Objective Discuss the treatment mechanism of Xiaofeng Xuanqiao Decoction on guinea pig with al ergic rhinitis. [Methods]Sixty guinea pigs were randomized into 6 groups: normal control group, al ergic rhinitis(AR) group, high-dosage, equivalent-dosage, low-dosage Xiaofeng Xuanqiao decoction group, and loratadine group. AR guinea pig model was successful y established by ovalbumin(OVA) chal enge method. Xiaofeng Xuanqiao decoction and loratadine were applied to intervene AR guinea pig. The serum levels of IgE and IL-4, IFN-γ, histamine were determined by ELISA methods. [Results]The expression levels of IgE and IL-4, histamine significantly increased,meanwhile the level of IFN-γsignificantly lower compared with the normal group(P<0.01). Compared with that in AR model group, the serum level of IgE and IL-4, histamine in high-dosage Xiaofeng Xuanqiao decoction group significantly decreased,but the level of IFN-γ increased(P<0.01); the levels of IgE and IL-4, histamine decreased in other medication groups, meanwhile the level of IFN-γ increased compared with the model group(P<0.05).[Conclusion]Xiaofeng Xuanqiao can promote the produce of IFN-γ, inhibit the formation of IL-4, promote the secretion of Th1/Th2 cytokines to restore balance, to relieve eosinophil activation, reduce the synthet-ic of IgE, reduce the release of histamine, in many ways to prevent al ergic rhinitis inflammation, to achieve therapeutic effect.

Prof. WANG Shou-chuan thought that children Tourette’s syndrome were due to wind and phlegm accumulation interior, imbalance between yin and yang. It is a manifestation excess include wind that is excess wind of liver wind and lung wind and deficiency wind due to yin deficiency, phlegm that is phlegm-fire and phlegm-turbidity; and root deficiency lying in the liver, spleen and kidney. He treated the patients with the main method of eliminating phlegm and extinguishing wind, according to the pathomechanism of wind and phlegm, accompanied with pacifying liver and purging fire, dispelling phlegm and calming mind, strengthening the spleen and nourishing the heart, nourishing the liver and tonifying the kidney. He produced the formula Fengning decoction, modif ication according to the symptoms of the patients. Its curative effect was remarkable and should be widely applied in clinic.

Study on the scientific essence of traditional Chinese medicine (TCM) syndrome is the key in TCM modernization. This paper showed new research ideas for TCM syndrome, which was based on the pediatric adenovirus pneumonia and its TCM treatment, metabonomics and its application in TCM syndrome research, in order to discuss its application value in the diagnosis and treatment of pediatric viral pneumonia. This method was mainly implemented through the metabonomics technology, which reflected TCM holism concept. The syndrome of phlegm-heat obstructing the lung, which was the most frequent syndrome in the pediatric viral pneumonia, was studied. Through the comparison with metabolites of healthy children, the biomarkers of syndrome of phlegm-heat obstructing the lung in pediatric viral pneumonia were identified.

Objective To study the effect of Qingfei oral liquid contained serum on the transforming growth factor-?1 (TGF-?1) and platelet-derived growth factor-BB (PDGF-BB) mRNA gene expression of human embryonic lung fibroblast cells infected by adenovirus (ADV) type 3I and 7b. Method TGF-?1 and PDGF-BB mRNA expression of human embryonic lung fibroblast cells infected by ADV type 3I and 7b were determined by in situ hybridization before and after treated with Qingfei oral liquid contained serum. Results ADV could up-regulate TGF-?1 and PDGF-BB mRNA of human embryonic lung fibroblast cells (P

Objective:To study the effect ofQingfei Oral Liquid medicated serum on the tumor necrosis factor-?(TNF-?) mRNA gene expression ofhuman embryonic lung fibroblasts induced by adenovirus Type3I, 7b.Methods:We determined the TNF-? mRNA ofADV-infected human embryonic lung fibroblasts before and after adding the medicated serum by in situ hybridization.Results:Adenovirus could up-regulate the TNF-?mRNA ofhuman embryonic lung fibroblasts(P

Objective: To probe the curative effect evaluation method of traditional Chinese medicine in treating pediatrics virus pneumonia.Methods: On the basis of the outcome of analysis made on the preceding three turns of experts' questonnaires,the fourth turn of questionnaires was prepared to investigate the curative effect evaluation method of traditional Chinese medicine in treating pediatrics viral pneumonia.They were distributed to 30 experts in this field all over the country.The questionnaires were analyzed with the method of Delphi Measurement.Results: Chest X ray,cough,temperature,lung auscultation and so on were very important in disease curative effect evaluation,the ratio of full score higher than 0.95 and the coefficient of variation less than 0.24;cough,lung auscultation,Chest X ray and so on were main components in the syndrome and the ratio of full score and the coefficient of variation were respectively more than 0.90 and less than 0.34;dyspnea with rapid and short breath,lung auscultation and Chest X ray were main final result index and the ratio of full score and the coefficient of variation was respectively 1 and 0.Conclusion: Chest X ray,symptoms,temperature and lung auscultation were very important in disease curative effect evaluation;cough,lung auscultation and Chest X ray were main components in the syndrome;dyspnea with rapid and short breath,lung auscultation and Chest X ray were main final result index.

For example of children virus pneumonia, we put forward that clinical research should be carried out by review article analysis, experts’opinions integrated and the requests of evidence-based medicine. On the base of systemic evaluation of the sensitivity, reliability, validity and relation among them of all indicators for effect evaluation on virus pneumonia, we will establish the effect evaluation system of TCM in the treatment of virus pneumonia including disease and syndrome effect evaluation, complication and combining drug,using rate evaluation, safety evaluation and health economics evaluation.

Objective:To study the application of direct immunofluorescence in detecting RSV antigen in children respiratory syncytial virus(RSV)pneumonia.Methods:Multi-center,block randomization,parallel control clinic tests were adopted.To use Qingkailing Injection and Ertongqingfei Oral Liquid as test group and ribavirin injection and Compound Guaiacol Potassium Sulfonale Oral Solution as control group for treating accumulation of phlegm-heat in lung syndrome in children RSV pneumonia.Direct immuno uorescence was used to detect RSV turning to negative in different period in treatment time.Results:The time of turning to negative of test group was earlier than the control group's.In the case of 208 include 108 in the test group and 98 in the control group,it had statistic significance(P0.05).Conclusion:Detection of RSV antigen by direct immuno uorescence is simple,stable,sensitive and special.It is better in choosing clinical case and tracing clinical effect and should be widely applied in clinic.

This article explained that the evidence grading system occupied an important position in the evidence-based clinical practice guidelines. Through the comparative analysis on characteristics of clinical evidences between traditional Chinese medicine (TCM) and modern medicine, different possible establishment ideas of evidence grading system in TCM evidence-based clinical practice guidelines were proposed . The design for evidence grading standard of Chinese medical literature which reflected the academic characteristics of TCM was suggested.

Objective: To study the effect of Jinxin Oral Liqiud medicated serum on RS virus virus (RSV) infected cell apoptosis and the regulation gene as Bcl-2, Bax on the different point in early time. Methods: Cell culture, serum pharmacology and Annexin V/PI technique combining with flow cytometry were used to detect cell apoptosis, Bcl-2 and Bax expression. Results: The total apoptosis rates of human embryonic lung fibroblast in JOL group in early time of 12h and 24h were significantly higher than that in RSV infected group (P