2.The protective effects of quercetin et al on the intestinal mucosal injury induced by burn in mice
Desheng MENG ; Shiliang WANG ;
Chinese Pharmacological Bulletin 1986;0(06):-
AIM To investigate the protective effects of three flavonoids quercetin,rutoside and flavone on the intestinal mucosal injury induced by burn in mice. METHOD The change of injury index of small intestine was assayed and the content of protein and DNA were measured. DTNB method were adopted to measure the GSH content in the intestinal mucosa. The level of DNA damaged were determined by measuring the fluorescence. RESULTS The condition of injury of intestinal mucosa were improved in varying degrees after these favonoids were given introgastrically for 3 days. The injury index of intestinal mucosa decreased and the content of protein,DNA and GSH increased. The degrees of DNA damage were reduced also.Quercetin was the most effective one among these three flavonoids. CONCLUSION The flavonoids of quercetin, rutoside and flavone have obvious protective effects on burn induced intestinal mucosal injury, and the mechanism may be related to its antioxydation function.
3.Effects of early enteral feeding containing medium and long-chain triglyceride on improving protein catabolism of burned patients
Parenteral & Enteral Nutrition 1997;0(02):-
Obfectives:The purpose of the present study was to compare the effects of different early enteral nutrition formula (containing either MCT/LCT or LCT only) on improving nutritional status of burned patients. Methods:This was a prospective,randomized,single blind and controlled study.Thirty cases of burned patients injured by varous causes with total burn surface area (TBSA) more than 30% were collected and randomized into two groups:①Group F:enteral feeding with Fresubin 750 MCT containing MCT/LCT as fat source,②Group N:enteral feeding with Nutrison,containing only LCT as fat source.All of the thirty patients received isocaloric,isonitrogenous,and isolipidic enteral feeding,with intragastric tube and enteral feeding pump,started within 24 h postburn.The several parameters were observed on postburn day(PBD) 1,4,7 and 10,such as serum concentrations of transferrin and prealbumin and plasma concentrations of prostaglandin E 2(PGE 2) and insulin.Nitrogen in urine per day and excretion of 3 methylhistidine in urine on day 1,7 and 10 postburn were determined. Results:The results showed that the patients of F group had an increased blood concentration of transferrin,prealbumin and insulin,and a decreased plasma concentration of PGE 2.Excretion of nitrogen and 3 methylhistidine in urine was also markedly reduced in patients fed with nutrients containing MCT/LCT compared with LCT. Conclusions :We have shown that an enteral diet containing MCT/LCT can improve the nutrition status of burned patients more effectively.
4.Construction,expression and purification of pTAT-HIF-1?-PAS-B in prokaryotic expression system
Huabing QI ; Shiliang WANG ; Fengjun WANG
Journal of Third Military Medical University 2003;0(22):-
Objective To construct a prokaryotic expression vector for a fusion protein, TAT protein transduction domain (PTD) and the PAS-B domain of hypoxia inducible factor 1?(HIF-1?), and then express and purifr the fusion protein. Methods The expression plasmids pTAT-PAS-B, pET-PAS-B and pTAT-EGFP were constructed respectively, and transformed into E. coli. BL21(DE3)pLysS strain to be induced by IPTG. The obtained proteins were analyzed by SDS-PAGE and Western blotting. The fusion protein were purified with Ni-NTA-His affinity chromatography. Results The three recombinant plasmids were constructed successfully. The objective fusion proteins were obtained by optimizing the conditions for expression and purification. Conclusion The successful expression and purification of the fusion protein TAT-HIF-1?PAS-B has laid the foundation for using it to modulate the activity of HIF-1? in vivo.
5.Effects of quercetin on metabolism of cyclooxygenase in IEC-6 cells stimulated by the serum of burned rats
Desheng MENG ; Fengjun WANG ; Shiliang WANG ;
Journal of Third Military Medical University 2003;0(15):-
Objective To observe the effects of quercetin on PGE 2 release and cyclooxygenase activity in IEC 6 cells stimulated by the serum of burned rats. Methods The PGE 2 levels were measured by radioimmunoassay and the changes of cyclooxygenase activity were determined by substrate fluorescence analysis. Results Stimulated by the serum of burned rats, the PGE 2 level increased first and then obviously decreased. Quercetin at 0.1 and 1.0 ?mol/L enhanced the PGE 2 level after the stimulation of IEC 6 cells for 24 h. There was no obvious change in total cyclooxygenase activity, but the activity ratio of COX 1 to COX 2 significantly decreased. Conclusion Quercetin might influence the release of cyclooxygenase metabolite PGE 2 through the regulation of COXs activity in IEC 6 cells.
6.An experimental study of glucose transporter 1 protein expression in the burned rat liver
Huabing QI ; Fengjun WANG ; Shiliang WANG ;
Journal of Third Military Medical University 2003;0(18):-
Objective To investigate the expression of glucose transporter 1(GLUT 1) and its transcription activity in the liver of burned rats Methods The Wistar rats inflicted with 30% TBSA full thickness flame burn on the back were sacrificed at 0 5, 1, 2, 4, 8 and 16 h after burn GLUT 1 protein levels in the rat livers were determined by Western Blotting with the reference to those in 6 normal rats The liver cells were transfected with Construct A, and 24 h later subjected to hypoxia (1%O 2) to mimic the hypoxia environment The samples were harvested at 3, 6 and 12 h and determination of the reporter gene luciferase and pSV ? galactosidase activities was performed Results ①Compared with that in the normal control, GLUT 1 protein level in the liver was significantly increased( P
7.Experimental study of mechanisms of hypoxia-induced expression of glucose transporter 1 in rat liver cells
Huabing QI ; Fengjun WANG ; Shiliang WANG
Journal of Third Military Medical University 1983;0(03):-
Objective To explore the mechanisms of hypoxia-induced expression of glucose transporter 1 (GLUT-1) in rat liver cells. Methods Wild type hypoxia response element (HRE) plasmid (construct N) containing the potential HIF-1 binding site was constructed by using construct A which contains the full length of the 5′-flanking region of the rat GLUT-1 gene as a template of PCR, and the PCR product was subcloned into the reporter plasmid pGL3-Promoter. Mutation type HRE plasmid (construct M) was made using a two-step overlapping PCR strategy. Then the liver cells were transfected with constructs N and M, respectively. At 24 h after transfection, the cells were subjected to hypoxia (1% O 2) to mimic the hypoxic environment caused by burn for 12 h. The samples were harvested and the determination of the reporter gene luciferase activity and pSV-?galactosidase activity was performed. Results Constructs N and M were successfully constructed and the liver cells were successfully transfected with construct N or construct M. Hypoxia induced more enhanced luciferase activity of constructs N and M as compared with the control (P
8.Efficacy of Reduced Glutathione Against Hepatic Lesion Induced by Antituberculotic Agents
Aiqin WANG ; Shiliang ZHONG ; Zonghua RAO
China Pharmacy 2001;0(11):-
OBJECTIVE:To observe the efficacy of the reduced glutathione against hepatic lesion induced by antituberculotic agents.METHODS:A total of 226 patients with pulmonary tuberculosis undergoing short- course chemotherapy were randomly assigned to receive antituberculotic agents plus reduced glutathione throughout the course(treatment group,n = 114 cases) or to receive Inosine plus Glucurolactone(control group,n = 112).The patients were followed for 3~6 months with the incidence of hepatic lesion compared between the two groups.RESULTS:There were significant differences between the treatment group and the control group in the incidence of hepatic lesion,6 cases(5.3%) in treatment group vs.29 cases (25.9%) in the control group(P
9.A Clinical Study of Tolbutamide to Improve Hypermetabolisnl in Burn Patients
Weiguang XIE ; Ao LI ; Shiliang WANG
Journal of Third Military Medical University 1983;0(04):-
Relative deficiency of serum insulin and insulin resistance of tissues are the main causes of postburn hypercatabolism.In order to evaluate the clinical effects of tolbutamide to improve postburn hypermetabolism,9 burn patients were given tolbutamide and observed for 10 days after the drug had gone through animal experiments.It was found that tolbutamide could improve postburn hyper-metabolism through following ways:1.To stimulate the secretion of insulin and enhance the effect of insulin.2.To reduce the insulin resistance of tissues and promote glucose utilization of skeletal muscles.3.To inhibit the secretion of glucagon.
10.Preliminary application of the grey system forecast in buru medicine
Daizhi PENG ; Shiliang WANG ; Ruzhou MA
Journal of Third Military Medical University 1988;0(05):-
The GM(1,1)model of the grey system forecast was used to process and analyze the total annual admission of patients,the total number of annual patient death,and the annual mortality rate of the burn patients of the Institute in the 5-year-period from 1986 to 1990.3 corresponding forecast models were established respectively.The forecast values by these models were quite well accordant with the actual values in the period from 1986 to 1990.The forecasts of 1991 by these models are that the total annal admission of patients will approach that of 1989,which was the highest one in the last 5-year-period,and the total number of annual patient deatb and annual mortality rate will be slightly higher than that of 1990,which was the lowest one in the last 5-year-period.The grey system forecast will be beneficial for the work-planning and the personnel arrangement of our Institute.