The cells sense direction is closely related with the proteins that contain PH (pleckstrin homology) domain. PH domain has been found in about 60 proteins, many of which could activate the sequent events of signal transduction via combining with the related binding sites on the surface of chematactic cel ls. The characteristics of this combining are: a.rapid and transient; b.It is only related to the concentration gradient of surroundings outside cells, whi ch is the base of spatial model; c.The distribution of the binding sites on th e cell membrane changes when the researchers altered the position of the chemoat tractant. This is the base of temporal model. To deeply investigate the effects of all kinds of proteins which contain PH domain on cells sense of direction will greatly promote the research of this field, and hence, has great theoretica l significance.

Objective To investigate the effect of histone deacetylases-1(HDAC-1)siRNA on the HDAC-1 protein expression,cell growth and apoptosis in HeLa cells.Methods The HDAC-1 protein was knocked down by HDAC-1 siRNA.HDAC-1 protein was detected by Western blot.The cell growth inhibition was assesses by MTT and clone forming assay.Apoptosis was measured by flow cytometry.Results Forty-eight h after transfection of HDAC-1 siRNA,HDAC-1 protein expression in HeLa cells was down-regulated obviously.The down-regulation of HDAC-1 significantly reduced the colony formation rate,inhibited cell proliferation and induced apoptosis in HeLa cells.Conclusion HDAC-1 siRNA may play an anti-proliferation role in HeLa cells by inducing apoptosis.

Objective To survey and analyze the etiology of pericardial effusion in different age patients. Methods The data of 450 patients who were diagnosed as pericardial effusion were studied retrospectively. The pathogenesis of pericardial effusion were analyzed and compared among the five age groups : juvenile group (0-19 years),young group (20-39 years),middle age group (40-59 years), aged group (60-79 years) and existed advanced age group (≥80 years). Results The different pathogenesis of pericardial effusion existed in different age groups. The first three causes are tumor, tuberculous, heart failure in all patients. As a pathogeny, cardiopulmonary insufficiency induced pericardial effusions constitute the majority (about 50%) in the advanced age group. Tumor, heart failure are the main causes in aged group. In middle age group, tumor and tuberculous are the most frequent pathogenesis, while the tuberculous is the principal causes in juvenile and young groups. Conclusions The rate of tumor, heart failure, lung infection raised along with the age increasing in the patients with pericardial effusion, meanwhile the cases of tuberculoses declined. Attention should be given to this tendency on the diagnosis and treatment of the patients with pericardial effusions.

Objective To investigate the effect of interventional treatment in coronary heart disease (CHD) in elderly patients with brain natriuretic peptide and high-sensitivity C-reactive protein (hsCRP) levels.Methods 130 elderly patients undergoing coronary angiography interventional treatment were selected for the observation group,30 cases with normal coronary arteries angiography were chosen as the control group.In 46 cases of acute myocardial infarction(AMI) group,34 cases of unstable angina pectoris(UAP) group and 50 patients with stable angina pectoris (SAP) group and 30 cases coronary angiography normal control group(NOR),the hs-CRP and plasma brain natriuretic peptide(BNP) differences were determined and compared.Results CHD (AMI group,UAP group and SAP group) in patients with BNP levels were (548.6 ± 200.4) mg/L,(521.0 ± 198.7) mg/L and (175.8 ± 119.4) mg/L,which were significantly higher (59.3 ± 45.7) mg/L in the control group,hsCRP levels only in patients with acute myocardial infarction and unstable angina was significantly increased,the difference was statistically significant (t =13.74,10.65,all P ＜ 0.05).3d after PCI and three months prior CHD hsCRP increased to (45.8 ± 15.3)ng/L,and then decreased to (8.6 ± 4.4) ng/L,significantly different (t =10.76,14.54,all P ＜ 0.05) ; while BNP levels have been falling,first down (247.4 ± 70.1)mg/L,3 months after the falls (69.8 ± 20.0)mg/L,the difference was significant(t =11.47,19.64,all P＜0.05).Conclusion The levels of brain natriuretic peptide,hsCRP are reflections of coronary stenting in patients with coronary heart disease early postoperative inflammatory reaction and evaluate a sensitive indicator of disease,which may play an role in stent restenosis occurs.

Objective To observe the curative effects of Ginkgo dipyridolum injection in the treatment of acute cerebral infarction and its influence on blood rheology and fibrinogen content.Methods 96 patients with acute cerebral infarction were randomly divided into two groups.39 patients in Ginkgo dipyridolum group were treated with 20 ml Ginkgo dipyridolum injection plus 500 ml saline intravenously,once a day.57 patients in control group were treated with Xuesaitong injection 10 ml plus 500 ml saline intravenously,once a day.All treatment lasted for 14 days.The score of neurological deficit was evaluated to assess the clinical efficiency,the changes of indexes of blood rheology,fibrinogen content and skull CT in the two groups were observed before and after treatment.Results Total effective rate(94.9%)in the Ginkgo dipyridolum group was signifcantly higher than that in the control group(78.9%)(P

Stem cells are a group of undifferentiated cells with indefinite self-renewal and pluripotent differentiation ability.They are able to differentiate into precursor/progenitor cells and a variety of cell types and further regenerate new cells to be involved in the repair and rebuilding of injured tissue.Therefore,stem cells are becoming the major research objects in the study on tissue engineering therapy and regenerative medicine.As a sense organ composed of several kinds of neurons and other cells,eyes possesse the dominant superiority in stem cell transplantation therapy because of its good operation controllability and visuality,less demand for seed cells and low rejection after transplantation.These advantages have aroused growing interesting of the fundamental research and clinical trail in stem cell transplantation for irreversible eye diseases.Some exciting advances in the field of stem cell fundamental research,several phase Ⅰ/Ⅱ clinical trials are in progress.The patients with degenerative eye diseases for phase Ⅰ clinical trials are in recruiting in China to evaluate future curative effect and security of stem cell-based therapies.Currently,several issues in stem cell-based eye disease therapies are still pending.We discuss the updated development of stem cell-based transplantation in ophthalmology and future researching direction in order to help ophthalmological researchers to understand the concepts and research strategies.

Objective To observe the sub-clinical inflammation and insulin resistance (IR) in patients with cerebral infarction(CI). Methods The levels of serum C-reactive protein (CRP),interleukin-6 (IL-6),fasting insulin (Fins),fasting plasma glucose (FPG),blood lipids and blood pressure were measured in 114 patients with CI (CI group). The insulin sensitivity index (ISI) was observed and the correlation between ISI and indexes of inflammation were analysed. The results were compared with 70 patients without CI(control group). Results (1) The levels of serum CRP [(2.73?0.87) mg/L]and IL-6[(0.14?0.03) ng/ml] in CI group were significantly higher and ISI(-4.31?0.53) was significantly lower compared with control group[CRP(1.63?0.93) mg/L,IL-6(0.12?0.03) ng/ml,ISI(-3.92?0.43)] ( all P8.2 mg/L was significantly lower than that in the patients with the CRP≤8.2 mg/L (-4.18?0.54)(P

Objective To compare the influence of hypothyroid function in neonatal with hyperbilirubinemia by two kinds blue-light treatment way.Methods 36 cases were divided into the Bilibed infant blue light the bed treatment in 18 cases(group A) and the traditional warm box with the both blue light treatment in 18 cases(group B).The influence of hypothyroid function in neonatal with hyperbilirubinemia were observed after treatment and before treatment.Results The contents of bilirubin in two group after treatment were obviously lower than that before treatment(P0.05).Conclusion The therapeutic of Bilibed method can get the better therapeutic effect than the traditional warm box with the both blue light treatment for the neonatal with hyperbilirubinemia.The change of thyroid function was no influence.

Objective By using optical coherence tomography (OCT) to observe the residual subretina fluid changes in fovea that may explain visual recovery after anatomically successful repair of rhegmatogenous retinal detachments (RD). Design Prospective observational case series. Participants 34 patients(35 eyes) with successful retinal reattachment after macula involving RD repair. Methods 34 patients were followed up at 3 days, 15 days, 2 months and 6 months after sclera buckling. The best corrected visual acuity(BCVA), eye fundus status were recorded. The OCT images through the center of the fovea were obtained. Main Outcome Measures The OCT images and BCVA after RD repair. Results The BCVA and OCT images were obtained 3 days to 6 months postoperatively. There were 3 eyes, 5 eyes, 9 eyes and 23 eyes respectively with residual subfovea fluid absorbed totally at 3 days, 15 days, 2 months and 6 months. In the residual subforvea fluid group, the mean visual acuity increased at 0.124?0.013. In the subfovea fluid absorbed group, the mean visual acuity increased at only 0.019?0.006. Conclusions After anatomically successful repair of RD performend with sclera buckling, the minimal residual subforvea fluid will exist for a long time and will be absorbed slowly. This may explain slow increase of postoperative visual acuity after anatomically successful repair.

Objective:To study the protective effects of exogenous nucleotides on lipopolysaccharide(LPS)immunnostimulation and its mechanism.Method:Forty healthy Kunming mice were randomly divided into five groups:control group,nucleotides(NT)groups(4h,18h),nucleotides free(NF)groups(4h,18h).Control group and NF groups were fed with nucleotide-free diet.NT groups were fed with nucleotide-supplemented diet(0.25% nucleotides).On D 15,mice were lavaged with physiological saline(control)or LPS,and were killed 4 or 18 h later.Serum,liver,small intestine,and peritoneal macrophage were sampled in germfree state.Results:Hepatic Na+K+-ATPase,intestinal superoxide dismutase(SOD),serum total anti-oxidation ability,peritoneal macrophage-produced interleukin 10(IL-10)were increased,and intestinal malonaldehyde(MDA),serum alanine aminotransferase(ALT),intestinal myeloperoxidase(MPO),peritoneal macrophage-produced interleukin 1(IL-1)were decreased with nucleotides supplement.Conclusion:Exogenous nucleotides can help to maintain oxidation-antioxidation and inflammation-antiinflammation balance,and protect mice from injury under LPS immunostimulation.