Objective To determine whether the raised serum uric acid(UA)level is the independent risk factor for coronary artery disease(CAD).Methods A retrospective study was performed including 118 consecutive CAD patients(male 94,female 24)with average age of(65.79?10.03)and 67 normal angiography patients(male 43,female 24)with average age of(60.75?11.98).All the patients were performed coronary angiography from September 2003 to March 2004.In CAD group,36 cases were stable angina pectoris,28 were unstable angina and 54 were acute myocardial infarction.A fasting blood samples were collected for measurement of serum UA,blood cholesterol,and so on.And all patients were carefully inquired for their history including smoking and hypertension.Results Although raised serum UA was observed in CAD patients(372.31?100.28)mmol/L vs(340.08?81.58)mmol/L(P=0.028),logistic analysis suggested serum UA was not an independent risk factor for CAD.And there was no difference of UA among subgroups in CAD patients.Conclusion The raised serum UA may be a marker of atherosclerosis and is not an independent predictor for CAD.

Objective:To study the role of DR5 in TRAIL apoptotic signal transduction. Methods:BALB/C mice were immunized with recombinant DR5 that contains the full-length extracellular domain of the human DR5. Anti-DR5 mAb was generated by hybridoma. The level of DR5 expression on Jurkat cell line was examined by flow cytometry. The rates of TRAIL-induced apoptosis and anti-DR5 mAb blocking on Jurkat cells were tested by flow cytometry with TRAIL apoptosis kit.Results:The percentage of DR5 expression on Jurkat cells was 94 83%. TRAIL and anti-TRAIL mAb could kill Jurkat cells on dose-dependent, and the killing rate was 90% in the concentration of 50～100 ng/ml. The killing role of TRAIL could be blocked on Jurkat cells pretreated with anti-DR5 mAb. The average percentage of blocking was 90 49%.Conclusion:DR5 plays a very key role in TRAIL induced apoptosis in Jurkat cells.

Objective To investigate the value of the three-dimensional high-resolution magnetic resonance imaging curve planar reformation(CPR)combined with virtual endoscopy reconstruction in vascular compression of trigeminal neuralgia.Methods 65 pa-tients with trigeminal neuralgia performed three-dimensional MR doubly excited balanced steady-state free precession sequence (3D-FIESTA-C),and CPR combined with virtual endoscopy reconstruction was used to evaluate the relationship of neurovascular anato-my.And 60 cases with microvascular decompression (MVD)surgery were analyzed.Results 65 cases of 3D-FIESTA-C CPR com-bined with MRVE showed responsibility vascular with a positive rate of 92.3% (60/65).60 cases of all patients with the results of surgery showed responsibility blood vessels and oppression parts with 3D-FIESTA-C CPR combined with MRVE,and it was found that the findings of oppression had a good consistency with MVD with the diagnose accordance rate of 95% (57/60).Conclusion Three-dimensional high-resolution magnetic resonance imaging CPR combined with virtual endoscopy reconstruction can be a new method to clearly and accurately show the anatomical relationship between blood vessels and nerves,which helps to improve the posi-tive rate of detection and to give operative guidance.

Objective To evaluate the use and effectiveness of Human-Computer Interaction (HC1) -based risk prediction of diabetes among Chinese adults.MethodsHCI-based risk prediction of diabetes was performed in 639 non-diabetics aged 23 to 61years old.Risk prediction results,main risk factors of diabetes and helpful suggestions were reported and used for self-management.After l-year follow-up,the participants received another assessment to find the changes of disease risk and risk factors.Non-parametric or Chi-square test was used for comparison of continuous or categorical variables,respectively.Receiver Operating Characteristic (ROC) curve was used to calculate the sensitivity and specificity of HCI.Results After1-year follow-up,the incidence of diabetes per year was1.4％,and all newly diagnosed diabetes was found in high-risk individuals.The proportion of high-risk individuals was 56.8％ and 57.9％before and after follow-up ( x2 =0.36,P ＞ 0.05 ).In comparison with baseline,average risk score of high-risk individuals was significantly declined ( 2.25 vs 2.91,Z =- 4.32,P ＜ 0.05 ).Oversized waist circumstance,higher total cholesterol (TC) and lower high-density lipoprotein cholesterol (HDL-C) was identified in 76.2％,36.2％ and 3.8％ of high risk individuals at1year,lower than those of baseline ( 87.3％,42.2％ and12.4％,respectively ; x2 values were 30.56,6.05 and 22.26,respectively; all P ＜0.05) ; although the prevalence of hypertension was higher (23.5％ vs18.1％,x2 =11.11,P＜0.05).Conclusions HCI and effective control of risk factors could prevent the development of diabetes in high risk individuals.

AIM: To study the effect of cigarette smoke medium(CSM) on the gene expression and activity of gelatinases from alveolar macrophages(AMs) in the rat,and then to explore their role in the pathogenesis of chronic obstructive pulmonary disease(COPD).METHODS: AMs were obtained from BALF of the rats that had smoked for 12 weeks.CSM was produced following the method of Wirtz and colleagues,and the cultured AMs were respectively stimulated for 24 h by 0%,1%,3%,5%,10%, 15% CSM.The mRNA levels of MMP-9 and MMP-2 were detected by semi-quantitative RT-PCR,and the enzyme activity was measured by Zymography.RESULTS: When the concentration of CSM was below 5%,the expression and activity of MMP-9 and MMP-2 signficantly increased with the concentration of CSM in a dose-depended manner(P

Objective To investigate the effect of paclitaxel drug-coated balloon (DCB) dilatation in treating coronary in-stent restenosis (ISR) occurring after drug-eluting stent (DES) implantation,and to observe the long-term changes of the target vascular lumen area in order to clarify the curative effect of paclitaxel DCB in treating ISR.Methods Four patients with ISR whose clinical condition met the DCB indication were selected.According to the standard process,sufficient pre-expansion of ISR was performed first,then paclitaxel DCB dilatation was carried out to dilate the lesion segment of ISR,and no stent was implanted.Both coronary angiography and intravascular ultrasound (IVUS) were performed immediately after the treatment as well as 9 months to measure the minimum lumen area (MLA) and cross-sectional area (SA) of the stent,and the intimal hyperplasia was also been evaluated.Results In all 4 patients,angiography performed immediately after paclitaxel DCB dilatation showed that neither dissection of the dilated segment of the target artery nor obvious residual stenosis was observed.Angiography performed 9 months after the treatment revealed that all dilated segments of the target arteries were patent,and no pronounced restenosis of stent segment was seen.IVUS examination was indicated that MLA became enlarged,SA showed an increasing trend,and intimal hyperplasia showed a tendency to be inhibited.Conclusion For the treatment of ISR,pure paclitaxel DCB dilatation can obtain long-term lumen area enlargement,thus,repeated stent implantation can be avoided,which,in turn,can reduce the risk of ISR recurrence.Paclitaxel DCB dilatation can locally release paclitaxel,which has curative effect on the coronary artery wall to inhibit the excessive proliferation of intima.

Objective: To assess the effects of subchronic intake of high-dose Dendrobium officinale on body weight, food intake, food utilization, and blood biochemical parameters in rats, so as to provide insights into assessment of edible safety of D. officinale. Methods: Eighty SPF-grade SD rats were randomly divided into the low-, medium- and high-dose groups and the control group, of 10 male and 10 female rats in each group. Rats in the low-, medium- and high-dose groups were administered with D. officinale feeds at doses of 2.0, 4.0, and 8.0 g/kg body weight, respectively, while animals in the control group were given basic diet for successive 13 weeks. The rat body weight, food intake, food utilization, and blood biochemical parameters were compared between groups. Results: Normal diet and activity was seen in all rats, and no abnormal syndromes, signs or deaths were found during the study. There were no significant differences in rat body weight, food intake, total weight gain, total food intake, alanine aminotransferase, aspartate aminotransferase, urea nitrogen, creatinine, triacylglycerol, cholesterol, total protein, albumin, albumin/globulin ratio or blood glucose among four groups (P>0.05). The food utilization 7 weeks post-administration [(8.71%±0.78%) vs. (10.54%±1.37%), P<0.05] and the total food utilization [(18.00%±0.41%) vs. (19.51%±1.21%), P<0.05] were significantly lower in male rats in the high-dose group than in the control group. Conclusion Subchronic intake of high-dose D. officinale shows no toxicity in rats, and reduced food utilization may be associated with the health function of D. officinale in male rats.

OBJECTIVETo establish the growth curve of Siraitia grosvenorii and analyze the correlation between seed and growth of fruit.

METHODThe growth curve fitting function was applied for the study of correlation between seed and fruit growth.

RESULTThe significant positive correlation existed between seed and horizontal diameter x vertical diameter, not between seed and flesh weight of single fruit.

CONCLUSIONThe growth curve is a reciprocal function, and seed is one of major factors to influencing the size of fruits and the shape of fruit.

Serum of 79 workers exposed to silica and 25 healthy controls cases were determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). 7 protein peaks were selected and used by artificial neural network (ANN) to establish a diagnostic model. A blinded test showed that accuracy, sensitivity and specificity were 91.35%, 93.69%, and 84.52%, respectively. The diagnostic pattern was also established to distinguish each stage of silica-exposed population. The diagnostic pattern worked excellently with 89.23%, 94.20% and 92.37% of accurate rate for classifying phase 0, phase 0+, and phase I of silicosis, respectively.
Biomarkers ; blood ; Blood Proteins ; analysis ; Humans ; Neural Networks (Computer) ; Sensitivity and Specificity ; Silicosis ; blood ; classification ; diagnosis ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; methods

Objective To investigate the curative effect and safety of percutaneous intradiscal injection of ozone combined with epidural injection of collagenase for the treatment of prominent lumbar disc protrusion.Methods A total of 541 patients with lumbar disc protrusion were included in this study.All the patients fulfilled the following criteria:the fibrous ring was broken,the protruded extent of nucleus pulposus was less than 10 mm and the dura sac and/or nerve root was compressed.The intradiscal injection of ozone combined with epidural injection of collagenase was performed in all patients.The patients were followed up through further consultation,letters,telephone or other ways.Five hundred and forty-one cases were followed up for 2-3 months(short-term),312 cases for 4-12 months(medium-term)and 115 cases for 13-18 months(long-term).The short-term,medium-term and long-term curative effects were evaluated with unified curative criteria,and the results were compared with that obtained with the treatment of epidural injection of collagenase only.Results The short-term,medium-term and long-term effective rate of selected cases was 95.9%(519/541),90.4%(489/541)and 87.2%(472/541),respectively.When combined application of two methods was performed,different degree of shrinkage of the protruded nucleus pulposus was obviously observed,with a mean shrinkage degree of 30.5%.The short-term,medium-term and long-term effective rate of simple collagenase chemonucleolysis was 89.5%,82.4%and 80.4%,respectively.Conclusion Intradiscal injection of ozone combined with epidural injection of collagenase is an effective treatment for lumbar disc protrusion when the fibrous ring is broken,the protruded extent of nucleus pulposus is less than 10 mm and the dura sac and/or nerve root is compressed.Its curative effect is superior to simple collagenase chemonucleolysis.Ozone is of clinical significance for producing the shrinkage of the nucleus pulposus.