Objective:To study the disturbance creatd by the variable transformation within regression parameters of nonlinear biology models and find a new method removing or reducing the disturbance.Methods:To use the space transformation,Taylor series and weight method to remove or reduce the serious disturbance created by the variable transformation within regression parameters of nonlinear biology models.Results:Giving a new method calculating regression parameters of nonlinear biology models.Conclusion:The new method can remove or reduce the serious disturbance created by the variable transformation and remain the advantage of variable transformation.It can not only improve regression precision of the nonlinear biology models but also can find problems hidden in the original data.

Objective Research on variable substitution to non-linear regression forecast model precision's influence, and seek the modelling method that can improve the forecast precision. Methods Based on the data mining,the transform in space and the weighted processing combined method, make full use of information that the primary data provide. Results Given modelling method of combination forecast model based on the data mining. Conclusion Based on data mining's combination forecast model's modelling method can reduce the serious influence that the variable substitution brings and has fully used useful information in the primary data. It obviously improved the accuracy of the prediction model.

Objective To investigate the expression of Annexin A1 proteins in colorectal cancer tissues and the relationship be-tween it and clinicopathologic features of colorectal cancer.Methods The surgical specimens from 48 cases of colorectal cancer,36 cases of adjacent tissues,15 cases of adenoma polyp were used to be examined expression of Annexin A1 immunohistochemical method.Results The positive expression rate of Annexin A1 in cancer tissue(64.6%)was significantly higher than those in adja-cent tissues(38.8%)and adenoma polyp tissues(40.0%)(P<0.05).The adjacent tissues and adenoma polyp tissues with positive Annexin A1 expression showed mild to severe atypical hyperplasia.The positive rates of Annexin A1 were significantly lower in the well-differentiated adenocarcinoma,tumor size of <5 cm,with no metastasis to lymphnodes and no invasion to surrounding tissues of colorectal cancer than those in the moderately or poorly differentiated adenocarcinoma,tumor size of ≥5 cm,with metastasis to lymphnodes and invasion in surrounding tissues(P<0.05).Conclusion The expression levels of Annexin A1 may be an important index of occurrence,progression and biological behaviors of colorectal carcinoma.

Objective To explore the feasibility and safety of systemic chemotherapy concurrent whole brain radiotherapy (WBRT) for non-small cell lung cancer (NSCLC) with brain metastases.Methods Eighty cases of NSCLC patients with brain metastases were divided into observation group (40 cases) and the control group (40 cases) according to random number table.Patients were given systemic chemotherapy synchronous WBRT or sequential WBRT.Results The Ⅰ-Ⅳ degree leukocytopenia incidences of the two groups were 12.5％,25.0％,25.0％,0.0 and 30.0％,25.0％,12.5％,15.0％,and there was statistical significance (x2 =12.12,P ＜ 0.05).In the observation group,the total remission rate was 20％ (8/40),and it was 22.5％ (9/40) in the control group,with no significant difference (x2 =1.79,P ＞ 0.05).The median progression free survival of the observation group and of the control group were (3.5 ± 2.3) months and (3.6 ±1.1) months,respec-tively,with no significant difference (t =5.23,P ＞ 0.05).But the 1-years survival rate in the observation group was 37.5％ (15/40),significantly higher than that in the control group (17.5％,7/40),which had statistical significance (x2 =9.11,P ＜ 0.05).Conclusion The safety of systemic chemotherapy synchronous WBRT for NSCLC patients with brain metastases is higher,with good curative effect and strong application feasibility.

Objective To observe the clinical effect of combined treatment of TCM and western medicine with Ehong plate on UA with different syndrome types, and to investigate the correlation of different syndrome types UA with IL-6, IL-18 and hs-CRP, to search the objective and quantitative indexes for diagnosis and Chinese medicine syndrome differentiation of UA. Methods One hundred and twenty patients were randomly divided into treatment group and control group, both with 60 cases. IL-6, IL-18 and hs-CRP of the two groups before and after treatment were observed. Result The two groups both alleviated UA, the total effective rate of the treatment group and the control group was 97% and 83% respectively (P

Objective To study the relationship between the microvessel damage and the death rate of tumor cells and determine role of microvessel damage in the tumoricidal effect of hyperthermia.Methods H22 hepatoma cells were inoculated in the right hind legs of KM mice with immunosuppression.Local hyperthermia was administered to these mice which were divided randomly into 10 groups (8 in each group),which included groups of 3,6,12,24,48,72 and 96 h after hyperthermia for 30 min and groups of hyperthermia for 15,30 and 45 min.The tumor tissues were analyzed by flow cytometry(FCM)and immunohistochemistry.The cultured H22 cells were also given hyperthermia for 15,30,45 min and analyzed by FCM.Results The apoptotic rate(0.115±0.008)was higher in the initiation phase of hyperthermia in vitro,then the necrotic rates rose gradually.The difference between in vivo and in vitro was significant in hyperthermia of the same time about death rate(P＜0.05).A strong positive linear correlation(r=0.844)was observed between the death rate of tumor cells and MVD.Conclusion Microvessel damage plays the cardinal role in tumoricidal effect of hyperthermia.

Objective To evaluate the clinical value of arterial chemotherapy and embolization in the treatment of advanced uterine carcinoma.Methods In this study,52 cases of uterine carcinoma(including 43 carcinoma of cervix and 9 cases carcinoma of endonetrium)were treated interventional therapy.In these cases,after one or two times interventional therapy and after 10-14 days,45 cases followed by uterectomy and pelvic lymphadenectomy. 7 cases that can't operated continue followed by interventional therapy. Results Curative effect was obvious,the clinical symptoms were alleviated,the bleed in operation was decreased and the tumor was divided easily.In 45 cases followed by operation,the one-year and three-year survival rate are 100% and 92%.In 7 cases treated by interventional therapy alone,the one-year and three-year survival rate are 100% and 85%.Conlusion The interventional therapy preoperation can reduce the clinical stage and metastasis of lymph,improve the ectomy by operation and survival rate of patients obviously,so it can be used as a routine treatment in uterine carcinoma.

Objective To evaluate the effect of isoflurane post-conditioningon the expression of pro apoptotic proteins in the conical neurons exposed to oxygen-glucose deprivation and restoration (OGD/R) in rats.Methods Primary cortical neurons isolated from male Sprague-Dawley rats (within 24h after birth),were cultured in vitro and inoculated in 6-well culture plate (2 ml/well) at a density of 1 × 106/ml.The cells were divided into 3 groups (n =12 each) using a random number table:control group(group C),OGD/R group,and isoflurane post-conditioning group (Ⅰ group).In OGD/R group,the cells were incubated in glucose-free BBS aerated with 95 ％ N2 for 30 min followed by restoration of 2-glucose supply for 1 h.At 24 h of incubation,the cells were collected for detection of neuronal apoptosis (.using Hoechst/PI staining),caspase-3 expression (by Western blot),expression of Bid,Bim and Puma mRNA (by PCR),and expression of Bid,Bim and Puma (by Western blot).Apoptosis rate was calculated.Results Compared with S group,the apoptosis rate was significantly increased,and the expression of caspase-3 and Bid,Bim and Puma mRNA and protein was upregulated in OGD/R group.The apoptosis rate and expression of caspase-3 and Bid,Bim and Puma mRNA and protein were significantly lower in Ⅰ group than in OGD/R group.Conclusion Isofluranepost-conditioning inhibits apoptosis in the cortical neurons exposed to OGD/R through down-regulating pm-apoptotic proteins in rats.

Objective To analyze the clinical characteristics and to screen for causative mutations in CRX and GUCY2D genes in children with cone or cone-rod dystrophy. Methods Clinical data and genomic DNA was collected from 18 children with cone or cone-rod dystrophy, aged from 4 months to 8 years. The coding sequence of the cone-rod homeobox (CRX) gene and two exons of the retinal-specific guanylate cyclase GUCY2D gene (exons 2 and 8) were analyzed by using polymerase chain reaction(PCR) and heteroduplex combined with single-strand conformational polymorphism (heteroduplex-SSCP) analysis. Results All of the 18 patients manifested obvious visual impairment. Nystagmus, photophobia and mild ocular fundus changes were found in 13, 8,and 7 cases respectively. Normal fundus was seen in 11 cases. The visual acuity was less than 0.3 in 4 cases and was unable to measure in the other 14 cases because they were too young. Clinical ocular manifestations between cone and cone-rod dystrophy were overlapped. Mutation in the CRX and GUCY2D genes was not detected in the 18 children with cone and cone-rod dystrophy. Conclusion Visual impairment appeared more early and obvious than fundus changes in children with cone or cone-rod dystrophy. Mutation in the CRX gene may not contribute to this series of patients with cone and cone-rod dystrophy.

Objective To investigate appropriate use of radiofrequency thermal ablation (RFA) in treatment of liver cancer.Methods Fouty-one patients with hepatic cancer were entered into two trials. The liver tumors were treated with percutaneous radiofrequency ablation (PRFA) or intraoperative radiofrequency ablation (IRFA). All patients were followed up postoperatively to assess complications, treatment response, and local recurrence of tumor.Results PRFA and IRFA were performed on 32 tumors in 18 patients and 43 tumors in 23 patients, respectively. Treatment-related complications occurred in 6 of the 18 patients treated with PRFA (33.3%). In contrast, there was only 1 complication after IRFA (4.3%, P