Oviduct,egg and protein contents of 1~3 years old Rana chensinensis were studied. Results showed thatthe oviduct content and the absolute value of eggs were significantly higher in 3-year o1d frogs,While the rela-tive va1ue of eggs was highest in 2 years old frogs- Crude protein content varied in different aged group as wellas in different parts of the body,with eggs containing the highest amount. Protein in oviduct was comparative-ly consistant. Protein content in deviscerized body was rather high with no significant difference between maleand female. Frogs before and after hibernation have essentially similar oviduct and protein content,but eggcontent were significantly higher after hibernation. Protein content in eggs was not notab1y changed.

Objective:To investigate the effect of IL-7 on differentiation of thymic T lymphocytes and dendritic cells' differentiation in vitro.Methods:Thymic lobes were apspectically removed from 15-day-old or 16-day-old mouse fetueses and were cultured by the way of fetal thymus organ cultures (FTOC) in vitro.Lobes were cultured in the dishes with or without Interleukin-7(IL-7),then collected the cultured cells respectively.The expression of CD4,CD8,CD11c,B220,Ia,and CD11b was detected by flow cytometry,and the morphology was observed under the ligt microscope.The proliferation of these cells was determined by cell counting.The cultured cells were used as stimulators for allogeneic T cells,whose proliferation were detected later by MTT method.Results:The result of cell counting indicated that the total amount of the thymocytes was decresed obviously after being cultured with IL-7,while the proportion of CD4~+ CD8~+ thymocytes decreased,the proportion of CD4~- CD8~-,and CD4~- CD8~+ thymocytes increased,and no much difference in the amount of CD4~+ CD8~- thymocytes was found.In addition,the amount of B lymphocytes,natural killer cells,and dendritic cells increased in different degree.Conclusion:IL-7 participates in differentiation and development of thymic T lymphocytes and dendritic cells.