Objective To investigate the influence of bifidobacterium on inflammatory response of brain traumatic injury patients.Methods According to the sequence of patients'admission time,a total of 60 patients with traumatic brain injury were randomly divided into the research group and the control group,30 cases in each group.The research group was fed intestinal nutrient solution combined with bifidobacterium,and 1 × 108cfu/d bacteria every day.The control group was skimpily given enteral nutrient solution.The WBC counts,C-reactive protein (CRP) and interleukin-6 (IL-6),GCS score and infection status,ICU length of stay and 30 days mortality in the 1 st,5th,10th,15th day of enteral nutrition were recorded by health care professionals.Results The leukocyte count,CRP and inflammatory factors IL-6 of the research group were significantly lower than those in the control group in the 15th day of enteral nutrition[(8.02 ± 2.47) × 109/L vs (10.54 ± 3.98) × 109/L,(28.02 ± 9.43) mg/L vs (70.54 ± 13.92) mg/L,(61.02 ± 22.43) ng/L vs (83.52 ± 14.94) ng/L; t =5.474,6.231,9.343,P =0.029,0.049,0.001] ;The GCS score of the two groups gradually increased,and which of the first 10 days had no significant differ ence between the two groups.In the 15th day of enteral nutrition,GCS score of the research group was significantly higher than the control group [(12.02 ± 2.12) point vs (10.12 ± 4.94) point,t =9.744,P =0.000].The incidence rate of infection of research group was significantly lower than that of the control group,but ICU length of stay shortened (16/30 vs 27/30,x2 =9.610,P =0.002).No significant difference was observed in 30-day mortality between the two groups.Conclusion Adding an appropriate amount of probiotics in enteral nutrition on regular basis can significantly reduce the body's inflammatory response and the incidence of infection in patients,shorten hospitalization days.

Objective To evaluate the clinical efficacy of acupuncture-moxibustion in treating deglutition disorders after cerebral stroke.Method By computer and manual literature retrieval, eligible randomized controlled trials (RCTs) were collected for systematic review by using the Cochrane method, and the meta analysis was performed by using Revman 5.2.Result Nine studies were recruited, covering 577 subjects of deglutition disorders due to cerebral stroke. The total effective rate of the treatment group was significantly higher than that of the control group [RR=0.36, 95%CI (0.25,0.50), Z=5.80 (P<0.00001)]. The treatment group was superior to the control group in improving the water drinking test (χ2=108.73,P<0.00001).Conclusion Acupuncture can produce a content therapeutic efficacy in treating deglutition disorders due to cerebral stroke, which still requires high-quality large-sample-size clinical RCTs for further verification.

ObjectiveTo observe the effect of different needle-retaining lengths on the clinical efficacy of acupuncture in treating post-stroke shoulder pain.MethodSixty patients were randomized into a 30 min group and a 60 min group, 30 cases in each group. The same acupuncture treatment protocol was adopted in the two groups, and the acupuncture treatment was given once a day, 5 sessions a week, for 20 times in total. The Visual Analogue Scale (VAS), shoulder pain frequency, and Fugl-Meyer Assessment (FMA) were observed before treatment and after 4-week treatment.ResultThe scores of each index were significantly improved in both groups after treatment (P<0.01), and there were significant differences in comparing each index score between the two groups after treatment (P<0.05,P<0.01).ConclusionIn the treatment of post-stroke shoulder pain with acupuncture, 30 min needle-retaining is better than 60 min in comparing the analgesic effect.

0 05);②Coixan(100 mg?kg -1 ) could prevent rats from diabetes induced by alloxan. the blood glucose and the area of curve in the preventive group was lower than in the alloxan group. The activity of superoxide dismutase(SOD) in the preven tive group was higher than in the alloxan group. The morphological observation of ? cells confirmed that coixan could markedly prevent ? cells from injury induced by alloxan and there was no obvious change in the appearance of islet ? cells in the preventive group. CONCLUSION Coixan markedly prevents ? cells from injury induced by alloxan.

To investigate the effect of the disequilibrium of calcium homeostasis on the apoptosis of MDCC-MSB1 cells induced by lanthanum chloride(LaCl3 ), MDCC-MSB1 cells was conventionally cultured in RPMI1640, which was tured for 24 h, MTT was utilized to detect the cell multiplication inhibition ratio,DNA ladder and TUNEL to detect apoptosis,the Fura-2/AM as the probe to detect the [Ca2+]i within cells. The results indicated when LaCl3 concen and [Ca2+]i also increased,and presented a dose-effectiveness relationship. LaCl3 could restrain the proliferation of MDCC-MSB1 cells,suggesting their apoptosis induced by changing [Ca2+]i.

Objective To explore the current occurrence of upper gastrointestinal bleeding (UGIB)in elderly patients and its possible risk factors and treatment program. Methods The clinical data of 758 elderly coronary heart disease patients with aspirin and clopidogrel co-therapy were collected, including past medical history,common condition,clinical medication,biochemical indicator and clinical turnover. The follow-up ended on the dates of a first occurrence of UGIB and death or after stopping co-therapy above 10 days. The follow-up time was 6 months. Results UGIB occurred in 48 patients,the occurrence rate was 6.3% (48/758). The occurrence rate of UGIB in proton pump inhibitor (PPI), H2-receptor antagonist (H2RA), gastric mucosa protective agents plus PPI, gastric mucosa protective agents plus H2RA and control was 3.7% (4/108),5.0% (12/240),1.4% (1/70),2.9% (3/102),11.8% (28/238). There were statistic differences in different methods (x2 = 18.63,P = 0.001). Conclusions The occurrence rate of UGIB combined with aspirin and clopidogrel co-therapy in elderly is high. Co-prescription with PPI and gastric mucosa protective agents is associated with a lower risk.

Objective To explore morphine usage in emergency department (ED) and its effect on outcome in elderly patients with acute decompensated heart failure (ADHF) in a single Chinese center. Methods A prospective observational analysis was performed in elderly patients aged 65-99years with newly diagnosed ADHF, who were treated and admitted from ED of our hospital. The data including patients' demographic characters, past medical history, initial presentations and medication taking were collected and analyzed. According to using morphine or not, the patients were divided into two groups. The effects of morphine on mechanical ventilation, hospitalization stay and in-hospital mortality were also evaluated. Results The 82 patients were enrolled in this study, 52 males and 30females. In the 41 (50. 0%) patients using morphine (morphine usage group), the foregone incidence rates of coronary heart disease, type 2 diabetes mellitus and stroke were less, the levels of troponin T and random blood sugar were increased in morphine usage group than in non-morphine usage group.No other differences were found in initial presentations between the two groups. The patients often theophylline before morphine administration. And they had a higher prevalence of mechanical ventilation (95%CI: 1.22-7.41), but no differences were found in hospitalization stay and in-hospital mortality between the two groups. Conclusions Morphine is still as a common medicine in treatment of ADHF in ED. Morphine usage can increase prevalence of mechanical ventilation without influencing hospitalization stay and in-hospital mortality even in elderly ADHF patients. Morphine may be a safe medication in the rational treatment of early-stage ADHF in elderly patients with mechanical ventilation support.

AIM: To establish an HPLC method for determining sophocarpine,sophoridine and oxymatrine in Sophora flavescens Ait,so as to investigate their contents in different years and different parts. METHODS: An Elite Hypersil NH2 column(250 mm ? 4. 6 mm,5 ?m) was used with the mobile phase being acetonitrile-absolute alcohol-3% phosphoric acid solution(82 ∶ 10 ∶ 8),flow rate being 1 mL/min,determinating wavelength being 220 nm,the column temperature being 26 ℃,The injection volume was 5 ?L. RESULTS: The calibration curves of sophocarpine,sophoridine and oxymatrine were in good linearity over the ranges of 0. 004 99 - 0. 149 7 ?g(r = 0. 999 9),0. 025 08 - 0. 752 25 ?g(r = 0. 999 9),0. 075 38 - 2. 261 25 ?g(r = 0. 999 9); and the average recovery of sophocarpine,sophoridine and oxymatrine was 99. 91% ,99. 26% ,100. 27% with RSD of 1. 11% , 0. 82% ,2. 18% respectively. CONCLUSION: The HPLC method shows a good separation,reproducibility and accuracy,there are obvious differences in the contents of three alkaloids in different years and different parts of Sophora flavescens Ait. The results provide important data for quality evaluation and utilization of Sophora flavescens materials.

Objective To study the mechanism of changes in blood gas and hemodynamics in aged rats with pneumonia. Methods Forty aged male Sprague-Dawley(SD) rats were randomly divided into three experimental groups and the control group. They were contrasted by 40 non-aged male SD rats. Hemodynamic parameters and blood gas were measured, and morphological changes of heart and lung were observed. Plasma levels of tumor necrosis factor-? (TNF-?) was examined by immunohistochemical method. Results PaO 2 and SaO 2 were lower in rats with pneumonia than those in the control rats(P

OBJECTIVE: To compare the effects of different factor combinations on diabetic nephropathy(DN)rats model and to determine the preferred plan for the establishment of DN rats model.METHODS:A total of 30 SD rats were randomly divided into 5 group(6 rats in each group):group A was assigned to receive placebo(normal control),group B to receive high-lipids and high-sucrose feeding,group C to receive high-lipids and high-sucrose feeding plus streptozotocin(STZ),group D to receive high-lipids and high-sucrose feeding plus doxorubicin hydrochloride and STZ,group E to receive high-lipids and high-sucrose feeding plus mononephroctomy and STZ 12 weeks later the model rats were put to death for the determination of serum parameters including blood lipid,glycosylated hemoglobin,renal function,aldose reductose(AR),glomerular filtration rate(GFR),SOD and MDA .RESULTS:The rats in group E showed significant increase in blood lipid,in particular the increase of AR,and which also showed significant differences in GFR and oxygen free radical indices as compared with normal group and the other model groups.CONCLUSIONS:The rats model that fed with high-lipids and high-sucrose feeding for 4wk prior to mononephroctomy followed by the postoperative intraperitoneal injection of low dose of STZ 2 weeks later proves to be the optimal one.