Objective To explore the main reason of secondary barrenness resulted from drug miscarriage before childbearing. Methods Gynecologic examination was performed in 156 patients with secondary barrenness after drug miscarriage. Uterus neck and vaginal smear examination, mycoplasma and chlamydozoon detection, and hysterosalpingograghy were performed in the same time. Results 101 patients (101/156,64.74%) had genital duct inflammation,61 patients (61/156,39.1%) had various degrees of tubal obstruction. Conclusion The main reason of secondary barrenness after drug miscarriage was the tubal obstruction resulted from inflammation, especially chlamydozoon and mycoplasma infection. Drug miscarriage was not so safe before childbearing.

Objective To establish a stable implanted model of VX2 rabbit brain tumor, and to evaluate the pathological and imaging features and tumor angiogenesis. Methods Thirty New Zealand white rabbits were implanted with 100 ?l viable VX2 tumor cells (107/ml) through a hole 5 mm to the right of the sagittal suture and 5 mm posterior to the coronal suture bored by a dental drill. MRI was performed every ~2 days after 7 days of implantation to evaluate the growth of the tumor, and perfusion CT studies were performed in different days of tumor growth. After that the animals were sacrificed on days 14, 18, 22, 26, and 30 of tumor implantation. 2% Evans blue (2 ml/kg) was given intravenously in 16 of these animals ~1 hour prior to sacrifice to detect the breakdown of the blood-brain barrier (BBB). The specimens of the rabbit brains were examined pathologically and histologically. VEGF and MVD were evaluated in immunohistochemical examination. Results Of the 22 animals included into the study, the tumor grew in 20 animals, which could be seen clearly on MR imaging. Pathologic examination showed characteristics of squamous carcinoma. VEGF was expressed in all tumors with the mean rate of positive cells of (52.51?19.15)% (19.5%-92.9%). Mean MVD was (51.30?14.42)pice piece/microscope (25-81 pice piece/microscope). Using Pearson′s linear correlation analysis, positive correlation was found between tumor growth time and volume (r=0.791, P=0.000), between MVD and tumor growth time (r=0.875, P=0.000), and between MVD and tumor volume (r=0.901, P=0.000), respectively. Spearman′s rank correlation analysis showed positive correlation between VEGF grade and blue stain of the tumor (r_s=0.594, P=0.015). Conclusion A stable model of VX2 rabbit brain tumor has been established with the method of skull drilling. The method was simple and easy to use, with a high tumor growth rate and remarkable angiogenesis. The model is helpful for the pathological and radiological study of tumor angiogenesis.

Objective To study the complications and the outcome of different type of twin pregnancy. Methods Three hundred and thirty two cases of twins from 1981 to 2000 were analyzed retrospectively. Results The incidence of twins is 8.63%.The common complications were pregnancy induced hypertension, premature rupture of fetal membranes, anaemia, premature labor and postpartum hemorrhage. There were no differences between the monozygotic twins and dizygotic twins in the incidences of these complications. The incidence of one fetal death in monozygotic twins was higher than that of the dizygotic twins (P

AIM To research the therapeutic effects of Hanbi Formula (Astragali Radix,Aconiti Radix cocta,Scorpio,Scolopendrap and Pheretima) on adjuvant-induced arthritis rats (RA) and its mechanism of action.METHODS RA rat models were established by using Freund's adjuvant,and then the rats were divided into six groups,namely control group,model group,dexamethasone positive group,Baoguang Fengshi Liquid (Notopterygii Rhizoma et Radix,Radix angelicae pubescentis,Chuanxiong Rhizoma,etc.) positive group,and low,high doses of Hanbi Formula groups.The volume and swelling of toes were measured.The interleukin-1 β (IL-1β) and tumor necrosis factor-α (TNF-α) of serum were detected by ELISA;the proliferative capacity of lymphocytes was tested by methyl thiazolyl tetrazolium (MTI) method;synovial tissue was histopathologically examined with HE staining.Finally,the expressions of interleukin-17 (IL-17) and TNF-α in synovial tissue were determined by immunohistochemical assays.RESULTS Hanbi Formula could significantly relieve toe swelling of RA rats.Compared with the model group,Hanbi Formula could significantly alleviate synovitis in rats with RA,down-regulate the expressins of IL-1 β and TNF-α in serum and synovial tissue,and inhibit lymphocyte proliferation.There were no significant differences in above indices between low-dose and high-dose Hanbi Formula groups,which was quite with Baoguang Fengshi Liquid,but less than dexamethasone.CONCLUSION Hanbi Formula possesses an obvious function of anti-RA,and its mechanism may be related to the inhibition of lymphocyte proliferation and reducing secretion of inflammatory cytokines.

Capital Medical University Pediatrics Online Course which has been set since June 2013 provides students with more personalized and efficient learning platform in line with modern educational concepts.The paper has introduced the application platform,basic framework and usage of the Online Course,analyzing the characteristics of excellent teaching resources,bilingual teaching,students' participation and students' learning more in line with manifestations habits,and finally reflected on the subsequent development of Pediatrics Online Course in the future.

Objective To investigate the changes of the neuron-specific enolase(NSE) and soluble intercellar adhesion molecule-1(CAM-1) in serum and cerebrospinal fluid(CSF) in the patients with viral encephhalites (VE). Methods The levels of NSE and sICAM-1 in serum and CSF were determined before and after treatment using ELISA in 58 patients with VE and 20 normal persons. Results The levels of NSE and sICAM-1 in serum before treatment were obviously higher than those of control group, and their differences were significant (P0.05). Conclusions NSE and sICAM-1 may contribute to pathologic course in infection of VE and the levels of NSE and sICAM-1 in serum and CSF may serve as markers of differential diagnosis and clinical significance.

Objective:To observe the effect of metformin on the expression of SIRT1 and UCP2 in rat liver of type 2 diabetes mellitus (T2DM) with nonalcoholic fatty liver disease (NAFLD), and discuss the pathogenesis of T2DM with NAFLD, and the treatment with and possible mechanism of metformin.Methods:Thirty-six male SD rats were randomly divided into a normal control group (group NC, n=12), a T2DM with NAFLD group (group MC, n=12), and a metformin group (group A, n=12). We established the model of T2DM with NAFLD rats by feeding high-fat and high-sugar diet and injecting STZ. After the success establishment of the model, the metformin group was given metformin 300 mg/(kg.d) for 8 weeks. At the end of the experiment, we measured FBG, ALT, AST, TC, TG, HDL-C, LDL-C, VLDL, FFAs, FINs and HOMA-IR respectively in group NC, MC and A. We observed the change of liver tissue pathology by HE, determined the expression of SIRT1 and UCP2 in rat liver by immunohistochemical method and real-time quantitative method. Results:FBG, ALT, AST, TC, TG, LDL-C, VLDL, FFAs, FINs and HOMA-IR were higher in group MC than in group NC (P<0.05), while HDL-C was obviously lower in group MC than in group NC (P<0.05). After the metformin treatment, the serum parameters in the rats had improved in group NC compared with in group MC (P<0.05). On immunohistochemical staining and mRNA level, the expression of SIRT1 was obviously lower in group MC than in group NC (P<0.05), and the expression of UCP2 was obviously higher in group MC than in group NC (P<0.05). After the metformin treatment, the expression of SIRT1 was higher than in group MC (P<0.05), and the expression of UCP2 was lower than in group MC (P<0.05). There was negative correlation between the expression of SIRT1 and UCP2 (r=-0.61, P<0.01).
Conclusion:The expression of SIRT1 is low and the expression of UCP2 is high in rat liver of T2DM with NAFLD. Metformin can increase the expression of SIRT1 and reduce the expression of UCP2, with negative correlation between the expression of SIRT1 and UCP2.

Objective To explore β-sodium aescinate on vascular endothelial function ( FMD ) , homocysteine ( Hcy ) and hypersensitive C-reactive protein ( hs-CRP) and clinical efficacy in patients with acute cerebral infarction.Methods 198 acute cerebral infarction patients from March 2013 to April 2015 were randomly divided into observation group (n=100) and control group (n=98).Control group were treated according to the condition of the disease, observation group were treated by β-sodium aescinate base on control group, 20mg was added to 250mL saline for intravenous drip,one times per day.Continuous used 14d for one treatment courses.Compared the change of vascular endothelial function, Hcy and hs-CRP and clinical efficacy.Results The total effective rate of observation group was 90.00%, which was significantly higher than that of 71.42% in control group (χ2 =11.01,P<0.05).Post-treatment the value of FMD significantly increased, Hcy and hs CRP were significantly decreased both in observation group and control group respectively, which the difference had a statistically significant as compared with Pre-treatment (P<0.05);but, the value of FMD was significantly higher, Hcy and hs CRP was significantly lower in observation group than that of control group (P<0.05).Conclusion It has a significant β-sodium aescinate clinical effect in treatment of acute cerebral infarction, and FMD are significantly higher, Hcy and hs-CRP are significantly decrease.

Objective To evaluate the influence of different diagnostic criteria of gestational diabetes mellitus(GDM) using oral glucose tolerance test(OGTT) on pregnant outcome. Methods A retrospective study of women who delivered at Peking University First Hospital from Jan 1, 2001 to Dec 31,2003 was conducted. All women with abnormal 50 g oral glucose screening test received 75 g OGTT. Totally, there were 850 cases not being diagnosed as GDM or GIGT according to Fernando′s criteria and these women were divided to two groups according to Zhi guang DONG′s criteria: group Ⅰ included those who meet the criteria of GDM and GIGT and group Ⅱ included those who did not. Results The rate of abnormal OGTT was 23.76% (GDM 4.35%, GIGT 19.41,37 vs 165 cases). The average birth weight of group Ⅰ was significantly higher than that of group Ⅱ [(3461.4?475.9)g vs (3407.8?438.4) g, P

Immune thrombocytopenia (ITP) is a kind of acquired autoimmune disease with complex and diverse mechanism.However,at the moment,the pathogenesis is still not very clear.It is confirmed that the pathogenesis of ITP is mainly related to humoral immunity and cellular immunity.It is generally believed that genes and environmental factors are of great significance to the development and progress of ITP.In the genetic background of susceptible genes,the development of ITP is due to environmental factors induced by autoimmune response.More genes are found:protein tyrosine phosphatase nonreceptor 22 (PTPN22) gene single nucleotide polymorphisms,micro RNA (Micro RNA),human leukocyte antigen-HLA -DRB1 gene polymorphism,DNA methylation,vanin-1 gene.This article reviews the genes involved in the pathogenesis of ITP.