Objective To study the transplantation of embryonic full-thickness retina, including neural retina and retinal pigment epithelial (RPE), into the subretinal space of rats and to investigate the development of the grafts. Methods A total of 34 eyes from 3-week-old pigmented rabbit embryos were treated with the dispase to obtain pieces of the full-thickness retina, and then grafts were injected into the subretinal space of the right eyes of 20 Wistar rats and 14 RCS (Royal college of surgeons) rats. Host rats were sacrificed at 3, 7, 14, and 28 d after transplantation. Sections were stained with hematoxylin eosin and immunohistochemistry. Results The rabbit embryonic retina was capable of developing and differentiating in the subretinal space of the host rats. Conclusion The rabbit embryonic retina is capable of developing and differentiating in the subretinal space of host rats. Transplantation of xenografts of embryonic full-thickness retina is practicable, but the methods of transplantation need to be improved.

Aim To observe the cytotoxic and proliferous effects of Nitraria tangutorum flavone(NTF)on human A-704 strain cells.Methods In vitro cultured human A-704 strain cells,the cytotoxic and proliferous effects of NTF on human A-704 strain cells were determined with methods of trypan-blue and clone formation,and the microelement in cell was detected with X-electron microscope scanning.Results ① The trypan-blue detection showed that the living cells in the NTF group(0.1,0.5 and 5 mg?L-1)and the 5-FU group were decreased by 33.8%~57.3%,35.8%~80.1%,60.8%~99% and 40.3%~59.6% at 6~18 hour after treatment,which significantly differed from those of the control group(P

Objective To find out the classification of diseases contracted by patients of the emergency department.Methods A retrospective investigation and analysis was made of the medical records of 82 598 emergency cases in our hospital. Results Injury and poisoning, respiratory disease, and disease of digestive system were the first three kinds of disease treated by the emergency department; male emergency cases outnumbered female ones; the volume and visiting hours of emergency cases in a typical day take the shape of humps, with 19 to 21 hours being the climax; the ages of emergency cases averaged 41.5, with patients of the age group running from 19 to 35 being the largest in number in 16 kinds of disease. Conclusion ①It is imperative to determine the priorities of technology construction and the orientation of talent cultivation in the emergency department according to the disease spectrum of emergency cases. ②It is necessary to attach importance to the education of males in health, safely and precautions. ③It is essential to fully recognize the great significance that enhancement of the expertise of the emergency department carries with regard to the health of the population. ④It is important to rationally plan work in accordance with the rush hours of emergency cases's visits.

Objective To explore the clinical value of multi-slice CT enhancement perfusion imaging (MSCTPI) in diagnosis and differential diagnosis of thyroid disease. Methods Thirty-three patients with benign thyroid diseases were enrolled in the benign group, and were divided into subgroups of nodular goiter (n=17) and thyroid adenoma (n=16), while 10 patients with thyroid carcinoma were enrolled in the malignant group. All patients underwent routine CT scanning and MSCTPI with GE LightSpeed 16-detector row CT scanner. Time-density curve (TDC) of common carotid for benign thyroid diseases and thyroid carcinoma was depicted. Perfusion parameters of blood flow (BF), blood volume (BV), mean transit time (MTT) and permeability surface area product (PS) were obtained automatically. All parameters were statistically analyzed among groups. Results TDC showed single peak in common carotid artery, with small peak of speed up and slow down in benign group, while with baseline segment, up above, down segment and horizontal segment in thyroid carcinoma. There was statistical difference between benign and malignant groups in BF, BV, MTT and PS value (P=0.001, <0.001, 0.003 and <0.001, respectively). No significant difference of BV and MTT was found between subunits of benign and thyroid carcinoma (all P>0.05). BF was significantly different in benign and maligant groups (P<0.05), whereas PS in thyroid carcinoma and nodular goiter was significant different (P<0.05). No statistical difference of BF, BV, MTT and PS was detected between nodular goiter and thyroid adenoma. Conclusion MSCTPI can exactly show the blood flow features of thyroid. The analysis of BF, BV, MTT and PS is helpful for differential diagnosis between benign thyroid disease and thyroid carcinoma.

Nerve growth factor (nerve growth factor, NGF) is the earliest discovered growth factor in neurotrophic factors,it has a very important role in maintaining the survival, growth, differentiation,repair and regeneration after injury of neurons. In this paper,research progress in recent years on NGF are reviewed.

Objective To investigate the association of leiomyoma with death receptors(DR) and the mechanisms underlying the development of leiomyoma.Methods The semi-quantitative RT-PCR was employed to examine the mRNA expression of apoptosis-associated death receptors in the Leiomyoma tissue samples collected from 20 clinical cases.Results It was found that the mRNA expression of DR4 in leiomyoma tissue was dramatically decreased as compared to that of normal tissue controls (P<0.001),but no difference was found between leiomyoma and normal tissue controls in terms of DcR1,DcR2,DR5,TNFR1A,TNFR1B and Fas mRNA expressions (P>0.05).Conclusion It is highly possible that DR4 expression might be involved in the development of leiomyoma.More efforts will be required to elucidate the exact mechanisms underlying the development of leiomyoma.

Objective: To establish a mice model of congenital heart disease transposition of great arteries in order to provide a research reference for the occurrence and development of transposition of great arteries. Methods: A total of 20 pregnant ICR mice at 8-10 weeks of age were divided into 2 groups: Control group, the mice received a single dose of DMSO 70 mg/kg at 8.5 days of gestation,n=5 and Experiment group, the mice received a single dose of all-trans retinoic acid 70 mg/kg at 8.5 days of gestation,n=15. All animals were treated for 18 days and then the embryos were taken to observe cardiac morphology under stereomicroscope. Results: Compared with Control group, Experiment group had obviously increased occurrence rates of premature delivery, abortion and embryo absorption, and 61.2% phenotype for transposition of great arteries; meanwhile, combining with non-heart defect phenotypeas exophthalmos and spinal malformation. Conclusion: All-trans retinoic acid may induce transposition of great arteries in mice embryos, which is a feasible animal model in experimental research.

Objective]To evaluate the value of three-dimensional transesophageal echocardiography(3D-TEE)in the applica?tion of percutaneous left atrial appendage occlusionin patients with atrial fibrillation.[Methods]7 patients with atrial fibrillation in Da?lian area(female 4,male 3,mean age 71.5 ± 3.3 years)were enrolled in the study.The maximum and minimum diameter and the max?imum depth ofleft atrial appendage were measured by 3D-TEE,and export morphology and leaf number were viewed.WATCHMAN occlude size was selectedby 3D-TEE. The process of operation was monitored by 3D-TEE. 3D-TEE was also performed in 3 months after the transcatheter closure.[Results]7 patients were performed the transcatheter closure therapy successfully. Preoperative 3D-TEE showed left atrial appendage export:5 cases were oval,and 2 cases were nearly circular. The leaf number was 1.6 ± 0.8. The maximum diameter of the left atrial appendage export was(23.1 ± 1.7)mm,(19.2 ± 2.1)mm minimum diameter,maximum depth of(27.6 ± 3.3)mm. The maximum diameter had good correlation with the size of occluder(r=0.944,P=0.001). 3D-TEE was used for guidance in the transcatheter therapy and succeeded. There were 1 case with little shunt after the transcatheter closure. 3 months af?ter the treatment ,position and shape of the occluderswere all in good.[Conclusion]3D-TEE has the great value in the percutaneous closure of left atrial appendage.

Objective To identify shortcomings in the current evaluation index system of medical quality. Methods The documentary analysis focused on the existing index system comprises the Hospital Management and Quality Evaluation Standards (2007) by China Hospital Association, Hospital Management Evaluation Guidelines (2005) by the Ministry of Health, and Implementation Rules of Hospital Management Evaluation Standards for Beijing (2007). JCI Accreditation Standards for Hospitals (edition 2) (2003), and New Hospital Accreditation Standards for Taiwan (2005, 2006) were referred to in the study. Results The shortcomings found include the weak operability of indexes, and the deficiency of evaluation indexes from the point of hospital visiting process by patients. Conclusion In view of the hospital visiting process of patients, add more indexes for patients' hospital visiting process,and quantize and highlight the operability of indexes, with summary and optimization of experiences in time by steps.

Objective To investigate the factors that induce constipation in bedridden patients undergoing replantation of severed finger and find countermeasures. Methods A retrospective investigation was carried out in 92 patients undergoing replantation of severed finger, their constipation and factors influencing constipation were recorded. Results 61 patients suffered from constipation in total 92 cases,the rate being 66.30%. 76 patients reduced food intake, and 59 cases presented constipation (the rate being 77.63%). Factors influencing constipation included being unaccustomed to defecate in bed (the rate that present with constipation being 87.09%),reluctant to trouble others(75.47%), side effects of drugs(55.56%) and lack of dietary knowledge (71.87%), decreasing food intake was closely bound up with constipation. Conclusions Proper psychological counseling, strengthening of health propaganda, comfortable facility for defecation, improved family support and oral food intake can significantly reduce the proportion of constipation and increase comfort degree of patients.