control group,and showed statistically significant difference among three groups(P0.05).Conclusion Excessive fluorine intake may cause unbalanced free radical metabolism.It suggests that the intervention on fluorine intake will contribute to resume the balance of oxidation.

[Objective]To investigate the effect of chondroitinase ABC(ChABC) after spinal cord injury(SCI) in adult rats,and to evaluate the significance of treatment. [Methods]Forty adult Wistar rats were divided into two groups: the control group(group A),the chondroitinase ABC group(group B).The thoracic T10 spinal cord injury model was made by impactor model II manual.After SCI,group B animals received ChABC treatment,while group A animals only received blank saline.The locomotor functions(BBB score) and the neuroelectrophysiological changes(SEP&MEP) of the rats were evaluated.Twelve weeks after SCI,biotinylated dextran amine(BDA) was injected into the cerebral cortex to trace the corticospinal tracts(CST).Then two weeks after the tracing,the animals were killed and the spinal cord frozen sections were made.The tissue sections were processed with Cy3 fluorescence stain,HE stain and anti NF-200 immuohistological stain.The section images were analyzed by Image Pro Plus image analyzing software.All the statistics were performed by SPSS 13.0 software.[Results]At 4 weeks after SCI,group B had obvious higher BBB scores than control group(P

[Objective]To investigate the surgical techniques and clinical result of the treatment of subtrochanteric femoral fracture(SFF) with the reconstruction interlocking nail(RIN).[Method]A total of 40 cases(27 males and 13 females,at age ranged from 22 years to 75 years,mean 43.5 years) with SFF were fixed with RIN in our hospital from June 1998 to May 2005.The fractures were classified according to Seinsheimer,the type Ⅱ 5 cases,type Ⅰ 13,type Ⅳ 21 and type Ⅴ 1.33 patients sustained high-energy injury,and 7 low-energy Three cases were open fractures.[Result]The followed-up period ranged from 8 months to 2 years and 6 months with an average of i year and 8 months.The average time for bony union on xray was 4.2 months (from 3.2 to 6.5 months).According to HAN's scoring system there were 26 excellent cases,12 good cases and 2 fair cases.The excellent and good rate was 95%.There was no severe complications,such as incision infection, fixation failure,deformity or ischemic necrosis of femoral head.[Conclusion]RIN is an effective operation for treatment of SFF.This method has obvious advantages of reliable fixation,few complications and the high bone union rate.Meticulous surgical techniques is the key of success for the operation.

Objective This paper will study the effect of the replantation and full crown prosthesis in the treatment of refractory molar periapical disease as well as their related factors .Methods The retrospective analysis will be used in this paper.There were 98 patients who were diagnosed as refractory molar periapical disease in our dental clinic from 2012 to 2015.These 98 cases are divided into two groups:the study group and the control group .This paper will make a comparison of the study group which represents the replantation and full crown prosthesis in the treatment and the control group which re -presents endodontic therapy .Results Through the comparison , the average effective rate of the study group is 93.9%while the control group 65.3%.There is a significant difference between two groups (P<0.01) and the rate of the study group is higher than that of the control group .Conclusion Through the analysis , this paper finds that the joint optimization of replan-tation and full crown prosthesis can provide an unconventional approach to treat refractory periapical of teeth and effectively preserve the tooth .

Objective To study the pathological features of two huge spontaneous tumors in Wistar and GK rats. Methods Forty Wistar rats and 40 GK rats were included in this study. Among those rats, two huge spontaneous tumors were observed in a Wistar rat at 14 months of age and in a GK rat at 22 months of age. The growth and survival status of the tumor?bearing rats were recorded. The tumors were surgically removed, and their pathological features were examined using HE and immunohistochemical staining (vimentin, CK19, α?SMA, CD31, CD34, S?100, NF及Ki?67). Results Both the two tumors were completely resected by surgery without much difficulties, and both host rats survived well after the operation. The weight of the two huge tumors was 502 g and 119 g, which corresponding to 64% and 24% of the body weight of their host rats, respectively. The tumors surface had a complete capsule, with a clear boundary separating from the normal surrounding tissues, and no vascular pedicle structure was found. According to the results of immunohistochemical staining, both the two tumors were diagnosed as benign fibroma. Conclusion This type of huge spontaneous tumors is benign fibroma. Besides the impact on the activities of the rats, the tumors have no significant impact on the living conditions in the hosts.

Objective To investigate the effect of employing achievement management in nursing management of urology department.Method The results of assessments were linked to the achievements by regulating achievement-oriented distribution and assessment methods.Results The degree in patients' satisfaction with nursing service and the score on nursing quality assessment were both significantly improved compared to pre-performance of achievement-oriented distribution and assessment(P<0.01). Conclusion The achievement-oriented management may be effective in mobilizing nurses' working enthusiasm and increase quality of care,which guarantees fundamental nursing care.

Objective To observe the effects of endobronchial decimeter wave therapy on ventilation func-tion of patients with the constrictive endobronehial tuberculosis (EBTB). Methods Forty-four EBTB patients weredivided into an experiment group and a control group. All the patients of the two groups were treated with generalchemotherapy, the patients of experiment group accepted endobronehial decimeter wave therapy at the same time. Thepurulent secretion or caseous necrosis of the diseased region was drawn-off from the bronchi lumina by bronchoseope,then the antenna of the decimeter wave irradiator was inserted into the diseased region through the biopsy pore to per-form the radiation. The vital capacity, forced expiratory volume in the 1st second and maximal voluntary ventilationwere tested before and 60 days after the operation. Results Endobronehial decimeter wave therapy significantly re-duced the constriction of bronchi, improved the lung ventilation function, and relieved the anhelation symptom of pa-tients in the experiment group to a significantly larger extent than those in the controls group (P< 0. 01 ). Moreover,the therapeutic effects of endobronchial decimeter wave therapy were more significant for the endomembrane tuberculo-sis with pulmonary closure due to ulcerous necrosis or granulation hyperplasy ( P < 0. 05 ) , but ineffective for the atro-phy due to fibrous constriction. Conclusion Endobronchial decimeter wave therapy could reduce the incidence oflung closure in endomembrane tuberculosis and improve lung function.

Objective To investigate the incidence of dental fluorosis,urinary fluoride level and intelligence of children who lived in the coal-burning-borne endemic fluorosis areas and to reveal the effects of comprehensive control measures on intelligence of children in this area.Methods Children aged 8-12 who lived in coal-burning-borne endemic fluorosis areas in Bijie City of Guizhou Province were selected and divided into two groups according to the duration of comprehensive treatments given:long treatment group (Xiaba Village and Zhongtun Village,furnace stovewas changed and comprehensive control measure of health education was carried out for more than 3 years) and short treatment group(Chadi Village and Maoliping Village,stoves were improved and health education time ＜ 1 year).The children who lived in a non-fluorosis area were selected as controls in 2012.Dental fluorosis was diagnosed by the method of Dean; urinary fluoride was analyzed by the method of fluoride-ion selective electrode; and the intelligence quotient (IQ) was measured by Raven's Standard Progressive Matrices Test.Results The number of children surveyed in control group was 104,long treatment group was 298,short treatment group was 339,and the incidence rates of dental fluorosis were 0 (0/104),725％(216/298) and 85.2％ (289/339),respectively,and the incidence rates of dental fluorosis in children lived in the endemic fluorosis areas were significantly increased compared with that of control group; the difference of incidence rates between long treatment group and short treatment group was statistically significantly(x2 =15.736,P ＜ 0.01).Urinary fluoride content were (2.33 ± 0.18) and (3.03 ± 0.16)mg/L,respectively,compared with the control group[(1.34 ± 0.64) mg/L],the values in endemic fluorosis areas were significantly higher(F =306.53,P ＜ 0.01).Above average IQ of children in the control group was 97.1％ (101/104),which was significantly higher than that of long and short treatment groups; after a lengthy treatment,mental retardation detection rate was significantly lower in the low-age group,8-10 year-old ehildren(x2 =7.542,P ＜ 0.01).Urinary fluoride content was negatively correlated with the level of IQ (r =-0.553,P ＜ 0.01).Conclusions The intelligence development of children in coal-burning-borne endemic fluorosis area is significantly delayed.After a certain period of comprehensive treatment,the decreased level of cognition is inhibited and the mental retardation in the low-age group is improved.

Objective To investigate the effect of mitochondrial fission on the function of pancreatic β cells.Methods INS-1 stable cell lines allowing inducible expression of either wild-type dynamin-related protein 1 (Drp-1 WT)or its dominant-negative mutant(Drp-1 K38A)were used.The effect of mitochondrial fission on the function of pancreatic β cells were investigated under different concentrations of glucose.Results There were increased mitochondrial fission and disintegration of the mitochondrial reticulum into multiple punctiform organelles in Drp-1 WT cells induced with doxycycline under high glucose condition.Insulin secretion(P<0.01),mitochondrial membrane potential(P<0.05),and ATP content(P<0.05)were decreased and cytochrome C expression was increased after the expression of Drp-1 WT under high glucose condition while these changes were markedly mild in Drp-1 K38A expression cells.Conclusion The increased mitochondrial fission inhibits pancreatic β cell function.

Objective: To observe the therapeutic effect of Jingyuankang capsule on peripheral leukopenia in patients with HIV/AIDS.Methods: The methods of randomized,double blind,double analogue clinical trials were used for two groups: 58 cases treated with Jingyuankang capsule,Likejun tablet analogue and HAART therapy;58 cases treated with Likejun tablets,Jingyuankang capsule analogue and HAART therapy.Both groups had systematic administration of 6 weeks.The peripheral blood cells were examined to observe the therapeutic effect of Jingyuankang capsule on peripheral leukopenia.Results: Jingyuankang capsule had a good therapeutic effect in treating peripheral leukopenia.It signifi cantly increased the numbers of leukocytes and granulocytes.As the time of administration,it could promote the growth of leukocyte more obviously.It was clinical safe and effective.No toxic and side effect was found in the process of treatment and later follow-up visit.Conclusion: Jingyuankang capsule can effectively treat peripheral leukopenia in patients with HIV/AIDS.