1.Pharmacokinetic effect of aspirin on caffeic acid in dengzhanxixin injection
Guoliang DAI ; Shijia LIU ; Changyin LI ; Lei WU ; Shitang MA ; Wenzheng JU ; Hengshan TAN
Chinese Pharmacological Bulletin 2014;(4):570-573,574
Aim To investigate the pharmacokinetic effect of aspirin on caffeic acid in dengzhanxixin injec-tion( DI) . Methods Concentration of caffeic acid in rat plasma was detected by LC-MS/MS after rats were given intravenous administration of DI or DI combined with aspirin by gavage. Pharmacokinetic parameters were calculated by DAS 1. 0 pharmacokinetic software. Results In vivo pharmacokinetic models of caffeic acid were two-compartment open models in both the caffeic acid group and the caffeic acid combined with aspirin group. After compatibility, caffeic acid showed a significant increase in T 12β, with a slight decrease in CL. Conclusions Aspirin can reduce metabolic process of caffeic acid in vivo.
2.HIV-1 drug resistance transmission threshold survey in Dehong prefecture of Yunnan province,2015
Min CHEN ; Jibao WANG ; Hui XING ; Yanling MA ; Jin YANG ; Huichao CHEN ; Shitang YAO ; Hongbing LUO ; Xing DUAN ; Yikui WANG ; Song DUAN ; Manhong JIA
Chinese Journal of Epidemiology 2017;38(7):959-962
Objective To study the HIV-1 drug resistance transmission level in HIV infected persons receiving no antiviral therapy in Dehong prefecture of Yunnan province in 2015.Methods A total of 72 plasma samples were collected from recently reported HIV-infected persons aged 16-25 years in Dehong from January to July 2015 for drug resistance gene detection.Results Forty eight samples were successfully sequenced and analyzed.Among them,31.2% (15/48) were from Chinese,and 68.8% (33/48) were from Burmese.Based on pol sequences,HIV genotypes included URF (52.08%,25/48),CRF01_AE (16.67%,8/48),RF07_BC (10.42%,5/48),subtype B (6.25%,3/48),subtype C (6.25%,3/48),CRF57_BC (6.25%,3/48) and CRF08_BC (2.08%,1/48).One drug resistant mutation site to non-nucleoside analog reverse transcriptase inhibitor (NNRTI) and two drug resistant mutation site to nucleoside analog reverse transcriptase inhibitor (NRTI) were detected in four sequences.Based on the statistical method of HIV drug resistance threshold survey,the prevalence of HIV-1 drug resistant strain was 5%-15%.Conclusions The proportion of Burmese among newly reported HIV-infected individuals aged 16-25 years in Dehong in 2015 was higher.HIV-1 genetic diversity was found in Dehong.The prevalence of HIV-1 drug resistant strain had reached a moderate level in Dehong.
3.A systematic pharmacological investigation of pharmacologically active ingredients in Toujie Quwen granules for treatment of COVID-19.
Shitang MA ; Xue ZHANG ; Jinfeng CEN ; Ge HONG ; Shengwei HONG ; Wenzheng JU
Journal of Southern Medical University 2020;40(8):1072-1080
OBJECTIVE:
To explore the pharmacologically active ingredients in granules (TJQW) for treatment of coronavirus disease 2019 (COVID-19) in light of systemic pharmacology.
METHODS:
We performed database search, literature mining and drug-like index screening to identify the bioactive components in TJQW, the positive drugs for disease treatment and their therapeutic targets. The core disease target was investigated based on the cross-linking interaction of the bioactive components, positive drug and potential disease target, and the target proteins at the key nodes were analyzed by GO and KEGG analyses. Based on the therapeutic targets for COVID-19, virtual screening was conducted to screen the compounds in TJQW and construct the network cross-linking the key bioactive molecules in TJQW, key node targets of the disease, and the related biological pathways.
RESULTS:
We identified 159 compounds in TJQW and obtained 18 core proteins based on the cross-linking of the bioactive components, positive drugs and disease targets. The key node targets consisted of 22 targets including the latest 4 COVID-19 proteins. Virtual screening results showed that at least 14 compounds could bind with the core disease target proteins. The material basis of TJQW for COVID-19 treatment was explained in multi-pathway, multi-component and multi-target perspectives. In terms of the structural characteristics of the compounds, we screened the top 30 molecules with strong binding with the target proteins, among which flavonoids were the predominant components.
CONCLUSIONS
This investigation reveals the therapeutic mechanism of TJQW for COVID-19 involving multiple components, targets and pathways from the perspective of key bioactive molecules, disease key node targets and related biological pathways. We screened 30 active precursors from TJQW, which provides reference for the clinical application and further development of TJQW.
Betacoronavirus
;
Coronavirus Infections
;
drug therapy
;
Drugs, Chinese Herbal
;
Humans
;
Medicine, Chinese Traditional
;
Pandemics
;
Pneumonia, Viral
;
drug therapy
4.Analysis on HIV-1 genetics and threshold of drug resistance in Dehong prefecture of Yunnan province in 2013.
Yanling MA ; Jibao WANG ; Hui XING ; Min CHEN ; Shitang YAO ; Huichao CHEN ; Jin YANG ; Yanling LI ; Song DUAN ; Email: DHDUANSONG@SINA.COM. ; Manhong JIA ; Email: JIAMANHONG@HOTMAIL.COM.
Chinese Journal of Epidemiology 2015;36(6):584-588
OBJECTIVETo study the HIV-1 genotypes and transmitted drug resistance (TDR) in Dehong prefecture of Yunnan province in 2013.
METHODSReferring to the guidelines for HIV drug resistance threshold survey (HIVDR-TS), 54 plasma samples of recently reported HIV-infected individuals, aged between 16 and 25 years, were collected in Dehong prefecture from January to August 2013. Genotyping of partial pol gene was performed by using reverse transcriptional PCR. HIV-1 genotype. Prevalent levels of HIV-1 drug resistance transmission were analyzed.
RESULTSForty-eight plasma samples were successfully sequenced and analyzed. Among them, 45.8% were Chinese and the rest 54.2% were all Burmese. Based on pol sequences, identified HIV genotypes included subtype C (41.7%), URF (31.3%), CRF01_AE (12.5%), CRF07_BC (10.4%), CRF08_BC (2.1%) and subtype B (2.1%), C subtype appeared dominated in Chinese while URF was dominated in Burmese. One drug resistant mutation to non-nucleoside reverse transcriptase inhibitors (NNRTIs) was detected in one sequence from Burmese. Based on the statistical method of HIVDR-TS, the prevalence of transmitted HIV-1 drug resistance was adjusted as < 5%.
CONCLUSIONDiverse HIV-1 genotypes were found in this study, and the current HIV-1 drug resistant strains transmission was catalogued as at low prevalence level, in Dehong. To prevent the increase of the prevalence of transmitted HIV-1 drug resistance, standard treatment and scientific management for people living with HIV/AIDS should be strictly followed. Meanwhile, relevant surveillance, including drug resistance surveillance should also be performed among cross-border migrant population.
Anti-HIV Agents ; pharmacology ; therapeutic use ; China ; Drug Resistance, Viral ; genetics ; Genes, pol ; Genotype ; HIV Infections ; drug therapy ; virology ; HIV-1 ; drug effects ; genetics ; Humans ; Mutation ; Polymerase Chain Reaction ; Prevalence ; Reverse Transcriptase Inhibitors
5.Study on HIV-1 related genetics and threshold on drug resistance in Dehong prefecture of Yunnan province in 2012.
Min CHEN ; Jibao WANG ; Hui XING ; Yanling MA ; Shitang YAO ; Huichao CHEN ; Jin YANG ; Yanling LI ; Song DUAN ; Manhong JIA
Chinese Journal of Epidemiology 2014;35(8):909-912
OBJECTIVETo study the HIV-1 genotypes and transmitted drug resistance (TDR) in Dehong prefecture of Yunnan province in 2012.
METHODSReferring to the guidelines for HIV drug resistance threshold survey (HIVDR-TS), 60 plasma samples of recently reported HIV-infected individuals between 16 and 25 years old were collected in Dehong prefecture from January to August 2012. Genotyping of partial pol gene was performed by using reverse transcriptional PCR. HIV-1 genotype and the prevalent levels of HIV-1 drug resistance transmission were analyzed.
RESULTS52 plasma samples were successfully sequenced and analyzed. Among them, 59.6% were Chinese, and the rest (40.4%) were Burmese. Based on pol sequences, identified HIV genotypes would include unique recombinant forms (URFs, 38.5%), subtype C (34.6%), CRF01_AE (21.2%), CRF08_BC (3.8%), and subtype B (1.9%). One drug resistant mutation to non-nucleoside reverse transcriptase inhibitors (NNRTIs) was detected in respective two sequences. Based on the statistical method of HIVDR-TS, the prevalence of transmitted HIV-1 drug resistance was adjusted as a moderate level (5%-15%).
CONCLUSIONDiverse HIV-1 genotypes were found in this study, and the current HIV-1 drug resistant strains transmission was catalogued as moderate prevalence level in Dehong.
Adolescent ; Adult ; China ; epidemiology ; Drug Resistance, Viral ; genetics ; Female ; Genotype ; HIV Infections ; epidemiology ; virology ; HIV-1 ; drug effects ; genetics ; Humans ; Male ; Young Adult
6. Study on HIV-1 genetics and drug resistance among HIV infected individuals who initiated antiretroviral therapy in Dehong Dai and Jingpo autonomous prefecture, 2017
Huichao CHEN ; Jibao WANG ; Hui XING ; Yanling MA ; Xing DUAN ; Jie DAI ; Xiaomei JIN ; Shitang YAO ; Song DUAN ; Min CHEN
Chinese Journal of Epidemiology 2019;40(8):982-987
Objective:
To understand the distribution of HIV-1 genotypes and the status of drug resistance among people living with HIV who had prepared to initiate antiretroviral therapy (ART) in Dehong Dai and Jingpo autonomous prefecture (Dehong).
Methods:
A total of 170 adults with HIV were recruited in Dehong from January to June 2017, before initiating ART. HIV-1