Objective To improve the external irradiation technique for Head-neck neoplasms.Methods 95 patients with Head-neck neoplasms confirmed by pathology,were fixed with head pillow mask , isocenter irradiation,technique utilizing cerrobend block.Radical therapy dose was 72 (70～80)Gy/35～40 fractions in 7～8 weeks for general carcinoma and 50～55Gy/28～31 fractions in 5 6～6 2 weeks for NHL .Preventive dose was 56 (50～60)Gy/25～30 fractions in 5～6 weeks for general carcinoma and 45～50Gy/25～28 fractions in 5～5 6 weeks for NHL.A short and a long-term clinical outcome, acute and late radiation reactions were observed.Results After 1～2 months following radiation therapy,CT scans revealed that the primary lesion complete remission rate was 58 57% , and obvious remission rate was 32 86%.The normal mucous membrane acute radiation reaction rates were G0 56 84% , GⅠ 42 10% , GⅡ 1 05%,respectively.After 1～2 years following radiation therapy,the spinal cord and brain stem radiation injury rate was zoro.Conclusions The improving external irradiation technique may elevate local control rate better,and reduce radiation reactions of patients with head-neck neoplasms.

Objective To analyze the clinical features of severe hand-foot-mouth disease (HFMD)combined with encephalitis.Methods The cases with HFMD in our hospital from September 2009 to September 2010 were divided into two groups including the ordinary case group(n =235) and the encephalitis group (n =88).Clinical manifestations were analysed between the two groups.Results There were significant differences in number of oral ulcers,degree and duration of fever between two groups (P ＜ 0.05).Eighty percent cases of the ordinary group had multiple or dense herpes or ulcers,which was more than that of the encephalitis group.But there were no significant differences in WBC and its classification in peripheral blood between two groups (P ＞ 0.05).There were obvious differences in CK,CK-MB,AST,GLU of blood between two groups (P ＜ 0.05).The contents of protein,glucose and chloride in cerebrospinal fluid have no differences between two groups (P ＞ 0.05),besides the cell count.The cell count in cerebrospinal fluid of cases with enterovirus infected encephalitis was significantly higher than those with CoxA16 infection(P ＜ 0.05).Conclusion The patients with few rash,less oral herpes,ulcers or high levels of CK,CK-MB,GLU should be closely observed to vigilant merger encephalitis.The cases with severe H-FMD have middle or high fever and duration of fever are more than 4 days.The increased cell count in cerebrospinal fluid may imply encephalitis with enterovirus 71 infection.

Objective To investigate the pharmacodynamic action of Yunkang Capsule(YKC).Methods The animals were divided into YKC groups(at high-,moderate-and low-dosage respectively),diphenidol control group,model control group and blank control group.The action of YKC on vertigo and thrombosis,hemorrheologic indexes,piamatral microcirculation and free radicals of the animal models were observed.Results YKC could significantly prolong the latency of vertigo,reduce wet weight of thrombus,decrease blood viscosity,plasma viscosity and hematocrit,promote the dilation of piamatral micro-vessels,increase the amount of interweave microdot,increase plasma SOD activity and decline plasma MDA content of animal models.Conclusion YKC has actions of counteracting vertigo and thrombosis,improving hemorrheology and microcirculation,and clearing away free radicals.

Abnormal expression of annexin A2 contributes to metastasis and infiltration of cancer cells.To elucidate the cause of abnormal expression of annexin A2,Western blotting,immunoproteomics and immunohistochemical staining were performed to analyze differentially ubiquitinated proteins between fresh breast cancer tissue and its adjacent normal breast tissue from five female volunteers.We detected an ubiquitinated protein that was up-regulated in the cancer tissue,which was further identified as annexin A2 by mass spectrometry.These results suggest that abnormal ubiquitination and/or degradation of annexin A2 may lead to presence of annexin A2 at high level,which may further promote metastasis and infiltration of the breast cancer cells.

Objective To study the effect of annexin A5 on the apoptosis of laryngeal cancer cells.Methods Special siRNAs were used to knock annexinA5 down in Hep-2 cell,and RT-PCR and Western blot were applied to identify the efficacy of RNA interference.The flow cytometry assay was performed to detect the Hep-2 cell apoptosis.Results RT-PCR analysis showed that the relative mRNA expression of annexin A5 in siRNA group,negative control group,Lipofectamine2000 group and blank control group were 0.70 ±0.03,1.18 ±0.05,1.17 ±0.06 and 1.23 ±0.07.The relative mRNA expression of annexin A5 in siRNA group was significantly decreased than contrast groups(t =-14.77,t =-13.23,t =-12.99,P ＜0.05).In Western blot assay,the trend of protein expression level was consistent with the mRNA expression levels of annexin A5.The relative levels of proteins in siRNA group,negative control group,Lipofectamine2000 group and blank control group were shown 1.21 ± 0.03,3.88 ± 0.06,3.87 ±0.02 and 3.95 ± 0.08.The relative protein expression of annexin A5 in siRNA group was significantly decreased than contrast groups(t =-70.34,t =-150.62,t =-56.32,P ＜0.05).At the same time in flow cytometry the apoptotic rate of siRNA group,negative control group,Lipofectamine2000 group and blank control group were 4.43％ ±0.12％,13.67％ ±0.22％,13.66％ ±0.12％ and 13.35％ ±0.13％,the difference between the siRNA group and contrast groups was statistically significant (t =-62.50,t =-14.16,t =-11.47,P ＜ 0.05).So after RNA interference,expression of annexin A5 decreased,and the results in the apoptosis inhibition of Hep-2 cell.Conclusion Annexin A5 promotes apoptosis of Hep-2 cells,and it may be a potential therapeutic target for the laryngeal cancer.

Japanese encephalitis virus (JEV) is the leading cause of viral encephalitis in Asia and domestic pigs serve as the amplifying hosts. In the present study, the full genomic sequences of two JEV strains (HEN0701 and SH0601) isolated from pigs in China were determined and compared with other 12 JEV strains deposited in GenBank. These two strains had an 88.8% nucleotide sequence similarity and 97.9% deduced amino acid sequence homology. HEN0701 had high nucleotide sequence and high amino acid sequence identity with genotype I (GI) strains, while SH0601 had high nucleotide sequence and high amino acid sequence identity with GIII strains at both the gene and full genome levels. Further phylogenetic analysis showed that HEN0701 belonged to the JEV GI group and SH0601 was classified as a GIII strain. Analysis of codon usage showed there were a few differences between the GI and GIII strains in nucleotide composition and codon usage for the open reading frames.
Animals ; Cell Line ; Cricetinae ; Encephalitis Virus, Japanese/classification/*genetics ; Encephalitis, Japanese/epidemiology/*veterinary/virology ; Gene Expression Regulation, Viral/physiology ; Genome, Viral ; Molecular Epidemiology ; Phylogeny ; Swine ; Swine Diseases/epidemiology/*virology

Japanese encephalitis virus (JEV) is the leading cause of viral encephalitis in Asia and domestic pigs serve as the amplifying hosts. In the present study, the full genomic sequences of two JEV strains (HEN0701 and SH0601) isolated from pigs in China were determined and compared with other 12 JEV strains deposited in GenBank. These two strains had an 88.8% nucleotide sequence similarity and 97.9% deduced amino acid sequence homology. HEN0701 had high nucleotide sequence and high amino acid sequence identity with genotype I (GI) strains, while SH0601 had high nucleotide sequence and high amino acid sequence identity with GIII strains at both the gene and full genome levels. Further phylogenetic analysis showed that HEN0701 belonged to the JEV GI group and SH0601 was classified as a GIII strain. Analysis of codon usage showed there were a few differences between the GI and GIII strains in nucleotide composition and codon usage for the open reading frames.
Animals ; Cell Line ; Cricetinae ; Encephalitis Virus, Japanese/classification/*genetics ; Encephalitis, Japanese/epidemiology/*veterinary/virology ; Gene Expression Regulation, Viral/physiology ; Genome, Viral ; Molecular Epidemiology ; Phylogeny ; Swine ; Swine Diseases/epidemiology/*virology

Objective To explore the changes and significane of USP20 and HIF-α expression in breast cancer.Methods Following transfection of shRNA-USP20 lentivirus into breast cancer MDA-MB-231 cells,the gene and protein expression levels of USP20 were detected using fluorescence quantitative PCR and Western Blot.Subsequently,the overexpression of USP20 was observed to determine its effect on HIF-α expression.Similarly,siRNA-USP20 was used to knock down USP20 in breast cancer MDA-MB-231 cells,followed by detection of gene and protein expression levels using fluorescence quantitative PCR and Western Blot.The subsequent changes in HIF-α expression were then examined.Rusults The positive expression rates of USP20 and HIF-α in breast cancer tissues were 69.6%and 46.83%,respectively,while they were negatively expressed in the adjacent normal tissues,with statistically significant differences(P＜0.01).The positive expressions of USP20 and HIF-α were predomi-nantly observed in the cytoplasm of breast cancer tissue,with a smaller amount present in the nucleus.There was a significant positive correlation between USP20 and HIF-α in breast cancer.Following transfection of shRNA-USP20 lentivirus into MDA-MB-231 cells,both the protein and gene expression levels of USP20 significantly increased(P＜0.01).Over-expression of USP20 did not affect HIF-α mRNA levels but led to a significant increase in HIF-α protein expression(P＜0.01).Conversely,siRNA-USP20 interference resulted in a significant decrease in both the protein and gene expression levels of USP20(P＜0.01),without affecting HIF-α mRNA levels;however,it caused a notable reduction in HIF-α protein expression(P＜0.01).Conclusion The expression of USP20 exhib-ited a significant positive correlation with HIF-α in breast cancer.Overexpression of USP20 led to a substantial increase in HIF-α protein expression,while knock-down of the USP20 gene resulted in a significant decrease in HIF-α protein levels.Therefore,it can be inferred that USP20 may exert its influence on the development of breast cancer through modulation of HIF-α expression,thereby providing crucial experimental evidence for clinical treat-ment,prognosis,and further investigations.