ObjectiveTo study the chemical constituents of Citrullus colocynthis.MethodsThe chemical constituents were isolated and purified by column chromatography on silica gel and Sephadex LH-20 and recrystallization as well.NMR spectra and physicochemical property were characterized for structural identification.ResultsEleven compounds wereisolatedandidentifiedasβ-sitosterol(1), α-spinasterol-3-O-β-D-glucopyranoside(2),α-spinasterone (3),bis (2-ethylhexyl) phthalate (4),p-hydroxybenzoic acid (5),6-C-p-methylbenzoylvitexin (6),dihydrocucurbitacin E (7),cucurbitacin E (8),dihydro-epi-iso-cucurbitacin D (9),dihydroisocucurbitacin B-25-acaetate (10),and cucurbitacin E 2-O-β-D-glucopyranoside (11).ConclusionCompound 6 is a novel compound.Compounds 1-5,7,9,and 10 are isolated from C.colocynthis for the first time.

OBJECTIVE To compare the clinical efficacy between adenoidectomy combined with auripuncture (AT+A) and adenoidectomy combined with tympanostomy tubes (AT+T) in children wiht otitis media with effusion. METHODS 92 cases of children with otitis media with effusion were selected from September 2010 to September 2014 in our hospital which was divided into AT+T group and AT+A group, depending on therapy methods.There were 48 in AT+T group and 44 in AT+A group. Retrospective analysis were made on a series of clinical data ,including the therapeutic effect, hearing threshold before and after treatment, infection rate and recurrence rate. RESULTS The hearing threshold has been significantly reduced within one year of follow-up after treatment,however,there was no significant difference (P>0.05). Total efficiency rate was 97.9% in AT+T group and 95.4% in AT+A group, no significant difference was found (P>0.05). The average middle ear fluid duration, infection and recurrence rate of children in AT+T group and AT+A group was (7.3±0.8) days, 6.3%, 4.2% and (11.7±0.4) days, 15.9%, 11.4% respectively, there was significant differences (P<0.05). CONCLUSION Both AT+T and AT +A have a good therapeutic effect for children with otitis media with effusion, which can significantly improve the hearing. Compared to AT+A, AT+T have a higher recovery rate,significantly reduce the recurrence and infection rate,and may be more suitable for clinical treatment of children with otitis media with effusion.

Object To study the chemical constituents of our folk herb, Cucubalus baccifer L Methods The components were separated on Diaion HP 20 and silica gel column chromatography and the structures were identified by spectral evidence Results Six compounds from ethyl acetate extracts were elucidated as 6? methoxy piperidin 2 one (Ⅰ), pterolactam (Ⅱ), 5, 7, 4′ trihydroxyflavone (Ⅲ), 4 hydroxy 3 methoxybenzopropanyl acid (Ⅳ), 4 hydroxybenzoaldehyde (Ⅴ), and 4 hydroxybenzoic acid (Ⅵ) Conclusion Compound Ⅰ was a new naturally occurring compound and others were first isolated from this plant

Background Corneal refractive surgery has significant effects on corneal biomechanical properties.However,there are few study on large scale population with corneal biomechanical properties in the myopic population before corneal refractive surgery.Objective This study was to evaluate the change of corneal biomechanical properties and influence factors in myopic eyes using ocular response analyzer (ORA).Methods A prospective cohort study was carried out in Beijing Tongren Eye Center from April 2010 to January 2011.Corneal hysteresis (CH) and corneal resistance factor (CRF) were detected using ORA in 1 792 eyes of 896 myopic subjects who were going to receive corneal refractive surgery under the informed consent.According to different spherical equivalent (SE) the eyes were grouped into-0.25-＜-3.0 D group,-3.0-＜-6.0 D group,-6.0-＜-9.0 D group,-9.0-＜-12.0 D group,-12.0-＜-15.0 D group and ≥-15.0 D group and according to different central corneal thickness (CCT),the eyes were grouped into ＜500 μm group,500-＜550 μm group,550-＜600 μm group and≥600 μm group.The CH value and CRF value in different SE groups or different CCT groups were compared,and the factors influencing CH and CRF were analyzed by Pearson correlation analysis.Results The mean CH and CRF value were (9.84±1.52)mmHg (1 mmHg =0.133 kPa) and (10.46±1.71) mmHg,respectively in the myopic eyes.The CH values were (10.35±1.53),(10.07±1.55),(9.81±1.46),(9.71±1.59),(9.35±1.55) and (9.23±1.28) mmHg in the-0.25-＜-3.0 D group,-3.0-＜-6.0 D group,-6.0-＜-9.0 D group,-9.0-＜-12.0 D group,-12.0-＜-15.0 D group and ≥-15.0 D group,respectively,showing a significant difference among the groups (F=20.69,P＜0.01),and the CH values in the-6.0-＜-9.0 D group,-9.0-＜-12.0 D group,-12.0-＜-15.0 D group and ≥-15.0 D group were significantly lower than those in the-6.0-＜-9.0 D group,-9.0-＜-12.0 D group (all at P＜0.01).There was no significant difference in the CRF values among different SE groups (F =0.65,P =0.49).CH and CRF values were increasing with the raise of CCT,with evidently differences among the CCT＜500 μm group,500 μm ≤ CCT＜550 μm group,550 μm≤ CCT＜600 μm group and CCT≥ 600 μm group (CH:F=110.99,P＜0.01;CRF:F =84.35,P＜0.01),and the CH and CRF values were significantly higher in the 550 μm ≤ CCT＜600 μm group and CCT ≥600 μm group than those in the CCT＜500 μm group (all at P＜0.01).CH showed a positive correlation with SE (r =0.24,P＜0.01),and no significant correlation between CRF and SE (r =0.03,P =0.20).The CH and CRF showed the positive correlations with the CCT (r=0.42,0.57,both at P＜0.01).In addition,CH and CRF values increased as the increases of SE,corneal curvature (CC) and CCT values,with the regression equation of CH =-7.87+0.08SE+0.16CC+0.02CCT (R2=0.26,P＜0.01) and CRF=-11.42+0.14CC+0.03CCT (R2=0.34,P＜0.01),respectively.The CH and CRF did not change with the age and gender in the subjects.Conclusions Corneal biomechanical properties including CH and CRF were positively correlated with CCT,CC and SE.

Asthma ; diagnosis ; Diagnostic Errors ; Humans ; Neurilemmoma ; diagnosis

OBJECTIVE: To construct and obtain ideal protein delivery vectors by researching the delivery efficiency and cytotoxicity to Hela cells using mPEG-PLGA-BSA-FITC-NPs. METHOD: The mPEG-PLGA nanoparticle was obtained through surface modification of PLGA with PEG, and deliver BSA-FITC into Hela cells in vitro. The positive cells were counted by Laser scanning confocal microscopy and the survival rate of Hela cells was calculated by MTT assay at different time points. RESULT: mPEG-PLGA-BSA-FITC-NPs shows the classic nanometer size, and the encapsulation efficiency reached 51. 2%. At the same time, the nanoparticles possess characteristics of slow release. By optimizing the delivery conditions, the highest efficiency of mPEG-PLGA-BSA-FITC-NPs was above 65.2%, and the cellular viability was about 85.7%. CONCLUSION mPEG-PLGA-BSA-FITC-NPs nanoparticles can successfully carry the target protein into cells as safe and effective as novel delivery materials of protein in vitro, and has shown slow release characteristics. The mPEG-PLGA-BSA-FITC-NPs provide ideal delivery vector for future application in clinical treatment of disease using nano-materials.
Drug Carriers ; Fluorescein-5-isothiocyanate ; analogs & derivatives ; HeLa Cells ; Humans ; Nanoparticles ; Particle Size ; Polyesters ; Polyethylene Glycols ; Serum Albumin, Bovine