Objective To investigate the effects of matrix metalloproteinase-3 (MMP-3) in the development of neuropathic pain (NP) in rats.Methods One hundred and eighty male Wistar rats weighing 200-250 g were randomly divided into 4 groups (n =45 each):sham operation group (S group),NP group,MMP-3-siRNA group (Msi group) and MMP-3-siRNA negative control group (NC group).NP was induced by ligation of L5 spinal nerve (SNL).Lentivirus with MMP-3-siRNA was injected intrathecally after SNL in group Msi.Lentivirus with NC-siRNA was injected intrathecally after SNL in group NC.The mechanical pain threshold was measured at day 7,14 and 21 after SNL.The rats were then sacrificed after the last measurement of the mechanical pain threshold.The lumbar segment of the spinal cord (L4-6) was removed for determination of the expression of MMP-3 and TNF-a protein and mRNA by RT-PCR and Western blot.Results The mechanical pain threshold was significantly decreased and the expression of MMP-3 and TNF-α protein and mRNA was up-regulated at each time point in groups NP and NC as compared with group S (P ＜ 0.05).The mechanical pain threshold was significantly higher and the expression of MMP-3 and TNF-α protein and mRNA was lower at each time point in Msi group than in NP group (P ＜ 0.05).Conclusion Up-regulation of the expression of MMP-3 activates microglia and induces the release of TNF-α,resulting in NP in rats.

Objective To explore the clinical manifestations and radiological features of cystic fibrosis (CF) in children.Methods The clinical and radiographic data of 5 CF patients were retrospectively analyzed.Results Among the 5 cases,there are 3 males and 2 females,aging from 2 to 13 years old (median age 6).Four of the five cases had complaints of repeated productive cough with or without fever and short breath.Pseudomonas aeruginosa was positive in sputum culture of three cases.Chest CT showed pneumonia and bronchiectasis with peribronchial thickening and mucus plugging.Paranasal CT showed frontal sinus agenesis and sinusitis with sticky secretion.The other one of the 5 cases had a complaint of abnormal hepatic function.The abdominal MRI showed liver cirrhosis and high signal intensity in the periportal area on T1-weighted imaging.Chest CT showed air trapping from small airways obstruction and bronchiectasis with sputum plugging.Five recurrent and two novel CFTR mutations were identified in all of the 5 cases.Conclusions The radiographic findings of CF are characteristic,and of great significance to the clinical diagnosis of CF.The gene mutations of CF in Chinese are different from those in Caucasians.

By searching for the Canadian Licensed Natural Health Products Database, (LNHPD), this paper analyzed the characteristics and current status of 92 Chinese patent medicines successfully registered and listed in Canada, and found that the enterprises of successfully registered enterprises are mainly located in areas with better development condition of Traditional Chinese Medicine (TCM) such as Beijing, Guangdong and Tianjin; The successfully registered Chinese patent medicines include 64 kinds of single medicine or medicine with single active ingredient (69.6%) and 28 kinds of compound medicine (30.4%), the forms of the dosage are mainly tablets and capsules, which have the characteristics of accuracy in dosage and stable physicochemical properties. There are also granules, solutions, powders and other dosage forms, which can be preserved for a long time and have low requirements on technic and environment. These Chinese patent medicines are mainly used to treat respiratory and circulatory system diseases, some are used to treat urogenital and digestive system diseases, and few are used to treat difficuilt diseases like tumors, diabetes. There are some other health care products. It is suggested to strengthen the connection between domestic standards of TCM registration and international standards, and promote the scientific and technological capacity of relevant enterprises, and encourage enterprises to strengthen international registration of advantageous products, so as to accelerate the speed of international development of TCM in China.

CRISPR, as an emerging gene editing technology, has been widely used in multiple fields due to its convenient operation, less cost, high efficiency and precision. This robust and effective device has revolutionized the development of biomedical research at an unexpected speed in recent years. The development of intelligent and precise CRISPR delivery strategies in a controllable and safe manner is the prerequisite for translational clinical medicine in gene therapy field. In this review, the therapeutic application of CRISPR delivery and the translational potential of gene editing was firstly discussed. Critical obstacles for the delivery of CRISPR system in vivo and shortcomings of CRISPR system itself were also analyzed. Given that intelligent nanoparticles have demonstrated great potential on the delivery of CRISPR system, here we mainly focused on stimuli-responsive nanocarriers. We also summarized various strategies for CIRSPR-Cas9 system delivered by intelligent nanocarriers which would respond to different endogenous and exogenous signal stimulus. Moreover, new genome editors mediated by nanotherapeutic vectors for gene therapy were also discussed. Finally, we discussed future prospects of genome editing for existing nanocarriers in clinical settings.