Humans ; Mining ; Oxygen ; administration & dosage ; physiology ; Tibet

Objective　To determine the value of spiral CT in diagnosis of endobronchial tuberculosis(EBTB) after analysing the spiral CT appearance. Methods　Retrospective analysis of CT signs in 13 cases who were finally pathologically found suffering from EBTB via biopsy with bronchoscope. Results　Five cases have signs of bronchial structure with obstructive atelectasis and pneumonia. Two cases have signs of irregular bronchial wall. Four cases have signs of scattering dot and patchy increase density in lung fields. Two cases' bronchi and lung tissues are normal. Conclusion　CT examination can determine the site and extent of EBTB and help the localization of bronchoscope.

Clinical teaching supervision system exhibited several problems in the imple-mentation process such as the team of supervision being not reasonable enough and the connotation of supervision not extensive and not going far in depth enough and so on. Through the way of strengthening the team of the clinical teaching construction,expanding the connotation and improving the functions of supervision,we can construct a new effective supervision system for clinical school teaching.

Objective To study the relationship between angiotensin Ⅱ type 1 receptor (AT_~1 R) gene polymorphism and carotid atherosclerosis and pathogenesis in primary hypertension . Methods Polymerase chain reaction combined with restriction enzyme digestion and ultrasonography were used to measure AT_~1 R gene polymorphism and carotid artery intima-media thickness (IMT) separately in 150 patients with primary hypertension compared with 100 controls . Results The frequencies of AC genotype and C allele of AT_~1 R gene in patients were higher than those of control ~(P

That there are so many difficulties in both of teaching and learning electrocar-diogram(ECG)is the characteristics in teaching and studying ECG. The following ways are the ef-fective methods of improving effects of classroom teaching of ECG:emphasizing the importance of ECG in clinical work at the beginning of the lecture on ECG in order that the students can understand the necessity of studying ECG and make up their mind to study ECG well;studying the teaching contents on ECG seriously and supplement the textbook appropriately;set up the link of discussion and create the condition that teacher and students work altogether;produce the slides with pictures(mainly)and words and give accounts of the pictures mainly;sum up the character-istics of all sorts of abnormal ECG and state the characteristics of presenting ECG simultaneously.

Objective To investigate the effect of the treatment of humeral lateral condyle fracture in children through elbow arthroscopy. Method From July 2003 to May 2008, 128 children with humeral lateral condyle fracture were randomly divided into open reduction group and arthroscopy group, each group of 64 cases. Results All 128 children received 12-24 months of follow up, with average of (18.07 ± 5.63) months. In arthroscopy group, the blood loss averaged(34.6 ± 9.1) ml, incision length of(0.98 ± 0.20) cm, and after the first 3 days the VAS was(3.99 ± 1.33) scores. However, in open reduction group, the blood loss averaged (109.9 ± 18.9) ml, incision length of (5.38 ± 1.30) cm, and the VAS was (7.03 ± 2.80) scores. All those in arthroscopy group were much better than those in open reduction group,the differences were statistically significant (P<0.01 or < 0.05). According to Mayo score, the total fine rate was 85.9%(55/64) in open reduction group, and 98.4%(63/64) in arthroscopy group (P<0.05). About in the incidence of postoperative complications,there were 8 cases of delayed healing, S cases of inside inversion or outside eversion elbow varus deformity, 9 cases of pin tract infection in open reduction group. However there were 1 cage of delayed healing, 2 cases of inside inversion and outside eversion elbow varus deformity, 1 case of pin tract infection in arthroscopy group(P< 0.01). Conclusion Arthroscopy operation has the advantages of the small incision, reset reduction accurate, less complications ,and is an effective method of treating the lateral condyle fractures in children.

Objective To build a scientific,rational and practical evaluation index system for medical tutors.Methods Evaluation framework was initially constructed on the basis of literature analysis.Two rounds questionnaires investigation were conducted soon using Delphi method and the data were analyzed using descriptive analysis,independent samples of non-parametric test (Kreskas-Wallis test),expert reliability analysis,analytic hierarchy process and other methods.Results The results of two rounds of expert questionnaires showed that both recovery rate and acceptance rate of the questionnaires were more than 90％ and overall expert coefficient was 0.8957.The coordinate coefficient of the second-class index was 0.181,x2 =27.148,P ＜ 0.001,and that of the third-class index was 0.157,x2 =127.03,P ＜ 0.001.Experts' opinions were highly consistent.Conclusions Evaluation index system including three first-class indexes,seven second-class indexes and twenty-eight third-class indexes was established for medical tutors.The evaluation index system is of great importance in safeguarding postgraduate culturing quality and strengthening teaching staff construction.

AIM: The main purpose of this study is to investigate the regulatory role of C/EBPα in mouse vesicular glutamate transporter 2(mVGLUT2) gene expression. METHODS: The promoter region of mVGLUT2 was cloned to PGL3-basic vector. Site-direction mutation was used to identify the CCAAT-enhancer-binding protein α(C/EBPα) binding site. The promoter activity was observed by luciferase system. The binding between C/EBPα protein and mVGLTU2 promoter region was determined by EMSA. The C/EBPα gene expression was inhibited by its specific siRNA. RESULTS: mVGLUT2 promoter activity decreased about 50% after mutation of C/EBPα binding site. EMSA showed that C/EBPα protein bound onto mVGLUT2 promoter region. Meanwhile, when C/EBPα gene expression was inhibited by its specific siRNA, mVGLUT2 promoter activity, mRNA level and protein level were decreased about 60%, 40% and 45%, respectively. CONCLUSION: C/EBPα is involved in the regulation of mVGLUT2 gene expression.

Objective To observe the changes of behaviour, NOS neurons, Nissl bodies and ultrastructure after L-NNA treatment of acute spinal cord injury in rats. Methods Using movement and incline plane(IP)score to observe hindlimbs movement of rats with spinal cord injury; NADPHd histochemistry, Nissl methed and electron microscopy were used for observation of changes of neuronal NOS, Nissl bodies and ultrastructure of three groups(normal control group, saline solution(NS)control group and L-NNA group). Re- sults 1. Changes of behaviour: Hindlimbs movement and IP score in L-NNA group is more potent than that in NS group(P

Objective To investigate and analyze the state of injury and treatment for the patients with maxillofacial injuries in Wenchuan earthquake, and search for more effective methods for treating maxillofacial injury. Methods One hundred and sixty-five patients wounded in Wenchuan earthquake were admitted to authors' hospital, and they received specialized diagnosis and treatment. In this paper, the emphasis was put on those patients with severe ear-nose-throat and maxillofacial injuries. Based on the analysis of the state of injuries, the patients were treated with debridement and suture, reshaping and suture, reduction and fixation of fracture, or local or systematic anti-infection therapy. All the treated patients were followed up 2 weeks after treatment. Results Thirty-three of the 165 admitted patients suffered from various degrees of maxillofacial injury (20.0%), including 10 cases of ear injuries (6.1%), 8 cases of nasal trauma (4.8%) and 2 cases of lip laceration (1.2%). All the wounds healed by primary intention after the treatment with no disfiguration. 8 cases of nasal sinus and zygomatic fractures (4.8%) and 5 cases of mandibular fracture (3.0%) recovered nicely after the treatment. No trauma was found in throat, neck, trachea and esophagus. 6 patients (3.6%) were complicated with infection after injury (onset of infection outside of hospital). There was no death. Conclusion Patients with maxillofacial injury should receive early and appropriate treatment. Besides restoration of function, it is also important to restore the physiognomy