Objective To analyze the effect of health education on recurrence of acute pancreatitis in discharged patients. Methods All patients with acute pancreatitis accepted health education during the first hospitalization.92 patients were randomly allocated to the trial group and the control group.The trial group was kept on being treated with health education for 18 months after discharged form the hospital but the control group didn' t.Results The recurrence rate of trial group was 8.50% but the control group was 24.44%(P

OBJECTIVETo characterize the role of Smads proteins in alpha 2 (I) collagen (COL1A2) gene expression induced by bone morphogenetic protein-2 (BMP-2) in odontoblast cell line MDPC-23.

METHODSEndogenous Smad protein expression was determined by immunocytochemistry. Smads function and their role in COL1A2 gene expression were investigated in cotransfection experiments using promoter-luciferase reporter gene construct.

RESULTSMDPC-23 cells expressed Smad1, Smad5 and Smad6. BMP-2 promoted the activation of COL1A2 promoter reporter construct. Transient overexpression of Smad1 or Smad5 was enhanced, while overexpression of Smad6 inhibited BMP-2-induced COL1A2 promoter activity. BMP-2 inducibility could be blocked by overexpression of Smad1 or Smad5 dominant negative mutant.

CONCLUSIONSSmad signaling is functioning and appears to be involved in BMP-2-induced COL1A2 collagen transcription in MDPC-23. Smad signaling may play an important role in odontoblast differentiation and dentin extracellular matrix formation mediated by BMP-2.

Animals ; Bone Morphogenetic Protein 2 ; Bone Morphogenetic Proteins ; genetics ; Cell Line ; Collagen ; genetics ; Collagen Type I ; Mice ; Odontoblasts ; cytology ; metabolism ; Smad Proteins ; physiology ; Transforming Growth Factor beta ; genetics

OBJECTIVETo investigate the role of c-Jun and c-Fos as transcriptional factors in regulation of dentin sialophosphoprotein (DSPP) gene by a promoter-luciferase reporter gene construct in odontoblast cell line MDPC-23.

METHODSEndogenous c-Jun or c-Fos protein was determined by immunocytochemistry. The role of c-Jun or c-Fos in transcription of DSPP was investigated in co-transfection experiments using promoter-luciferase reporter gene construct containing the sequence between -791 bp and +54 bp of mouse DSPP gene.

RESULTSc-Jun and c-Fos was expressed by MDPC-23 cells, and located in the nucleus of MDPC-23 cells. Overexpression of c-Jun or c-Fos significantly inhibited luciferase activity of DSPP promoter.

CONCLUSIONThese findings suggest c-Jun and c-Fos down-regulated the transcription of DSPP gene as a transcriptional factor in odontoblast.

Animals ; Cell Line ; Extracellular Matrix Proteins ; Gene Expression Regulation ; Mice ; Odontoblasts ; Phosphoproteins ; Promoter Regions, Genetic ; Sialoglycoproteins ; Transfection

BACKGROUND:The outcome of cardiopulmonary resuscitation (CPR) may depend on a variety of factors related to patient status or resuscitation management. To evaluate the factors influencing the outcome of CPR after cardiac arrest (CA) will be conducive to improve the effectiveness of resuscitation. Therefore, a study was designed to assess these factors in the emergency department (ED) of a city hospital.METHODS:A CPR registry conforming to the Utstein-style template was conducted in the ED of the First Affiliated Hospital of Wenzhou Medical College from January 2005 to December 2011. The outcomes of CPR were compared in various factors groups. The primary outcomes were rated to return of spontaneous circulation (ROSC), 24-hour survival, survival to discharge and discharge with favorable neurological outcomes. Univariate analysis and multivariable logistic regression analysis were performed to evaluate factors associated with survival.RESULTS:A total of 725 patients were analyzed in the study. Of these patients, 187 (25.8％) had ROSC, 100 (13.8％) survived for 24 hours, 48 (6.6％) survived to discharge, and 23 (3.2％) survived to discharge with favorable neurologic outcomes. A logistic regression analysis demonstrated that the independent predictors of ROSC included traumatic etiology, first monitored rhythms, CPR duration, and total adrenaline dose. The independent predictors of 24-hour survival included traumatic etiology, cardiac etiology, first monitored rhythm and CPR duration. Previous status, cardiac etiology, first monitored rhythms and CPR duration were included in independent predictors of survival to discharge and neurologically favorable survival to discharge.CONCLUSIONS:Shockable rhythms, CPR duration ≤15 minutes and total adrenaline dose ≤5 mg were favorable predictors of ROSC, whereas traumatic etiology was unfavorable. Cardiac etiology, shockable rhythms and CPR duration ≤15 minutes were favorable predictors of 24-hour survival, whereas traumatic etiology was unfavorable. Cardiac etiology, shockable rhythms, CPR duration ≤15 minutes were favorable predictors of survival to discharge and neurologically favorable survival to discharge, but previous terminal illness or multiple organ failure (MOF) was unfavorable.

Objective To observe the alterations of each menstrual factor in ovarian aging .Methods The data were obtained from one-year observation of a prospective cohort involving 323 healthy women in the age of 30-54 years in a community in Beijing .Menstrual diary was kept and serum level of follicle-stimulating hor-mone ( FSH) was tested .The participants were divided into groups based on age , FSH level , and stages of repro-ductive aging.Alterations of length of menstrual cycle (LMC), length of menstrual period , and menstrual volume were analyzed in each group .Results Age:the mean LMC extended after the age of 40 years, obviously after 48, and peaked with (67.04 ±36.77) days in≥52-year-old group.The mean length of menstrual period did not vary much with age .However , the variation of mean length of menstrual period gradually increased with age after 44 years old, peaked in 50-to 51-year-old group.The menstrual volume scores were approximately stable between 38 and 47 years of age , with a peak of (45.06 ±93.54) in48 -to 49-year-old group and a minimum of (15.87 ± 13.81) in≥52-year-old group.FSH level:LMC increased with elevation of FSH levels .There was no signifi-cant difference in the mean length of menstrual period among FSH level groups , but the variation of length of menstrual period was maximum in 2040U /Lgroup .Stages of reproductive aging:the length of menstrual period and menstrual volume scores among each stage showed no statistically significant difference ( P>0.05 ) .Co nclusions The LMC altered significant-ly in ovarian aging , which could be used as staging criteria of reproductive aging .The length of menstrual period and menstrual volume are also showing some characteristic changes , possibly of some clinical values .

OBJECTIVETo identify single nucleotide polymorphisms (SNP) in the regulatory and coding regions of human kynureninase (KYNU) gene in a hypertensive candidate chromosomal region 2q14-q23 of Han Chinese, and to investigate the relationship between polymorphisms in KYNU and essential hypertension.

METHODSThe SNPs in the promoter region and exons of the KYNU gene were detected by direct DNA sequencing. Genotyping of the nonsynonymous Lys412Glu (A/G) polymorphism was performed by DHPLC technology in 456 hypertensive patients and 430 normal controls.

RESULTSSixteen SNP were identified in the KYNU gene, including 6 in the regulatory region and 2 in the coding region (both of them lead to substitution of amino acid). Significant differences between hypertensive patients and normal controls were observed for the distribution of alleles (chi(2) = 6.693, P = 0.035) and genotypes (chi(2) = 4.188, P = 0.041) of the Lys412Glu polymorphism in all subjects, and for the distribution of alleles in the subgroup of men (chi(2) = 4.424, P = 0.035).

CONCLUSIONThe Lys412Glu polymorphism of the KYNU gene in a hypertensive candidate chromosomal region is associated with essential hypertension in Han Chinese.

Adult ; Alleles ; Asian Continental Ancestry Group ; genetics ; Exons ; Female ; Gene Frequency ; Humans ; Hydrolases ; genetics ; Hypertension ; epidemiology ; genetics ; Male ; Middle Aged ; Polymorphism, Single Nucleotide ; Promoter Regions, Genetic

OBJECTIVETo observe the effect of Schistosoma japonicum cysteine protease inhibitor (rSjCystatin) for treatment of lipopolysaccharide (LPS)-induced sepsis in mice.

METHODSAfter a week of adaptive feeding, 54 BALB/c mice were randomly divided into normal control group (group A), sepsis group (group B), and rSjCystatin intervention group (group C). The mice in group A received an intraperitoneal injection of PBS (100 µL), and those in groups B and C were injected with PBS (100 µL) containing LPS (10 mg/kg); the mice in group C were also intraperitoneally injected with 25 µg sjCystatin in 100 µL PBS 30 min after LPS injection. From each group, 10 mice were randomly selected 24 h after PBS or LPS injection for detecting serum levels of TNF-α, IL-6, and IL-10 using ELISA and the levels of ALT, AST, BUN, and Cr using automatic biochemical analyzer; the pathological changes in the liver, lung and kidney were observed with HE staining. The remaining 8 mice in each group were used for observing the changes in the general condition and the 72-h survival.

RESULTSThe 72-h survival rates of the mice was 100% in group A, 0 in group B, and 36% in group C, showing a significant difference among the 3 groups (P<0.05). Compared with those in group A, the mice in group B exhibited obvious liver, lung, and renal pathologies with increased levels of ALT, AST, BUN, Cr, IL-6, and TNF-α (P<0.05). Treatment with sjCystatin significantly lessened LPS-induced organ pathologies, lowered the levels of liver and renal functional indexes and the pro-inflammatory cytokines, and increased the serum level of IL-10 in the mice (P<0.05).

CONCLUSIONSjCystatin can produce a significant therapeutic effect on sepsis induced by LPS in mice.

Objeetive To evaluate the impact of viable myocardium assessed by 99Tcm-MIBI SPECT and 18F-flourodeoxyglucose(FDG) PET imaging in patients with left ventricular aneurysm(LNA) underwent revascularization (RVS).Methods Forty-six consecutive patients with LVA (mean LVEF 36%±7%),underwent 99Tcm-sestamibi SPECT and 18F-FDG PET examinations and received RVS therap,were followed-up for a mean period of 80±27 months.Viable myocardium in anenrysm was defined as perfusionmetabolism mismatch score (MMS) ≥2.0.Patients were divided into four groups by aneurysm viability and aneurysmectomy.Group A1 (n=8):viability-;Group A2 (n=15):viability-,aneurysmectomy;Group B1 (n=10):viability+;and Group B2 (n=13):viability+,aneurysmectomy.Results The cardiac event rates during follow up were similar among groups [A1(25%,2/8),B1(40%,6/15),A2(20%,2/10) and B2(31%,4/13:P＞0.05].After revascularization,LVEF was impmved (＞10%) in groups A2,B1 and B2(P＜0.05).Multivariate logistic regression analysis showed that LV-MMS(OR=2.34,95% CI 1.08-5.06,P＜0.05).distal vessel disease(OR=0.008,95% CI 0.001-0.560,P＜0.05) and nonaneurysm perfusion score (OR=0.24.95% CI 0.07-0.85.P＜0.05) were significantly associated with the improvement of LVEF after revascularization.Conclusions Long term cardiac events rate post revascularization was not affected by viable myocardium or aneurysmectomy in LVA patients.Viable myocardium in LVA patients was associated with better LVEF improvement after revascularization.

Objective: To evaluate the correlation between ultrasound features of papillary thyroid carcinoma (PTC) and lymph node metastasis by preoperative ultrasound elemental observation of thyroid nodules. Methods: Three hundred and seventy-six patients who underwent primary thyroid surgery and confirmed by ultrasound and pathological data as single-focal PTC from Jannary to December 2017 in Sir Run Run Shaw Hospital of Zhejiang Univbersity College of Medicine were retrospectively analyzed. According to the presence or absence of lymph node metastasis, they were divided into central and lateral lymph node metastasis group and non-metastasis group. Independent risk factors for central lymph node metastasis (CLNM) and lateral lymph node metastasis (LLNM) were analyzed by χ² test and multivariate Logistic regression. Results: Multivariate analysis showed that the posterior margin of the cancer was <0.25 cm from the posterior wall of the thyroid gland as an independent risk factor for CLNM (P=0.025). Compared with the tumor volume ≤0.38 cm³, the cancer volume >0.38 cm³ (P=0.000), was more prone to CLNM. And multivariate analysis showed that the anterior margin of the cancer was <0.17 cm (P=0.006) from the anterior thyroid capsule and the inner wall of the foci was <0.26 cm (P=0.014) as independent risk factors for LLNM. Compared with the maximum diameter of the tumor lesion ≤1 cm, the maximum diameter >2 cm (P=0.001) group was more prone to LLNM. Compared with the tumor volume ≤0.38 cm³, the tumor volume >0.38 cm³ (P=0.000) was more prone to LLNM. Conclusions The larger volume of single focal PTC carcinoma and the closer to the posterior thyroid capsule are independent risk factors for CLNM. The larger volume and diameter of single focal PTC, and the closer to the anterior and medial wall capsule are independent risk factors for LLNM.