Myringoplasty using exogenous collagen membrane was carried out in 10 rabbits (20 ears) after complete removal of drumhead. The healing results were observed at 10, 20, 40, and 60 d, after operation. The 19 ears were transplanted successfully. Meanwhile, observational comparisons were made of the histology and ultrastructural morphology of the rabbits tympanic membrane before and after transplantation. The results confirmed that the graft has a good property of biocompatibility.and that it is of great practical value in the transplantation of tympanic membrane.