Objective To explore the difference of DNA methylation levels between normal Schwann cells (NSCs) and activated Schwann cells (ASCs) in rats. Methods The adult Wistar rats were received sciatic nerve ligation and fed for 7 days. The ASCs and NSCs were separated from ligated sciatic nerves and brachial plexus respectively. Immunocytochemical staining of S-100 antibody was used to identify the cells. The growth condition of cells was detected by CCK-8 method. Methylated DNA immunoprecipitation sequencing (MeDIP-Seq) was applied to filter the differentially methylated regions in ASCs and NSCs. The distribution of differentially methylated genes related with axonal regeneration in chromosome was analyzed, and Gene ontology(GO)and PATHWAY analysis were also conducted. Results High purity of ASCs and NSCs were obtained successfully, which were both positive for S-100 antibody. In the same culture condition, ASCs showed a faster proliferation than that of NSCs. A total of 177 176 differentially methylated regions were found by MeDIP-Seq. Among them, 1 097 were located in the promoter (≤1 kb), 1 136 in the promoter (1-2 kb) and 567 on the CpG. After functional annotation of differentially methylated genes, 214 differentially methylated genes related with axonal regeneration were found in ASCs and NSCs. Compared with NSCs, 191 genes were up-regulated and 23 genes were down-regulated in ASCs. These genes were located on different chromosomes, most of which on chromosome 12 (22 genes) and the least on chromosomes M (2 genes). GO analysis indicated that the differential methylated genes were involved in axon growth, axon formation, axon elongation and axon guidance. The MAPK, cell adhesion molecules, Ras signaling pathway may be related with the differential methylated genes. Conclusion The methylation levels between ASCs and NSCs are significantly different, which are probably related with axon regeneration.

Objective Through the research of the disposal of clinic laboratory result information,the paper explore the methods of building up laboratory's online management system and network information system.Methods This project began with the analysis of the need of laboratory and applies computer to simulate the manual disposal process.The system framework was separated into many sub parts according to its function.VB,etc were used to program.Results The project has been gained successfully in the online managemert of two analyzers and the management of data network consisting of three laboratories.Conclusion The project has established a practical and reliable in-formation management system which matches the work mode in clinical laboratory and has novelty functions.

Objective To evaluate the therapeutic effect of extendable intramedullary nail on children with femoral deformity due to osteogenesis imperfecta.Methods From June 2009 to June 2012,21 patients with femoral deformity due to osteogenesis imperfecta were treated with extendable intramedullary nail.There were 13 males and 8 females,aged from 9 years and 6 months to 15 years and 7 months (average,12 years and 3 months).All children had been performed osteotomy on the shaft of femur and implanted with non-extendable nail before 2-4 years (average,3 years).All children had suffered refracture and deformity,including 9 children with femoral bending deformity and 12 with refracture.The deformity angle ranged from 10°to 30°,with an average of 15°.According to revised Sillence classifications,there were 6 cases with type Ⅲ and 14 with type Ⅳ and 1 case with type Ⅴ.Twenty-one patients were operated with extendable nail for fixing fracture and correcting deformity and incisions were 2-3 cm long and located on the great trochanter and distal osteotomy point.Results All of 21 children were followed up for 6-30 months (average,18 months).The bone healing time was 7-12 weeks (average,8.5 weeks).Patients started to walk after X-ray showing bone union.Parents of 21 children were satisfied with surgical operation effect and deformity correction.The Barthel index score improved from 72.85 (range,50-90 points) preoperatively to 91.42 (range,80-100 points) postoperatively at the latest follow-up of patients.WeeFIM index score increased from preoperative average of 55.42 points (range,40-70points) to postoperative average 79.00 points (range,70-86 points).Ten of all children with stick aid preoperative could walk independently after small incision repairing,and 6 of all children in sickbed preoperative,4 of 6 children could walk independently,2 of 6 children could walk with stick aid.Conclusion Small incision repair with extended intramedullary nail operation therapy is advantaged.It gets less bloody,less damages,less pain,less healing time and walking after removing plaster.

Objective To investigate the protective effect of arbidol on aquaporin-4 (AQP4) antibody-induced neurotoxicity in vivo and in vitro.Methods (1) The cortical cells were conventionally obtained from newborn SD rats;4 days after seeding,the cultured cortical cells were randomly divided into four groups:control group,AQP4 antibody-positive serum treatment group,arbidol preconditioning group and dimethyl sulfoxide (DMSO) treatment group;cells in the arbidol preconditioning group were subdivided into 12.5 μmol/L,25 μmol/L and 50 μmol/L arbidol preconditioning subgroups;cells in the control group were given 10％ volume ratio of healthy human sera;cells in the AQP4 antibody-positive serum treatment group were added the same volume of AQP4 antibody-positive serum;cells in the 12.5 μmol/L,25 μmol/L and 50 μmol/L arbidol preconditioning subgroups were given arbidol one h before adding AQP4 antibody-positive serum.After 6 h of cultivation,the morphological and total area changes of astrocytes and neurons through Hoechst33258/PI staining and immunofluorescence were aboserved.(2) Twenty-four SD rats were randomly divided into control group,AQP4 antibody-positve serum treatment group,arbidol preconditioning group and DMSO treatment group;rats in the later three groups were induced animal models ofneuromyelitis optica (NMO) via intracerebral injection of AQP4 antibody positive serum;rats in the later two groups were given 54 mg/kg (concentration:10 mg/mL) arbidol and DMSO respectively,3 h before adding AQP4 antibody-positive serum;NMO models were identified by HE and immunohistochemical staining.Three d after convention feeding,the rats in the four groups were sacrificed,and the brain tissue sections were performed immunohistochemical staining to detect the expression levels and lesion size of AQP4 and glial fibrillary acidic protein (GFAP).Results (1) In vitro,the cell viability in 25 μmol/L arbidol preconditioning subgroup was significantly increased as compared with AQP4 antibody-positive serum treatment group and other subgroups (P＜0.05).The total areas of positive cells (astrocytes and neurons) in AQP4 antibody-positive serum treatment group were significantly decreased as compared with those in the control group (P＜0.05);while those in the arbidol preconditioning group was significantly increased as compared with those in the AQP4 antibody-positive serum treatment group (P＜0.05).(2) In vivo,intracerebral injection of AQP4-Ab positive serum caused NMO-like pathology,with leukocyte infiltration and loss of AQP4 and GFAP within the lesion;the loss areas of AQP4 and GFAP in AQP4 antibody-positive serum treatment group were significantly increased as compared with those in the control group (P＜0.05);while those in the arbidol preconditioning group was significantly decreased as compared with those in the AQP4 antibody-positive serum treatment group (P＜0.05).Conclusion Arbidol alleviates the neurotoxicity which induced by AQP4-Ab positive serum in vivo and in vitro,and it would be effective on the treatment of NMO.

Osteoporotic thoracolumbar fracture in the elderly will seriously reduce their quality of life and life expectancy. For osteoporotic thoracolumbar fracture in the elderly, spinal reconstruction is necessary, which should comprehensively consider factors such as the physical condition, fracture type, clinical characteristics and osteoporosis degree. While there lacks relevant clinical norms or guidelines on selection of spinal reconstruction strategies. In order to standardize the concept of spinal reconstruction for osteoporotic thoracolumbar fracture in the elderly, based on the principles of scientificity, practicality and progressiveness, the authors formulated the Clinical guideline for spinal reconstruction of osteoporotic thoracolumbar fracture in elderly patients ( version 2022), in which suggestions based on evidence of evidence-based medicine were put forward upon 10 important issues related to the fracture classification, non-operative treatment strategies and surgical treatment strategies in spinal reconstruction after osteoporosis thoracolumbar fracture in the elderly, hoping to provide a reference for clinical treatment.