[Objective]To discuss the influence of posterior cervical titanium internal fixation system on magnetic resonance image(MRI).[Method]Six cases that had operated in Changhai hospital with posterior cervical titanium internal fixation system performed MRI scan.The MRI system were 1.0-T or 1.5-T scanner.Patients all performed sagital T1,T2 and axial T1-weighted image scan under turbo spin echo(TSE)sequence,then the artifacts and its influence on the MRI image were analyzed.[Result]Mean artifact size ranged from 2 to 3 times of actual screw size.The artifact was similar to the primary shape of internal fixation system with low intensity signal on center and high intensity signal on the margin.The details in the spinal canal can be distinguished.The rod had small artifact,but the laminar hook had large artifact which obscured the posterior structure of spinal canal,and the artifact of pedicle screw had influence on lateral spinal canal,nerve root and vertebral artery.[Conclusion]Patients with posterior cervical titanium internal fixation system should choose Turbo spin echo(TSE)sequence for MRI scan,if the spin echo is performed,the TE should be minimized.

Objective: To observe the effect of treatment for proliferative knee osteoarthritis (KOA) using combined acupuncture and medicated compression. Method: All 328 cases were randomized into an observation and control group, 164 cases in each. Combined acupuncture and medicated compression were adopted in the observation group, while oral administration of Zhui Feng Tou Gu Wan (Wind-removing and Bone-penetrating Pills) were adopted in the control group. The clinical effects in the two groups were evaluated 40 days after the treatment. Result: There were significant differences in both marked and total effective rates (P<0.05). Conclusion: Combined acupuncture and medicated compression can obtain better effect for KOA than oral administration of the Wind-Removing and Bone-Penetrating pills.

Objective To investigate the reliability and validity of the Chinese version of the schizophrenia cognition rating scale ( SCoRS) . Methods 112 cases of schizophrenia or schizophreniform disorder inpatients were recruited and assessed using the SCoRS( Chinese version) ,the brief assessment of cognition in schizophrenia (BACS) and personal and social performance scale(PSP). The principle component analysis,reliability analysis and correlation analysis were used to analyze the data. Results Standardized Cronbach α = 0.964. All the 20 i-tems were significantly correlated. Except item 1 ,9,18,19 ( r=0. 830,0. 890,0. 871,0. 890) ,the interrater correlation coefficiency (ICC) of the rest of the items was over 0.9. The ICC of global rating scores between two raters was 0. 921. Factor analysis procedure identified three factors. Factor 1 reflected the neurocognitive state; factor 2 reflected the social cognitive state;and factor 3 mainly reflected the daily life function. The SCoRS rating scores were significantly correlated with the BACS and PSP scores. Conclusion The validity and reliability of SCoRS are acceptable. SCoRS is a useful tool for assessing the deficits of the cognitive function for Chinese schizophrenia patients.

Objective To investigate the status of the non-psychiatrist' s diagnostic and therapeutic ability of depression/anxiety disorders in 57 General Hospitals.Methods 1 152 non psychiatric clinicians in 57 general hospitals were surveyed.A custom-made questionnaire included the training of mental health-related knowledge which the general hospital physicians received and typical anxiety/depression case analysis.Results Among 1 521 non-psychiatric clinicians,596 (51.74％) clinicians participated the training of psychiatry,562 (48.78％) participated the training of medical psychology and 230(20.97％) clinicians participated the training of Healthy Psychology.In professional setting,59 (5.12％) clinicians participated the training of psychotherapy,255 (22.14％) clinicians had attended related academic symposiums.80(6.94％) clinicians believed that they understand the clinical display of anxiety/depression disorders,52 (4.51％) clinicians expressed the understanding of diagnostic criteria of anxiety/depression disorders and its treatments,while 44(3.82％) clinicians only possessed the knowledge of anxiety/depression disorder treatment.In the typical case analysis,it revealed that 794 (68.89％) clinicians made accurate diagnosis,458(57.68％)clinicians made a choice of medical treatment,764(96.22％) clinicians chose psychotherapy,29 (3.65％) clinicians applied physical therapy,while 438 (55.16％) clinicians combined drug therapy with one or more other therapeutic methods.Binary logistic regression analysis showed that the age(P=-0.093,Exp(B)=0.911)and work experience(P=-0.002,Exp(B) =1.080)significantly contribute to the diagnostic accuracy of non-psychiatrists.Conclusion The non-therapeutic psychiatric clinicians in general hospitals have certain basic knowledge of depression/anxiety disorders with lower level of diagnosis and treatment diagnosis and treatment.And there is bigger difference among different hospitals.

Objective Choroidal neovascularization(CNV) is the main cause of blindness in over 50-years-old population. To establish an available CNV animal model is helpful for us to understand the pathogenesis and management of CNV. Purpose of present study was to observe the role of coagulation with different power of diode laser in laser-induced choroidal neovascularization model of Brown Norway (BN) rats. Methods Coagulation of 810 nm diode laser(8 - 10 spots for each eye) was performed in 36 male BN rats with the spot diameter 75 μm, shutter time 0. 1 s, power 120 mW, 140 mW and 160 mW, respectively, while 6 normal BN rats were used as contrast. CNV was evaluated by fundus examination, fundus fluorescein angiography (FFA), indocyanine green angiography(ICGA) and light microscope on day 1, 7, 14, 21, 28 and 56 after photocoagulation. Results CNV formed on the 7th day after photocoagulation in 120 mW, 140 mW and 160 mW groups and reached the peak on the 21st day according to FFA and ICGA manifestation. Incidence of CNV in 120 mW, 140 mW and 160 mW group was 51. 3%, 91. 8%, 88. 3% on FFA findings and 51.3%, 92.7%, 93.7% on ICGA findings, respectively. In 7 days after photocoagulation, inflammatory cells increased and CNV formed at the lesion. Photocoagulation plaque became thicker with pigment cells proliferating and migrating on 14 days. After that time, inflammatory cells decreased and more collagen fibers emerged. The CNV reminded till the 56th day after photocoagulation. Conclusion CNV model of BN rats can be successfully created using the different power of diode laser (from 120 through 160 mW). CNV rate under the laser coagulation with 140 mW is higher, indicating that the power of 140 mW may be a suitable parameter for diode laser-induced choroidal neovascularization model of BN rats.

Objective:To evaluate the validity and reliability of adapted simplified Chinese Version of the Scoliosis Research Society-22(SRS-22)questionnaire.Methods:The English version of the SRS-22 was translated into simplified Chinese and was subjected to cross-culture adaptation;the final version of the simplified Chinese SRS-22 questionnaire was a consensus reached by an expert committee.Then the simplified Chinese version SRS-22 questionnaire and 36-Item Short Form Health Survey(MOS SF-36)questionnaire were used to survey 87 patients with adolescent idiopathic scoliosis who had been surgically treated;63 patients(72.4%)responded to the SRS-22 questionnaire.The average age of these patients(6 male,57 female)was(17.7?3.1)years(range,14.3-23.8 years).Fifty-six of the 63 patients returned the MOS SF-36 questionnaire.The 2 indicators of reliability were Cronbach's ? of internal consistency and intraclass correlation coefficient(ICC).Concurrent validity of the SRS-22 questionnaire was measured by comparing with domains of the SF-36 questionnaire,which was reflected by Pearson correlation coefficient(r).Results:The Cronbach ? values for 4 domains(function/activity,pain,self-image/appearance,and mental health)were all higher than 0.7;and the Cronbach ? value for satisfaction of management domain was acceptable.The intraclass correlation coefficients for function/activity,pain,self-image/appearance,mental health,and satisfaction domain were 0.74,0.78,0.86,0.81 and 0.84,respectively,demonstrating a satisfactory reproducibility.For the concurrent validity,3 domains had excellent correlation,10 had good correlation,and 21 had moderate correlation.Conclusion:The adapted simplified Chinese Version of the SRS-22 questionnaire has satisfactory reliability and concurrent validity,and might be suitable for post-operation clinical evaluation of Chinese adolescent patients with scoliosis.

Objective:This study was to observe the therapeutic effect of electro-acupuncture combined with Chinese herbal fumigation and application in the treatment of relapsed lumbar disc herniation after lumbar disc surgery.Methods:Eighty-four subjects were randomized into a treatment group and a control group.The treatment group(44subjects)received electroacupuncture plus herbal fumigation and application therapy.The control group(40 subjects)only received electroacupuncture therapy.The therapeutic results were observed and compared afterwards.Results:The total effective rate was 90.9% in the treatment group and 82.5% in the control group.and the difierence was statistically significant(P<0.05).Conclusion:The combined therapy of electroacupuncture with Chinese herbal fumigation and application has better therapeutic effect than mono-electroacupuncture in the treatment of post-surgery relapsed lumbar disc herniation.

Objective To evaluate the reason of the complications after treating by the intermaxillary traction with the titanium screw and put forward the measures of prevention these complications in mandibular fractures.MethodsOne hundred and twenty-six cases with mandibular underwent intermaxillary traction and fixation with tapping titanium screw were observed and counted for their complications in 1-30 days after finishing the operation.Summarized and analyzed the reasons of the complications.ResultsThe rate of complications was 9.52％(12/126),4 cases of titanium screw loosen,2 cases of bad occlusion,1 case of titanium screw fallen off,1 case of titanium screw around infected,1 case of root tip damage,1 case of titanium screw maxillary penetrated into the sinus,1 case of nerve injury,1 case of dental ulcer.Conclusion The intermxillary traction with the titanium screw is simple,safety and healthy method,but the postoperative complications also don't allow to ignore,surgeons should be controlled strictly,standardized operation,try to avoid complications.

Objective To construct recombinant plasmid containing CFP-10 gene of Mycobacterium tuberculosis(MTB).Methods The gene fragment of CFP-10 was amplified by PCR from Mycobacterium tuberculosis H37Rv genomic DNA and cloned to pET-32a(+) vector.The recombinant plasmid pET-32a-CFP-10 was transformed into E.coli BL21 (DE3) and induced by IPTG.Results CFP-10 gene fragment was amplified from genomic DNA of Mycobacterium tuberculosis H37Rv strain,and thepET-32a(+) prokaryotic recombinant plasmid was constructed successfully.The recombinant protein was expressed with the induction of IPTG.Conclusions The prokaryotic expression vector for CFP-10 was successfully constructed and the recombinant protein was highly expressed in E.coli BL21 (DE3),which lays a foundation for its subsequent immunological function study.

The aim of the study was to investigate the sesquiterpene constituents from the rhizomes of Curcuma wenyujin Y．H．Chen et C．Ling．The isolation and purification of the constituents from the 50％ EtOH extracts of the rhizomes were performed with repeated column chromatography over sillica gel and macroporous resin．Eight sesquiterpenes were obtained and identified as wenyujinlactone A(1),neolitamone A(2),zedoarondiol(3),isozedoarondiol(4),aerugidiol(5),curcumol (6),curdione(7) and (1R,10R)-epoxy-(-)-1,10-dihydrocurdine (8) by means of spectral analysis．Among them,compound 1 was found to be a new eudesmane sesquiterpene lactone,whilst compounds 2-5 were obtained from this plant for the first time．