1.Application of i-gel laryngeal mask combined with T-seal adapter in painless bronchoscopy
The Journal of Practical Medicine 2015;31(15):2483-2485
Objective To observe the clinical effect of controlled ventilation with i-gel laryngeal mask airway in painless bronchoscopy. Methods 80 patients were randomly divided into control group (group A, n =40) and surface anesthesia group (group B, n = 40). Fent-anyl, ruifentanyl, propofol, succinylcholine anesthesia and controlled ventilation was adopted by i-gel laryngeal mask combined with T-seal adapter in control group.Surface of lidocaine anesthesia, spontaneous breathing, nasal cannula oxygen were applied in surface anesthesia group. Patients vital signs change in bronchoscopy , endoscopic related adverse reactions such as body movement, cough, laryngospasm. anesthesia related adverse reactions such as postoperative bucking, choking hoarseness, examination time and patient satisfaction were recorded. Results Controlled ventilation with i- gel laryngeal mask shows airway changes are small (P < 0.05), and less adverse reaction (P < 0.05). Patients showed higher satisfaction (P < 0.05). Conclusion controlled ventilation with i-gel laryngeal mask in painless bronchoscopy is safe , controls the airwayeffectively , and solve the problem of respiratory depression , which proves to be a feasible method.
2.To construct a new effective supervision system for the clinical school teaching
Chinese Journal of Medical Education Research 2002;0(01):-
Clinical teaching supervision system exhibited several problems in the imple-mentation process such as the team of supervision being not reasonable enough and the connotation of supervision not extensive and not going far in depth enough and so on. Through the way of strengthening the team of the clinical teaching construction,expanding the connotation and improving the functions of supervision,we can construct a new effective supervision system for clinical school teaching.
3.Neoadjuvant Chemotherapy for Potentially Resectable Gastric Cancer
Shiming XIAO ; Chen WU ; Huaiwu JIANG
Chinese Journal of Bases and Clinics in General Surgery 2008;0(08):-
Objective To summarize the current value of neoadjuvant chemotherapy(NAC) for potentially resectable gastric cancer.Methods The recent 5-year literatures searched through the PubMed with the key words:stomach neoplasm,gastric cancer/carcinoma,neoadjuvant therapy/chemotherapy and preoperative therapy/chemotherapy as well as the relevant reports presented in the ASCO Annual Meeting in 2007 and 2008 were analyzed.The present status of NAC for advanced gastric cancer was summarized,the necessity and feasibility were evaluated,and the patients features for selecting,the predictors for response,the mainly existing problems and development trend of NAC were analyzed.Results At present,there were 7 randomized control trails(RCT) published,and among them 3 were phase Ⅲ.It was safe,effective and feasible to most of trails in NAC for gastric cancer.However,it was still little to obtain survival benefit for NAC RCT,and short of randomized trial comparing strict preoperative chemotherapy to surgery alone or perioperative chemotherapy to surgery plus adjuvant chemotherapy.It remained lots of problems such as how to select the appropriate patients,the effective induced regimes and the predicted factors,the evaluated indices for response.Conclusion NAC is a safe,feasible and efficient method to potentially resectable gastric cancer,but strict phase Ⅲ randomized trials are needed.In the future,substantial improvements of treatment outcome will likely depend on the novel drugs and molecular biological targeted therapies.
4.C/EBP? is involved in transcriptional regulation of mVGLUT2 gene expression
Zhuguo WU ; Shiming LIU ; Xinchun CHEN
Chinese Journal of Pathophysiology 1986;0(01):-
AIM:The main purpose of this study is to investigate the regulatory role of C/EBP? in mouse vesicular glutamate transporter 2(mVGLUT2) gene expression. METHODS:The promoter region of mVGLUT2 was cloned to PGL3-basic vector. Site-direction mutation was used to identify the CCAAT-enhancer-binding protein ?(C/EBP?) binding site. The promoter activity was observed by luciferase system. The binding between C/EBP? protein and mVGLTU2 promoter region was determined by EMSA. The C/EBP? gene expression was inhibited by its specific siRNA. RESULTS:mVGLUT2 promoter activity decreased about 50% after mutation of C/EBP? binding site. EMSA showed that C/EBP? protein bound onto mVGLUT2 promoter region. Meanwhile,when C/EBP? gene expression was inhibited by its specific siRNA,mVGLUT2 promoter activity,mRNA level and protein level were decreased about 60%,40% and 45%,respectively. CONCLUSION:C/EBP? is involved in the regulation of mVGLUT2 gene expression.
5.Study on the Antihepatocarcinoma Activity and the Acute Toxicity of Aclacimomycin-A Solid Lipids Nanoparticle for Injection in Mice in Vivo
Shiming SUN ; Lin HE ; Zhengzhong WU
China Pharmacy 2005;0(18):-
OBJECTIVE:To study the antihepatocarcinoma activity and toxicity of Aclacimomycin-A solid lipids nanoparticle for injection(ACM-SLN)in nude mice in vivo with ACM for injection as the reference preparation.METHODS:The nude mice were divided into control group,ACM group and ACM-SLN group after tumor transplantation,which were injected with the corresponding medicine before the tumor-inhibition rates of which were calculated,which were then injected with ACM and ACM-SLN,respectively before the LD50 of mice were calculated.RESULTS:Compared with the control group,the tumor inhibition rates of ACM-SLN group and ACM group were 78.4%and 38.8%,respectively,the LD50 of which were 16.3 mg/kg and 18.0mg/kg,respectively.CONCLUSION:The ACM-SLN is superior to ACM in terms of the anti-tumor effect while without the increase of toxicity.
6.Clinical Efficacy of Prostaglandin E_1 Used Early for Patients after Renal Transplantation
Hang LIU ; Shiming LIANG ; Xiaohou WU
China Pharmacy 2007;0(35):-
OBJECTIVE:To observe the clinical efficacy of prostaglandin E1 used early in patients after renal transplantation. METHODS:93 renal transplant recipients (treatment group) were treated with PGE1(20 ?g?d-1) within 2 weeks after operation,and another 85 renal transplant recipients (control group) were not given PGE1 therapy in the same period. Urine volume,serum creatinine (Scr),blood flow resistance-indexes (RI) under Doppler color ultrasound,incidences of acute rejection (AR) and delayed graft function (DGF) and the 1 year patient/graft survival rates were compared between the two groups. RESULTS:The 24h urine volume in the treatment group on day 1 after operation was significantly higher than in the control group (9.40?1.9 L vs. 8.11?1.8L) (P
7.C/EBPα is involved in transcriptional regulation of mVGLUT2 gene expression
Zhuguo WU ; Shiming LIU ; Xinchun CHEN
Chinese Journal of Pathophysiology 2010;26(1):142-145
AIM: The main purpose of this study is to investigate the regulatory role of C/EBPα in mouse vesicular glutamate transporter 2(mVGLUT2) gene expression. METHODS: The promoter region of mVGLUT2 was cloned to PGL3-basic vector. Site-direction mutation was used to identify the CCAAT-enhancer-binding protein α(C/EBPα) binding site. The promoter activity was observed by luciferase system. The binding between C/EBPα protein and mVGLTU2 promoter region was determined by EMSA. The C/EBPα gene expression was inhibited by its specific siRNA. RESULTS: mVGLUT2 promoter activity decreased about 50% after mutation of C/EBPα binding site. EMSA showed that C/EBPα protein bound onto mVGLUT2 promoter region. Meanwhile, when C/EBPα gene expression was inhibited by its specific siRNA, mVGLUT2 promoter activity, mRNA level and protein level were decreased about 60%, 40% and 45%, respectively. CONCLUSION: C/EBPα is involved in the regulation of mVGLUT2 gene expression.
8.Content Determination of Costunolide in Liqifuwei Oral Liquid by RP-HPLC
Rongsheng TONG ; Shiming SUN ; Zhengzhong WU
China Pharmacy 1991;0(03):-
OBJECTIVE:To establish a RP-HPLC method for content determination of costunolide in Liqifuwei oral liquid.METHODS:The assay was performed on a Luna C 18 column by UV detector at the wavelength of225nm with methanol-water(72∶28)as the mobile phase at the flow rate of1.0ml/min.RESULTS:The amount of costunolide was linear with its area over the range from0.2?g~2.0?g(r=0.9996),the average recovery was98.4%(RSD=0.85%).CONCLUSION:The present method is convenient,sensitive and accurate with good reproducibility and can be used for the quality control of Liqifuwei oral liquid.
9.TNFAIP8 gene silencing inhibits the migration of mouse RAW264. 7 macrophages
Fei YANG ; Suxia WU ; Shiming FENG ; Guangchao LIU ; Lihui CHAI
Chinese Journal of Microbiology and Immunology 2016;36(4):288-293
Objective To construct a shRNA lentiviral vector targeting the gene encoding tumor necrosis factor alpha-induced protein 8 (TNFAIP8) in RAW264. 7 cells, a mouse macrophage cell line, and to investigate the effects of TNFAIP8 gene silencing on the functions of mouse macrophages. Methods The shRNA sequence targeting TNFAIP8 gene was designed and DNA oligos containing small hairpin frame was synthesized. The double-stranded DNA was cloned into pLKO. 1-TRC vector after annealing. The recombi-nant vector was verified by using double enzyme digestion and gene sequencing. Lentiviruses were prepared by transfecting the constructed vector into 293T cells. Fluorescent quantitative RT-PCR and Western blot as-say were performed to detect the expression of TNFAIP8 at mRNA and protein levels after infecting the RAW264. 7 cells with lentiviruses. Flat dish adhesion experiment and wound-healing assay were used to evaluate the effects of TNFAIP8 gene silencing on the adhesion and migration of RAW264. 7 cells. Results The recombinant lentiviral vector was successfully constructed as indicated by double enzyme di-gestion and gene sequencing analysis. The expression of TNFAIP8 in RAW264. 7 cells at both mRNA and protein levels were significantly down-regulated after lentivirus infection (P<0. 05). Moreover, TNFAIP8 gene silencing significantly impaired the cell adhesion ability of RAW264. 7 cells after 15 min, 30 min or 2 hours of culture. Compared with the cells in control group, the RAW264. 7 cells harboring silenced TN-FAIP8 gene looked round with a smaller number of cellular extensions. The wound-healing assay showed that less TNFAIP8 gene-silenced RAW264. 7 cells migrated into the wounded area as compared with the cells in control group after 24 hours of culture (P<0. 05). The wound-healing rates of the experimental and control groups were 25% and 50%, respectively. Conclusion The recombinant lentiviral vector containing shRNA targeting the TNFAIP8 gene was successfully constructed. Transfecting the RAW264. 7 cells with the con-structed vector significantly silenced the expression of TNFAIP8 gene and inhibited the adhesion and migra-tion of these cells.
10.Determination of Two Ingredients in Calamine and Menthol Lotion by GC-MS
Zhengzhong WU ; Jiying YU ; Lin HE ; Shiming SUN
China Pharmacy 2001;0(12):-
OBJECTIVE:To establish a GC-MS assay for determination of the contents of phenol and menthol in calamine and menthol lotion.METHODS:Using DM-5elastic quartz capillary as separation column,I-octanol as internal standard,phenol and menthol were detected separately under the70℃~150℃step-up hyperthermic condition to select the ion fragment peaks with M/Z of94and71.RESULTS:Phenol and menthol were good linear within1.092~21.840?g/ml(r=0.9998)and1.194~9.552?g/ml(r=0.9999).Recoveries were100.2%(RSD=1.34%)and100.4%(RSD=0.74%)respective?ly.CONCLUSION:The method is quick and accurate with highly repeatability and specificity and it is adequate for contents determination and quality control for this preparation.