Objective To establish a method for simultaneous determination of 12 kinds of organophosphorus pesticides in water. Methods Organophosphorus pesticides in the water were extracted by liquid-liquid extraction, then analyzed with DB-1701 capillary column. In the same time, they were determined with GC by controlling the temperature and the speed of nitrogen. Retention time of the peaks was used for qualitative analysis, while external standard method was used for the quantitative analysis. Results The linear ranges of organophosphorus pesticides were 0.2～50.0 ?g/L, r≥0.999 1. The lowest detection limit was 0.009～0.04 ?g/L, the rate of recovery were 78.4%～93.9% and RSDs were 3.4%～7.5%. Conclusion This method is simple, rapid, sensitive and can efficiently separate and determine 12 kinds of organophosphorus pesticides in the water with 32.5 minutes of operation only.

Insulin resistance induces multiple metabolic disorders and are the common risk factors of diabetes mellitus and cardiovascular diseases.Since the causes are complex,the medical management should be integrated to treat insulin resistance.Lifestyle change is the first choice,and the appropriate agents can be selected if needed.

Objective To explore the efficacy of treating primary dysmenorrheal with modified Shenghua decoction plus western medicine. Methods 120 patients with primary dysmenorrhea were randomly recruited into a control group and a treatment group, with 60 patients in each group. The control group was treated with prostaglandin synthetic inhibitor, and the treatment group was treated with modified Shenghua decoction on the basis of control group. Results The total effective rate of the treatment group and the control group was 90% and 73.3%, respectively, showing significant difference (X2=4.011, P <0.05 ) . Conclusion Modified Shenghua decoction plus western medicine is effective in treating, primary dysmenorrheal.

It has been demonstrated by animal and human experiments that the growth promoting effect of soybean milk substitute formula 5410 is comparable with that of human milk or cow's milk formula commonly used in infant feeding. It was assumed that such a favorable effect is due in part to the inclusion of 5% of egg yolk powder in the formula. However, such an assumption remains to be confirmed.In the present animal experiment, the 5% egg yolk powder was either removed (diet 5700), or substituted with 5% whole egg powder (diet 5411), 5% whole cow's milk powder (diet 5720), 10% whole cow's milk powder (diet 5721).It was fouad that the growth promoting effect of diet 5411 is the, same as that of 5410, while that of the other, diets are all significantly inferior than that of 5410. The role played by the definite amount of egg yolk in the soybean milk substitute is postulated.

AIM　To study the synthesis and anticancer activity of 3-［(3′-methyl-4′-(substituted phenyl)-1′,3′-butadienyl］ indole derivatives. METHODS　Electrophilic-substitution, aldol-condensation, selective-reduction, phase-transfer Wittig reaction and hydrolysis reaction were used in the synthesis of the title compounds. RESULTS　Eleven compounds of 3-［(3′-methyl-4′-(substituted phenyl)-1′,3′-butadienyl］ indole were synthesized. They are new compounds. Compound 8 showed different inhibitory effects on HL-60, HCT-8 and Bel7402 cell lines in vitro, and its inhibitory rate of antiinflammation was 100％ at 10-5 mol*L-1 concentration. CONCLUSION　Compound 8 showed high anticancer and antiinflammatory activities, and is worth further studying.

Objective To investigate the protective effect of ginsenoside Rb1 and Re on injury of the neonate rat cardiomyocyte induced by aconitine alkaloids.Methods The influence of aconitine and ginsenoside Rb1,Re together in neonate rat cardiomyocyte was observed respectively.The influence of these components in neonate rat cardiomyocyte was examined by myocard zymogram.The expression of Ca2+ channel gene Cav1.2 mRNA of neonate rat cardiomyocyte after treatment was observed by RT-PCR.Results The ginsenoside Rb1 and Re were able to decrease the release of AST and LDH,reverse Cav1.2 mRNA abundance induced by aconitine.Conclusion The ginsenoside Rb1 and Re which together with aconitine can alleviate the side effects and boost up the therapeutic effects of aconitine.The action mechanism may be relation with that the ginsenoside Rb1 and Re can decrease injuries of the neonate rat cardiomyocytes and abundant expression of Cav1.2 mRNA induced by aconitine.

Objective To summarize the experience of laparoscopic appendectomy(LA).Methods A retrospective analysis was carried out on clinical data of 162 cases of laparoscopic appendectomy in this hospital from August 2002 to April 2005.Results Laparoscopic operation was completed in all the cases but 1 case of intestinal tuberculosis.The operative time was 30~90 min(mean,42 min) and the intraoperative blood loss was 5~10 ml.No complications were noted.Conclusions Laparoscopic appendectomy has advantages of little invasion,less blood loss,rapid recovery,and fewer complications,being a safe and ideal surgical alternative.

OBJECTIVE:To explore the effects of Xinnaoxin pills on CAT score,cardiopulmonary function and hemorheology of patients with pulmonary heart disease complicating with coronary heart disease. METHODS:In retrospective study,80 cases of pulmonary heart disease complicating with coronary heart disease were randomly divided into control group and observation group with 40 cases in each group. Control group was given routine treatment as relieving asthma,eliminating phlegm and oxygen inhala-tion,anti-infective treatment,correcting acid-base balance,cardiotonic and diuretic treatment. Observation group was additionally given Xinnaoxin pills 1.0 g/time,bid after meal,on the basis of control group. CAT score,cardiopulmonary function and hemorhe-ology index were compared between 2 groups before and after treatment as well as ischemic ECG improvement effect and the occur-rence of ADR after treatment. RESULTS:After treatment,hematocrit hematocrit had changed slightly among hemorheology index-es,and CAT score,other hemorheology indexes and cardiopulmonary function indexes were improved significantly;the improve-ment of observation group was better than that of control group,with statistical significance(P<0.05). Total effective rate of isch-emic ECG was 90.0% in control group and 92.5% in observation group,there was no statistical significance(P>0.05). There was no statistical significance in the incidence of ADR between 2 groups (P>0.05). CONCLUSIONS:Xinnaoxin pills can effectively improve CAT score and cardiopulmonary function of patients with pulmonary heart disease complicating with coronary heart dis-ease. It also can improve hemorheology and shows good clinical efficacy,but great importance should be attached to the safety of drug use in the clinic.

Objective To investigate the cholesterol-reducing effects and tolerance property of Lactobacteria isolated from Kefir grain in vitro. Method In imitating gastroenteric environments,such as low pH (2.0～4.0) and high bile salt concentration (0.2%-0.4%),the survival counts of Lactobacteria was carried out and cholesterol-reducing effects were studied. Results The strains of KTX,KS4 and KL1 had more survival counts in eaperimental condition and KTX. KS4 were isolated as more tolerant strains to gastro-enteric environments and could reduce cholesterol in vitro. Conclusion Lactobacteria isolated from Kefir grain had tolerant properties and cholesterol-reducing effects in vitro.

Objective To explore clinical curative effect of microsurgery of petroclival tumors by transpetrosal presigmoid approach. Methods A series of 23 patients of petroclival tumors resected by microneurosurgery were analyzed retrospectively, including tumors histological types, clinical and preoperative MRI features, operative approaches, surgical technique and common postoperative complications. Results Total resection of the tumor was achieved microsurgically in 16 cases, subtotal resection in 4 cases, partial resection in 3 cases. No patient died in this series. 8 patients presented worsening crainal nerves palsies postoperatively. CSF leaks were uncommon, occuring in 2 patients. Conclusion The tumors in petroclival region can be totally resected by transpetrosal presigmoid approach. The advantages were offered by this approach such as the temporal lobes and cerebellum are minimally retracted; the operative distance of petroclival tumors is shortened with exposure of clivus and juxta-clival region.