Objective　The age-related changes of calcitonin gene-related peptide （CGRP）immunoreactive nerve fibers in dental pulp-dentine complex were studied to explore the clinical signs of dental disease and their mechanism of aging.　Methods　Human premolars were collected and divided into 4 groups according to age. All samples were embedded in paraffin and cut into slices. These slices were treated by immunohistochemical reaction of CGRP. The image analysis was used for quantitative analysis and SAS software was used for statistics. 　Results　By aging, the bundle of CGRP-immunoreactive nerve became thin gradually, the numbers of CGRP-immunoreactive nerve branch decreased, the staining density of CGRP-immunoreactive nerve also descended and the distribution of CGRP-immunoreactive nerve fibers into predine was shorter. Image analysis showed that the intergral optical density, volume density, length density and line length of CGRP-immunoreactive nerve were all decreased by aging. The differences in volume density, length density and line length of CGRP-immunoreactive nerve were significant among different groups, but this significance was not found in parameter of integral optical density of CGRP-immunoreactive nerve.　Conclusions　The numbers, branches and extent of distribution of CGRP-immunoreactive nerve fibers decreased by age. The sensitivity of CGRP-immunoreactive nerve may also be lower by age.

Objective To establish a monitoring and evaluation indicator framework in the context of malaria elimination in pilot areas in China,so as to further assess the malaria elimination work in the future. Methods Based on the consultancy, and according to the government documents such as Action Plan for Malaria Elimination in China (2010-2020),Technical Guidance for Malaria Elimination,Programme Evaluation on Malaria Elimination,the indicators were set up at both provincial and county levels and their application was conducted in Yunnan and Anhui provinces to assess the accountability,accessibility and applicability. Results An indicator framework was built up including four key components,which were input,process, output and impact indicators,in which 95 were provincial indicators and 98 were county indicators. The completion rate of the indicators of Yunnan and Anhui provinces were 94.7%(90/95)and 95.8%(91/95)respectively;and the completion rate of in?dicators of Tengchong and Feidong counties were 93.9%(92/98)and 92.9%(91/98)respectively. Conclusion The estab?lished indicator framework is reliable and applicable,which could effectively help to track the malaria elimination progress and identify gaps.

Objective To provide the reference for schistosomiasis control through analyzing the changes of endemic status of schistosomiasis in China in recent years. Methods The annual data of schistosomiasis prevention and control from 2002 to 2012 were collected, and the descriptive analysis was conducted to describe the changes of schistosomiasis endemic status. Re?sults Sichuan, Yunan and Jiangxi provinces reached the criteria of transmission controlled from 2002 to 2010 while Hubei, Hu?nan, Jiangxi and Anhui provinces reached the criteria of infection controlled in 2008. The number of counties where the trans?mission of schistosomiasis was controlled decreased from 110 in 2002 to 80 in 2010. The numbers of estimated schistosomiasis cases and reported acute cases fell from 810.4 thousands and 913 in 2002 to 325.8 thousands and 43 in 2010, respectively, and they were reduced by 59.79%and 95.40%respectively. The number of infected bovine went down from 23 199 in 2002 to 7 173 in 2010, with a fall of 69.03%. However, the Oncomelania hupensis snails breeding areas kept in 3.7 to 3.8 billion m2 and among which, 125 million m2 areas were newly discovered in non?endemic areas during the nine years. In 2010, 90%of schistosomiasis cases, acute cases, infected bovine, and snail breeding areas were concentrated in 5 provinces, especially in the Dongting Lake and Poyang Lake areas and the regions along the Yangtze River. Conclusions The epidemic of schistosomiasis decreased sig?nificantly from 2002 to 2010. The lake regions are the key and tough areas for schistosomiasis prevention and control. To consoli?date the achievements, the surveillance and case management need to be strengthened as the extension of snail breeding areas and acute cases reported from other places becomes common.

Objective To assess the theoretical knowledge and practical skills of parasitic diseases among technicians from disease control and prevention institutions. Methods The Assessment on National Parasitic Disease Control and Prevention Techniques was organized in September,2015. Together,124 subjects from disease control and prevention institutions at prov-ince,prefecture or county levels in 31 provinces joined the assessment. A database was built consisting of subjects'basic infor-mation and assessment scores. Statistical analysis was used to analyze the scores by gender,age,professional title,institutions and places of participants. Results The average total score of all the subjects was 123.3,with a passing rate of 57.3%. The av-erage scores of male subjects(48 subjects)and female subjects(76 subjects)were 125.9 and 121.7 respectively;the average scores of the subjects aged under 30 years(57 subjects),between 30 and 40 years(61 subjects)and above 40 years(6 sub-jects)were 119.6,128.1 and 111.2 respectively;the average scores of persons with junior(94 subjects),intermediate(28 sub-jects)and senior(2 subjects)professional titles were 119.2,135.9 and 140.5 respectively. The average theoretical assessment score of all the subjects was 61.9,with a passing rate of 62.9%. The average practical skill assessment score of all the subjects was 61.4,with a passing rate of 58.1%. Conclusions The theoretical assessment results range widely. The theoretical knowl-edge results of technicians from disease control and prevention institutions are low in general. Therefore ,the specific training based on daily work needs to be enhanced.

ObjectiveTo observe the rehabilitative effect of patients with shoulder-hand syndrome after stroke by manipulation treatment. MethodsThe patients with shoulder-hand syndrome were randomly divided into two groups, manipulation group (180cases) and control group (128 cases). Patients in the manipulation group were regularly given a passive quantitative movement on shoulder, elbow and hand joints,while patients in the control group were irregularly given a passive movement or ordered to perform an autonomic movement. The signs and symptoms of patients in these two groups were not much different. The rehabilitative effects were compared 3 months later. ResultsSigns and symptoms in the manipulation groups improved much better than that of the control group. Conclusions The manipulation treatment for the post-stroke patients with shoulder-hand syndrome is the method that is simple, effective and easy to perform.