AIM: To evaluate colon-oriented delivery characteristics of berberine hydrochloricde(BH) containing carboxymethyl konjac glucomannan(CMKGM) pellets. METHODS: BH-containing CMKGM pellets(pellets group) and BH-containing carboxymethyl cellulose suspension(control group) were intragastric administrated to rats at the dose of 50 mg/kg,respectively.Blood samples were obtained from the rat femoral artery,the gastric、entric、cecal、colonic tissues and their contents sampled at a given interval to measure the concentration of BH by HPLC.The bar charts of relative content of BH in different parts of the gastrointestinal tract and theirs contents were drawn.Drug delivery index(DDI) was calculated. RESULTS: Compared with the control group,the concentration and distribution of BH in gastric、enteric tissue and their contents decreased significantly,but in cecal、colonic tissue and their contents less at first,and more than the control group after 2～6 h.The DDI values of the pellets to gastric,enteric,cecal,colonic tissue and their contents were 0.392 4,0.478 6,3.916,4.193,(0.162 8,)0.619 4,3.843,4.087 against the control group,respectively. CONCLUSION: CMKGM pellets may be a useful colon-specific drug delivery system for BH.

Factors which influence the extraction of Chinese materia Medica were comprehensively studied,by ex-tracting each single herb component in the compounded preparation Sinitang as the sample,and determiningthe extraction rate as well as the content of main active principle in each extract as the evaluation criteria. Thegeneral ru1e for the extraction of single herb material was then investigated. The results indicated that it isbetter to desigm the experiment in accordance with it's characteristic object with selection of proper evalua-tion criteria to obtain an optimum process for the extraction of Chinese traditional drugs.

Recent studies in the realm of metabolic chemistry of traditional Chinese drugs (TCD),appeared in literature were reviewed. Methods for such study were enumerated and commented upon.Some problems that arised in the study were discussed. It was suggested that the study of metabolic chem-istry on TCD and its compounded preparations should be further pursued.

Objective To establish an HPLC method for determination of gastrodigenin concentration in brain tissue of mice and investigate its pharmacokinetics after intragastric administration of gastrodin.Methods The brain homogenate was extracted with acetoacetate and analyzed by HPLC method.The separation was performed on a Diamonsil C18 column(250 mm ? 4.6 mm,5 ?m) under the following chromatographic conditions: mobile phase,acetonitrile-water(10.5∶89.5);column temperature,25 ℃;flow rate,1.0 mL/min;detection wavelength,221 nm;and sampling amount,20 ?L.Results The calibration curve showed good linearity within the concentration range of 50-1 616 ng/mL(r= 0.999 6).The relative recoveries were 93.8%-95.1%,and the RSDs of the intra-and inter-day precision were less than 10%.The concentration-time profile of gastrodigenin in brain tissue of mice showed double peaks(tmax1=15 min,tmax2=90 min).The AUC was 52 822.5 ng?min/g,and t1/2(ke) was 54.8 min.Conclusion The analytical method established for assay of gastrodigenin in brain tissue of mice is sensitive and accurate.The result indicates that gastrodin could rapidly distribute to the brain,be metabolized into gastrodigenin,and be eliminated after oral administration.

Object To study the pharmacokinetics of cinnamic acid in BAOXIN PILL * in rat. Methods Plasma concentration of cinnamic acid was determined by HPLC under the following conditions: column: Hypersil ODS C 18 (150 mm?4.6 mm, 5 ?m); column temperature 30 ℃; mobile phase methanol-1% acetic acid (45∶55); flow rate 0.5 mL/min; detection wavelength 273 nm; aliquot injected 10 ?L. Results A method for the determination of plasma cinnamic acid concentration by HPLC was established. Regression equation of cinnamic acid peak area (Y) and plasma concentration (X) was found to be: Y= 4 973.534 8 +42 867.96 678 X; correlation factor r=0.999 8; rate of cinnamic acid recovery from plasma=97.50% and RSD=1.33%; detection limit=0.15 ng; minimal dectable concentration in rat plasma=75 ng/mL. Absorption pattern of cinnamic acid after ig does showed linearity, corresponding to a first order absorption and elimination of open chamber model. The t 1/2 (K a)=7.12 min; t max =53.29 min, C max =0.20 ?g/mL, and t 1/2 (Ke)=340.74 min. Conclusion The pharmacokinetic parameters obtained in this study may be attributed to the combined action of cinnamic acid and other constituents in the compound preparation of BAOXIN PILL.

Objective To establish a method for the determi nation of emodin and chrysophanic acid in Compound Rhubarb Spray by HPLC.Methods The ODS column was applied.methanol∶water(87∶13)served as mobile phase.The detectio n wavelength was at 254nm and the flow r ate was 0.65ml /min.Results The average recovery of emodin was 99.48%with relative standard deviation being 1.75%(n=9)and the average recovery of chrysoph anic acid was 101.46%with rela-tive standard deviation being 2.85%(n=9).Conclusion The method was simple,rapid and reli able.It is suitable for the quality control of Compound Rhubarb Spray.

AIM: To optimize the best prescription of micro-emulsions of volatile oil in rhubarb of compound liquid spray by triangular phase diagram METHODS : Poloxamer-108 was used as emulsifiers,absolute alcohol as assistant emulsifiers,volatile oil from Lonicera dasystyla and Curcuma longa as the oil phase,the proportion between the emulsifier and append-emulsifier was established RESULTS : Optimized ratio of Poloxamer-108 and alcohol was 1∶10,the volatile oil in prescription formed micro-emulsions,distributing stably in the spray CONCLUSION : The study of micro-emulsions of volatile oil in compound liquid preparation of traditional Chinese medicine by triangular phase diagram offered a short-cut for resolving its stability. The experiment also suggested that the micro-emulsions could increase solubility of insoluble volatile oil from TCM.

AIM:To establish a method of optimizing the formulation of resveratrol solid lipid nanoparticles(SLN)quickly and accurately by uniform design in combination with central composite design and response surface.METHODS:Firstly,uniform design was used to optimize the main factors from the five influence factors such as entrapment efficiency,drug loading,average diameter,and PDI(Polydispersity Index).Then,on the ground of it,the main factors determined by unifrom design were optimized by central composite design with three evaluation parameters and response surfaces were nonlineated according to best-fit mathematic models,so optimized formulation was obtained.RESULTS:According to the optimal conditions,the entrapment efficiency,the drug loading,the average diameter and the PDI(Polydispersity Index)of the prepared Res-SLN were 56.77%,2.59%,167 nm and 0.176,respectively.CONCLUSION:The design of combination of uniform design with central composite design is a feasible and convenient method to optimize the prescription of Res-SLN.

Platelet play an important role in cerebral ischemial nerve injury. Aspirin (ASA) had been used to treat and prevent stroke in clinic. 30 rabbits were randomly divided into A, B and C groups. In group A ASA was given orally at a daily dosage of 15 mg/kg per rabbit for 5 days before cerebral ischemia; group B cerebral ischemia without giving ASA, and group C was normal rabbits as controls. The cerebral ischemial model was produced by occluding bilateral carotid arteries and bleeding from femoral artery. The results indicated that there was an obvious decrease of PAgT and TXA_2 and had no significance changes in free radicals increasing and Ca~(2+) rising from cerebral tissue in group A. The cerebral edema of group A was less severe than group B. It seemed that ASA had a protective effect on the nerve injury of cerebral ischemia. The derangement of ASA, platelet, free radicals and calcium ions interrelation and their significance on the nerve injury should be further studied.

Objective: To establish the determination method of ester alkaloids in Shiwuweirupeng Capsules. Methods:The chromatographic method was carried out on Aichrom TM C 18 column using CH 3OH 0.05 mol?L -1 KH 2PO 4 CH 3COOH-(CH 3) 2CHOH(67∶173∶4∶4) as a mobile phase. The detection wavelength was set at 230nm. The flow rate was 1mL?min -1 . The column temperature was 35?C. Results: The benzoic acid calibration curves were linear at the ranges of 0.0152～0.076?g( r =0.9998). The average recoveries of aconitine was 93.3% with RSD =1.9%.Conclusion: This method is simple, reliable and provides a reference standard for the quality control of Shiwuweirupeng Capsules.