1.Early Mobilization for Intensive Care Unit Acquired Weakness (review)
Shiqin PAN ; Qing ZHANG ; Li WANG
Chinese Journal of Rehabilitation Theory and Practice 2017;23(1):50-53
Early mobilization is beneficial in the prevention of intensive care unit acquired weakness. This paper summarized the meth-ods, opportunity, evaluation of early mobilization intervention, and some notes.
2.Advance in Modified Constraint-induced Movement Therapy for Upper Extremity Function Recovery after Stroke (review)
Qing ZHANG ; Shiqin PAN ; Li WANG
Chinese Journal of Rehabilitation Theory and Practice 2016;22(12):1395-1398
Modified constraint-induced movement therapy (mCIMT), which is modified from constraint-induced movement therapy (CIMT), is used in hemiplegics for rehabilitation. It may be used in the selected patients in certain function and age. mCIMT is more flexible than CIMT, which is mainly consisted of intervention movement, intensity, and the time of training and limitation. mCIMT is also used with other rehabilitation methods. There are various assessment for the evaluation of upper extremity motor function. By using Contract and Mo-tor Activity Log, researchers can better guarantee the curative effect.
3.One case report of overseas imported quartan malaria
Hua JIANG ; Yongbin WANG ; Ping ZHANG ; Shiqin ZHANG ; Lei YAN
Chinese Journal of Schistosomiasis Control 2014;(4):477-478
This paper reports one case of overseas imported quartan malaria and the diagnosis and treatment process. By using dihydroartemisinin combined with piperaquine,the treatment results are satisfactory.
4.Development of sustained release tablet of tamoxifen citrate and its in vitro release profile
Kuangshi WU ; Ganlin ZHAO ; Shiqin ZHANG ; Qian ZHANG
Journal of Peking University(Health Sciences) 2003;0(06):-
Objective: To reduce the frequency of administration of tamoxifen citrate so as to improve its bioavailability and patients’ compliance. Methods: HPMC K4M was employed as major retarded release controller. The wetting granulation and directly compressing method was used to produce the sustained release tablet. Then the in vitro release profile was applied as main criteria to evaluate six formulations according to the variation of HPMC K4M amount. The concentration of tamoxifen citrate was measured by UV spectrometry. Finally the releasing characteristics of sustained release and conventional tablets were compared to clarify the sustained effect of the former. Result: At 278 nm there was no interaction between tamoxifen citrate and the recipients so that it was adopted as the wavelength of determination. The recovery efficiency of this method ranged from 95%-105%. The final formulation could release 86.40% of its loading amount in 12 h and its releasing profile fitted the Zero order equation well. The percentages of accumulative release in 1 h were 76.81% and 7.08% for sustained release tablet and conventional tablet respectively. Conclusion: The sustained release tablet of tamoxifen citrate could demonstrate a continuous and stable releasing profile and last for over 12 h. It has significant retarded effect in comparison with the conventional one and could be a new choice of regimen in its clinical application.
5.Effect of wire-reinforced epidural catheters on success rate of epidural catheterization for labor anal-gesia
Yao ZHANG ; Shiqin XU ; Xiaofeng SHEN ; Yunhe ZHU ; Caijuan LI
Chinese Journal of Anesthesiology 2016;36(11):1319-1321
Objective To investigate the effect of wire?reinforced epidural catheters on the success rate of epidural catheterization for labor analgesia. Methods A total of 200 nulliparous parturients who re?ceived labor analgesia voluntarily, of American Society of Anesthesiologists physical status Ⅰ or Ⅱ, aged 20-45 yr, with body mass index<35 kg∕m2, were divided into 2 groups (n=100 each) using a random number table: common catheter group ( group Ⅰ) and reinforced catheter group ( group Ⅱ) . After suc?cessful epidural puncture, the corresponding catheter was inserted inⅠandⅡgroups. The development of difficult insertion, intravascular catheter insertion or paresthesia during insertion was defined as a failure of epidural catheterization. The occurrence of the failure of epidural catheterization was recorded. Results Compared with group Ⅰ, the failure rate of epidural catheterization was significantly decreased in groupⅡ( P<0.05) . Conclusion Wire?reinforced epidural catheters can raise the success rate of epidural catheter?ization for labor analgesia.
6.Application of mind mapping on adverse effect caused by medication via PCA
Jirong XIAO ; Tian ZHANG ; Wei MA ; Shiqin LV ; Axue WU ; Xue CHEN
Modern Clinical Nursing 2014;(7):52-54
Objective To evaluate the effect of mind mapping on adverse effects caused by medication via patient control alagesia pump(PCA).Methods One hundred and seventy-nine patients with PCA pump after operation were divided into control group(n=90)and observation group(n=89)according to the admission time.The observation group was nursed with mind mapping and the control group with routine method. The incidences of adverse effects caused by medication via PCA pump were compared between the two groups.Result The incidences of adverse effect in the observation group and the control group were 19.1%and 32.2%respectively,with statistical significance(P<0.05).Conclusion Nursing with mind mapping for medication via PAC pump can improve nurses’clinical ability in observation and nursing and therefore effectively prevent and deal with adverse effect caused by medication via PCA pump to relieve patients’pain and improve nursing quality.
7.Comparison of programmed intermittent epidural bolus with continuous epidural infusion at different time intervals for epidural labor analgesia
Zhaohui WANG ; Shanwu FENG ; Shiqin XU ; Panpan ZHANG ; Nan WANG ; Xiaofeng SHEN
The Journal of Clinical Anesthesiology 2017;33(8):755-759
Objective To investigate the effects of programmed intermittent epidural bolus (PIEB) with continuous epidural infusion (CEI) at different time intervals for epidural labor analgesia.Methods One hundred and eighty-six nulliparous parturients were randomized to the groups P1, P2 and C.Epidural infusion was given initial loading dose: 10 ml (0.125% ropivacaine with 0.4 μg/ml sufentanil), followed by maintaining dose: 0.08% ropivacaine with 0.4 μg/ml sufentanil.Group P1 was given basal infusion 5 ml per 30 min, 30 min after the initial dose;group P2 was given basal infusion of 10 ml per 60 min, 60 min after the initial dose;CEI basal infusion of 10 ml/h immediately after the initial dose;PCEA (patient-controlled epidural analgesia dose) 5 ml (lockout interval: 30 min).The baseline maternal heart rate, noninvasive arterial blood pressure, SpO2, respiratory rate, and fetal heart rate tracing were recorded.The visual analog scale (VAS) was recorded during the first stage of labor and at full cervical dilation.The degree of motor block was assessed in both lower extremities using the modified Bromage score (MBS).The maximum blocked segment, the consumption of anesthetic, delivery mode, amount of oxytocin, The number of cases of motor block and intrapartum fever, the fetal Apgar scores,adverse reactions,maternal satisfaction score were recorded.Results The VAS obviously decreased in the three groups since receiving labor analgesia (P<0.05).The ratio of VAS score more than 3 scales in group PIEBⅡ was significantly lower than that in groups P1 and C (P<0.05).The maximum blocked segment increased in group P1, while The incidence of unilateral block was significantly decreased in group P2 (P<0.05).The total drug consumption.And the toatal number of PCA were decreased obviously in group PIEB (P<0.05).The initial PCA time was significantly prolonged in group P2 (P<0.05).The number of instrumental midwifery and intrapartum fever in group P2 were significantly lower than that in group C (P<0.05).Conclusion Programmed intermittent epidural bolus at the beginning 60 min intervals after the initial dose for epidural labor analgesia is scientific and effective.
8.Effect of total flavonoids of Scutellaria baicalensis Georgi on expression of influenza A virus nucleoprotein in HeLa cells.
Qing ZHANG ; Bin YANG ; Nongrong WANG ; Linjian DUAN ; Shiqin HE ; Jian SUN
Journal of Southern Medical University 2012;32(7):966-969
OBJECTIVETo investigate the effect of total flavonoids of Scutellaria baicalensis Georgi (TFSB) on exogenous expression of influenza A virus nucleoprotein (NP) in HeLa cells.
METHODSHeLa cells were transiently transfected with the empty vector pcDNA3.1(+) or pcDNA3.1(+)/NP vector harboring influenza A virus NP. The pcDNA3.1(+)/NP-transfected cells were treated with TSFB and the expression of influenza A virus NP in the cell supernatant was measured using colloidal gold immunochromatography 48 h after the transfection; fluorescent quantitative RT-PCR was performed to measure the starting copy number of NP gene.
RESULTSThe cells transfected with pcDNA3.1 (+)/NP with and without TFSB treatment were positive for NP expression. Fluorescent quantitative RT-PCR showed that the starting copy number of NP gene in pcDNA3.1(+)/NP-transfected cells was (8.90±2.53)×10⁶ copies/µl, showing no significant difference from that of (6.15±1.49)×10⁶ copies/µl in pcDNA3.1(+)/NP-transfected cells with subsequent TFSB treatment (P>0.05).
CONCLUSIONTFSB treatment does not obviously affect exogenous influenza A virus NP gene expression or its protein synthesis in HeLa cells.
Flavonoids ; pharmacology ; Gene Expression ; HeLa Cells ; Humans ; RNA-Binding Proteins ; biosynthesis ; genetics ; Scutellaria baicalensis ; Transfection ; Viral Core Proteins ; biosynthesis ; genetics
9. The effective application of ventilation at prone position on patients with severe acute respiratory distress syndrome at high altitude
Xiaolin SUN ; Shiqin PAN ; Hao WANG ; Jinhai HAN ; Xia ZHANG
Chinese Journal of Practical Nursing 2019;35(9):699-703
Objective:
To explore the effective application of ventilation at prone position on patients with severe acute respiratory distress syndrome(ARDS) at high altitude.
Methods:
A total of 19 severe ARDS patients in ICU of Qinghai Province People's Hospital were enrolled from February in 2016 to February. in 2018, all of them ventilated at the prone position, several indicators were assessed:heart rate (HR), respiratory (R), mean arterial pressure (MAP); blood gas indicators: arterial oxygen partial pressure (PaO2), inhaled oxygen concentration (FiO2), carbon dioxide partial pressure (PaCO2), blood oxygen saturation (SpO2), oxygenation index (PaO2/FiO2), PH, lung compliance, PEEP and so on, which assessed before and 12h, 24h, 48h, 72h after the ventilation.
Results:
FiO2 and PEEP significantly declined at 12 h, 24 h, 48 h, 72 h after ventilation at the prone position. HR, MAP, R, PaO2, PaCO2, SPO2, PaO2/FiO2, pH and lung compliance significantly improved (
10.Effect of dauricine on apoptosis and expression of apoptogenic protein after transient focal cerebral ischemia-reperfusion injury in rats.
Xiaoyan YANG ; Li ZHANG ; Shiqin JIANG ; Peili GONG ; Fandian ZENG
China Journal of Chinese Materia Medica 2009;34(1):78-83
OBJECTIVETo investigate the effect of dauricine on the apoptosis of neuronal cells and the expression of apoptosis-related proteins in the brain penumbra of rats induced by transient focal cerebral ischemia-reperfusion injury.
METHODMale SD rats were randomly divided into five groups: sham group (Sham), model group (Model), and Dauricine groups of low, middle and high doses. To make the transient focal cerebral ischemia-reperfusion injury model, the middle cerebral artery on the right side of rat was occluded by inserting a nylon suture through the internal carotid artery for 1 h, followed by reperfusion for 24 h after withdrawing the suture. Dauricine groups, different doses of Dauricine (2.5, 5, 10 mg x kg(-1) as low, middle and high dose respectively) were administered intraperitoneally at the beginning of the cerebral ischemia, and at 11 h and 23 h after reperfusion. At the same time, Sham group and Model group was administered saline as controls. Brain samples of rats were treated with paraformaldehyde perfusion fixation 24 h after blood reperfusion and then collected for making pathological sections. Apoptotic changes of neuronal cells in the brain penumbra of rat were evaluated in situ by terminal deoxyribonucleotidyl transferasemediated dUTP-digoxigenin nick end-labelling (TUNEL). Cytochrome C (Cyt-C) release and the expression of caspase -3 and caspase -9 proteins of the ischemic-reperfusion brain tissue were determined by immunohistochemistry assay.
RESULTTUNEL-positive cells in groups of middle and high doses of dauricine (18.9 +/- 2.02 and 15.9 +/- 2.9 cells/mm2 respectively) decreased significantly compared with model group (25.5 +/- 3.3 cells/mm2, P<0.05). Cyt-C release and the expression of caspase-3 and caspase-9 proteins in groups of middle and high doses of dauricine were also inhibited compared with Model group (P<0.01).
CONCLUSIONThe mechanism of the neuroprotective effect of dauricine after cerebral ischemia-reperfusion injury may parly, related with an inhibition of neuronal cells apoptosis in the penumbra.
Animals ; Apoptosis ; drug effects ; Benzylisoquinolines ; pharmacology ; Caspases ; metabolism ; Cytochromes c ; metabolism ; Dose-Response Relationship, Drug ; Gene Expression Regulation ; drug effects ; Ischemic Attack, Transient ; surgery ; Male ; Neuroprotective Agents ; pharmacology ; Rats ; Reperfusion Injury ; metabolism ; pathology ; prevention & control ; Tetrahydroisoquinolines ; pharmacology