Objective To study the clinical features of acute pancreatitis (AP) in elderly patients. Methods The elderly AP patients from January 1993 to October 1999 in our hospital were studied retrospectively. Results There are 83 cases with a male to female ratio of 1 5∶1 and a median age of 72 7 years.AP was diagnosed in 83 cases for 107 times which included 80 times of mild AP and 27 times of severe AP 13 patien with APts were diagnosed for more than one times.Abdominal pain was the primary symptom in 94 4% patients. 6 patients had no pain 53 0%(44/83) and 84 6%(11/13) patients had cholelithiasis and recurrent AP respectively.60 5% and 72 6% patients were diagnosed by type B ultrasonic and CT respectively . Conclusions In elderly AP patients,abdominal pain was the primary symptom, but a part of patients had no typical symptoms.Cholelithiasis including biliary sludge was an inducement especially in recurrent AP patients. Type B ultrasonic and CT examination were important means in diagnosis.

Objective To determine the distribution of various epilepsies and epileptic syndromes in the epileptic population treated in epilepsy clinics. Methods Totally 1191 patients with seizures visited between April and September, 2004 in epilepsy center of our hospital were analyzed based on International League Against Epilepsy (1989, ILAE) Classification. Results Of the 1191 cases, partial epilepsies and syndromes were 766 cases (64.3%), generalized epilepsies and syndromes were 240 cases (20.2%), epilepsies and syndromes undetermined whether partial or generalized were 79 cases (6.7%), special syndromes were 16 cases(1.3%),other non-epileptic disorders were 90 cases (7.6%). Symptomatic cases were dominant in partial epilepsies and syndromes, and idiopathic cases were dominant in generalized epilepsies and syndromes. Conclusions Most of epileptic patients might be classified accurately. Treatment should be administered as earlier as possible. The distribution of various epilepsies and epileptic syndromes is regular.

Objective To study the expression of APE/Ref-1 in gastric cancer. Methods Detection of APE/Ref-1 protein was performed with gastric cancer tissue microarray (TMA) by IHC in gastric cancer, non-neoplastic mucosa and lymph node with metastasis. Results The positive rates of APE/Ref-1 in cytoblast in non-neoplastic mucosa, tumor tissue, and metastatic lymph nodes were 96.6%, 97.1% and 98.9%, respectively, while the positive rates in cytoplasm were 71.8%, 21.4% and 10.0%, respectively and in both cytoblast and cytoplasm were 71.4%, 21.4% and 10.0%, respectively. Compared to non-neoplastic mucosa, tumor lesion had lower APE/Ref-1 expression in cytoblast and cytoplasm. In 35.9% of patients it was decreased slightly and in 11.2% of them it was decreased markedly in cytoblast (P

Dual-energy X-ray absorptiometry(DEXA),scanning electron microscopy,and bone biomechanical test were used to assess comprehensively bone quantity and quality of ovariectomized rats. In OVX rats,not only bone mineral density (BMD) of lumbar vertebrae in vivo and vitro,but also BMD of femora (except for R3 region) and proximal metaphysis(R1 region) in vitro decreased obviously (P<0.01)，whose bone loss rates of L5 and L6 were the highest and achieved 13%; the trabeculae of OVX rats were few，fine, and discontinued and there were lacunae on the surface;in OVX rats,both compressive strength of vertebral bodies and the mechanical properties of femora decreased;the falling degree of the former was greater;the maximal compressive power of lumbar vertebrae decreased with 33.32%. We conclude that after 4-month post-ovariectomy, the bone quantity and quality of six-month-old rats decreased, especially in the area of abundant cancellous bone such as vertebral bodies,distal femora; the assessment of the efficiency of new drugs to prevent and treat postmenopausal osteoporosis using rat models should include these drugs' effects to the bone mass, the bone structure, and the bone strength.

PurposeTo evaluate the impact of filtered back-projection (FBP) and sinogram-affirmed iterative reconstruction (SAFIRE) technique on the image quality of routine dose temporal bone high resolution CT (HRCT) scan, and to provide reference for the realization of low dose scanning.Materials and Methods Sixty patients underwent routine dose temporal bone HRCT scan, 6 groups of images were reconstructed with FBP and SAFIRE (strength grade 1-5), average CT value, noise (SD), signal to noise ratio (SNR), contrast to noise ratio (CNR), subjective score and lesion detection were compared between different groups.Results There was no significant difference of average CT value among the 6 groups (P>0.05); compared with FBP, SD of SAFIRE (strength 1-5) reconstruction decreased gradually (F=1.78,P<0.01), which was 23.1%, 39.2%, 42.4%, 54.1% and 61.2% respectively; SNR and CNR increased gradually (F=1.42 and 3.15, P<0.05), SNR increased 26.6%, 40%, 71.1%, 117.7% and 153.3% respectively, while CNR increased 17.4%, 33.1%, 63.2%, 104.1% and 147.2% respectively. The differences among the 6 groups were statistically significant (P<0.05), subjective scores of SAFIRE 2-4 were higher than those of FBP, SAFIRE 1 and SAFIRE 5 group, while the difference among SAFIRE 2-4 group was statistically insignificant, reconstruction smoothness and edge sharpness of SAFIRE 3 group was better, with highest objective score.Conclusion Routine dose temporal bone HRCT scan combined with SAFIRE reconstruction can increase the image quality of temporal bone high resolution scan effectively, with the potential for radiation dose reduction.

To localize and characterize the hypothetical protein CT358 in the chlamydial infected cells. CT358 gene from the Chlamydia trachomatis (C. trachomatis) serovar D genome was amplified and cloned into the pGEX and pDSRedCI vectors. The recombinant plasmid pGEX-CT358 was constructed and expressed as GST fusion proteins. The GST-CT358 fusion protein was used to immunize mice to raise the antibodies, which specifically recognized CT358 without eross-reacting with other unrelated proteins. The antibodies were then used to localize the endogenous CT358 protein and determine the expression pattern in Chlamydial infected cells using an indirect immunofluorescence assay (IFA). Meanwhile, pDSRedC 1-CT358 recombinant plasmid was transfected to HeLa cells to evaluate the effect of CT358 expression on the subsequent chlamydial infection. The hypothetical protein CT358 was identified in the inclusion membrane of C. trachomatis-infected cells for the first time,and it was detected as early as 12 h after C. trachomatis infection and remained in the inclusion membrane throughout the rest of the infection cycle. Cytosolic expression of CT358 via a transgene failed to affect the subsequent ehlamydial infection. These observations together have demonstrated that CT358 is a newly identified chlamydial inclusion membrane protein, giving the potentially importance for further understanding the mechanisms of chlamydial intracellular parasitism.

Objective To explore how to reasonably deploy the wounded and sick region on the medical evacuation ship.Methods Three models of the wounded and sick region were set up according to different demand.Simulated exercises were imitated and medical staff and experts selected reasonable models.Results Most medical staff and experts selected the model which deployed the wounded and sick region according to whether the condition of an injury should be treated.Conclusion It is scientific and reasonable that the wounded and sick region is deployed according to whether the condition of an injury should be treated.

OBJECTIVE:To establish a method for the content determination of ephedrine hydrochloride in Luofu mountain rheumatism plaster. METHODS:HPLC was performed on the column of Eclipse XDB-C18 with the mobile phase of acetoni-trile-0.1%phosphoric acid(9∶91,V/V),at the flow rate of 1.0 ml/min. The detection wavelength was 207 nm,the column tempera-ture was 40 ℃,and the volume was 5 μl. RESULTS:The linear range of ephedrine hydrochloride was 81.6-408 ng(r=0.999 7);RSDs of precision,stability and repeatability tests were lower than 2%;and the average recovery rate was 96.73%(RSD=1.9%, n=6). CONCLUSIONS:The method is fast and accurate,and can be used for the content determination of ephedrine hydrochlo-ride in Luofu mountain rheumatism plaster.

To localize and characterize the hypothetical protein CT358 in the chlamydial infected cells.CT358 gene from the Chlamydia trachomatis(C.trachomatis) serovar D genome was amplified and cloned into the pGEX and pDSRedC1 vectors.The recombinant plasmid pGEX-CT358 was constructed and expressed as GST fusion proteins.The GST-CT358 fusion protein was used to immunize mice to raise the antibodies,which specifically recognized CT358 without cross-reacting with other unrelated proteins.The antibodies were then used to localize the endogenous CT358 protein and determine the expression pattern in Chlamydial infected cells using an indirect immunofluorescence assay(IFA).Meanwhile,pDSRedC1-CT358 recombinant plasmid was transfected to HeLa cells to evaluate the effect of CT358 expression on the subsequent chlamydial infection.The hypothetical protein CT358 was identified in the inclusion membrane of C.trachomatis-infected cells for the first time,and it was detected as early as 12 h after C.trachomatis infection and remained in the inclusion membrane throughout the rest of the infection cycle.Cytosolic expression of CT358 via a transgene failed to affect the subsequent chlamydial infection.These observations together have demonstrated that CT358 is a newly identified chlamydial inclusion membrane protein,giving the potentially importance for further understanding the mechanisms of chlamydial intracellular parasitism.

The heptadecatungstodiphosphate substituted by L a(Ⅲ) with the formula　α2-K7P2W17O61(La.OH2 ) (P2W 17La) was prepared by vacant refilling method. The anion of single substitu ted Dawson-type tungstophosphate salt was doped into the polypyrrole (PPY) film on a glassy carbon electrode (GC) by electrochemical method to prepare the chem ically modified electrode (P2W17La/PPY/GC). It maintains not only t he electrochemical activity and electrocatalytic property of P2W17L a, but also has good stability and sensitivity. In 0.50 mol/L HCl solution, th e first cathodic peak of P2W17La doping in polypyrrole film can cat alyze well the electroreduction of nitrite. The catalytic current was proporti onal to the concentration of nitrite between 6.0×10-5 and 1.0×10-2 　mol/L.