.82 ( RSD =1.702) and 98.4 ( RSD =1.312), respectively. The characteristic spots of Rhizoma Coptidis could be detected by TLC. CONCLUSION: The methods reported in this paper are easy to operate, and can provide accurate and reproducible results, thus are able to control the quality of the Huanglian Despensing Granules.