1.Correlation of adaptive skills with intelligence in young children
Chinese Journal of Tissue Engineering Research 2001;5(9):45-46
Objective To explore the relationship between adaptive skills and intelligence in young children. Method 30 young children aged from 3 to 6 years were concurrently administered the Adaptive Skill Rating Scale for Young Children(ASRSYC) and China- Wechsler Young Chilren Scale of Intelligence(C -WYCSI). Result The correlation coefficients between the total score of ASRSYC and the verbal scale score , performance scale score and full scale score of C - WYCSI were 0. 85, 0.70and 0. 81, respectively. The corrdlations between the total score of ASRSYC and the subtest scores of C - WYCSI ranged from 0. 51 to 0. 75 . Conclusion Adaptive skills and intelligenoe in young children relate each other and develop jointly.
2.Effects of blood transfusion on postoperative infection in patients with breast cancer
Xianqing ZHANG ; Ruoquan YAO ; Shijie MU
Journal of Clinical Surgery 2000;0(06):-
Objective To investigate the effects of blood transfusion on postoperative infection in patients with breast cancer.Methods 311 patients who underwent breast cancer operations in our hospital were observed.Among them,109 patients were transfused with red blood cell (RBC) concentrates,112 with white blood cell(WBC)-depleted RBC concentrates,90 were not transfused.The rates of postoperative infection of three groups were compared.Results The rate of postoperative infection of the group transfused with RBC concentrates was 5.50%.The rate of postoperative infection of the group transfused with WBC-depleted RBC concentrates was 0.89%,which was significantly different from that of the group transfused with RBC concentrates(P0.05).Conclusions In patients with breast cancer,the rate of postoperative infection of the group transfused with RBC concentrates was higher than that of group transfused with WBC-depleted RBC concentrates and of the group without transfusion. The rate of postoperative infection was closely correlated to the amount of RBC concentrates.
3.Study on mutation of mitochondrial gene from rat breast cancer
Pingzhong WANG ; Shijie MENG ; Pei LIU ; Yangzhen YAO
Chinese Journal of Pathophysiology 1986;0(04):-
AIM: To know the variations of the cytochrome b gene in cancer tissue, paracarinoma tissue and normal tissue and to inquire into the relationship between mutations of mitochondrial genome and carcinogenesis. METHODS: Cellular total DNA was extracted.The cytochrome b genes of three tissues were amplifyed with polymerase chain reaction(PCR). PCR products were analysed by DNA auto-sequencing method. RESULTS: The cytochrome b gene of cancer tissue had the C to G mutation at nt 14931, the C to G mutation at nt 15004 and the T to C mutation at nt15435,respectively. The cytochrome b gene of paracarinoma tissue had the A to C mutation at nt 15436. The cytochrome b gene of normal tissue had not mutation. CONCLUSION: Mitochondrial DNA mutations could be the endogenous factors that induce nuclear genome mutation. It could promoto carcinogenesis. The paracarinoma tissue was abnormal in DNA molecular level.
4.Relationship between inflammatory cytokines and traumatic brain injury
Zhansheng ZHU ; Shijie CHEN ; Mingcan WU ; Yuan YAO
International Journal of Surgery 2010;37(10):700-702
A medical studies have indicated that there would be a defense response from human body after TBI,which mainly comes with inflammatory reaction. Human body's resistance seems likely to be enhanced,during this process, by inflammatory cytokines leading to rehabilitation of the cellular organization.However,a strong Inflammation from the whole human body may be caused due to the excessive activation,which, instead, worsens secondary brain injury. This article discusses the relationship between cytokines and traumatic brain injury.
5.Significances of Up-expressions of CYCLIND1 and CDK4 in pancreatic carcinoma
Yong YANG ; Yunning HUANG ; Gang SU ; Shijie YANG ; Baoguo YAO
Basic & Clinical Medicine 2006;0(07):-
Objective To investigate the expressions of CYCLIND1 CDK4 in pancreatic cancer and their relationship with clinlicopathological parameters.Methods Immunohistochemical detector with new second generational tow steps method was used to detect the expressions of CYCLIND1 CDK4 and PCNA in 48 cases of pancreatic cancer and 14 cases of chronic pancreatitis.Results The expressions of CYCLIND1 CDK4 proteins in pancreatic cancer were 70.85%(34/48)and 62.50%(30/48) respectively.The CYCLIND1 and CDK4 proteins were increased(P
6.Expression of Notch4 in renal cell carcinoma and its relationship with the microvascular density
Liang PANG ; Guangming LIU ; Wenli SONG ; Zhen JING ; Shijie YAO
The Journal of Practical Medicine 2017;33(15):2525-2529
Objective To investigate the expression of Notch4 protein and to analyze its correlation with the clinical parameters and the microvessel dentisty in renal cell carcinoma. Methods The expression of Notch4 was examined in 60 cases of renal cell carcinoma and the para-carcinoma tissue by SP immunohistochemical stain-ing ,and CD34 detection was used for counting microvessel density. Statistical analysis was performed to reveal the correlation with clinicopathological parameters ,microvessel density and prognosis. Results The positive rate of Notch4 protein expression was 75%(45/60)in para-carcinoma tissue,and was 43.3%(26/60)in renal cell car-cinoma,with significant difference on tumor grade and Lymph node metastasis(P<0.05). The microvessel densi-ty in Notch4 positive tissues was significant lower than that in the negative samples(P<0.05). The survival time of patients with Notch4 positive expression was significantly longer than that of patients with Notch4 negative expres-sion(P<0.05). Conclusion Notch4 protein plays an important role in the development of renal cell carcinoma. Notch4 expression might both attenuate the malignant biological characteristics and suppress the angiogenesis dur-ing tumor development.
7.Comparison of Three Treatment Methods for Cholecystolithiasis with Commom Bile Duct Stones
Shijie ZHONG ; Tiehan LI ; Lei ZHU ; Yong HOU ; Jun HU ; Hanlin YAO ; Hong ZHU
Journal of Kunming Medical University 2016;37(10):81-84
Objective To summarize the clinical experience of three different treatments for gallbladder and common bile duct stones.Methods The clinical data of 180 cases of gallbladder stones combined with bile duct stones undergoing surgery from May 2010 to May 2013 were retrospectively analyzed.They were divided into three groups,A group of 60 patients underwent a period of endoscopic sphincterotomy (EST),under the second phase of laparoscopic cholecystectomy (LC);Group B 60 patients underwent laparoscopic cholecystectomy (LC) + laparoscopic common bile duct exploration surgery (LCBDE) + T tube drainage;Group C 60 patients underwent conventional open cholecystectomy (OC) + bile duct exploration (OCBDE) + T tube drainage.Results A group of 53 cases completed surgery successfully,5 cases of remaining 7 patients failured in the first phase surgery,2 cases of the 5 patients did LC + LCBDE,3 of the 5 patients underwent conventional surgery.Two patients underwent the conventional surgery in the second phase surgery.B group of 57 cases completed surgery successfully,three cases convert to open surgery.All of the group C completed the surgery successfully.Group A complication was the most in the three groups (P<0.05);group B had the shortest time of hospitalization (P<0.05),the complication rate was lower than that in group A (P<0.05),the complication had no significant difference between A and B.(P and group B > 0.05),group B had shortest operation time (P<0.05);no statistically significant differences were found among three groups in fasting time.Conclusion Three treatment methods have advantages as well as disadvantages,a reasonable treatment should be selected according to the specific circumstances.
8.Assessment of community health service ability of general practitioners after standardized training in Shanghai
Hui LI ; Minghui PENG ; Shijie FU ; Xiangjie ZHANG ; Yao LIU ; Sunfang JIANG
Chinese Journal of General Practitioners 2016;15(9):672-676
Objective To assess community health service ability of general practitioners (GPs) with the Standardized General Practitioner Training(SGPT) in Shanghai.Methods One hundred and four GPs receiving SGPT and matched 102 GPs without SGPT were enrolled in this retrospective cohort study from September to November in 2014.Community health service ability,including abilities in basic medical service and public health service were assessed and compared between two groups.Written examination was applied in the public health services ability assessment.Objective structured clinical examination (OSCE) was applied for evaluation of basic medical service abilities.Results The scores of written examination in GPs with SGPT were higher than those without SGPT[(66 ±4) vs.(62 ±7),P <0.01].The OSCE scores in GP with SGPT were higher than those without SGPT [(494 ± 78) vs.(448 ± 80),P < 0.01].GPs with SGPT also abtained the higher scores in consultation competence and clinical skills assessment [(245 ± 44) vs.(225 ±40),P<0.01;(252 ±59) vs.(225 ±61),P <0.01].In OSCE,GPs with SGPT got higher score than those without SGPT in 5 stations (medical history collection,doctor-patient communication,basic operation,ECG interpretation,and CPR) (all P < 0.01);however,no significant differences were observed in 3 stations (physical examination,case analysis and X-ray interpretation) between two groups (all P > 0.05).Conclusions GPs with SGPT have better basic medical service and public health service ability.But some aspects of basic medical service ability need to be strengthened in the training and practice.
9.Development of urothelial tumors following renal transplantation of 11 cases report
Wenhui SONG ; Zhijie BAI ; Shijie YAO ; Qian HU ; Haifeng WANG ; Qingtong MA ; Shiqiang YANG ; Hongshun MA
Clinical Medicine of China 2012;28(5):528-530
Objective To analyze the incidence and clinical features of urothelial tumors in renal allograft recipients.Methods A retrospective analysis of 1042 patients received renal allografts who had taken immunosuppression for at least six months between 2006 and 2011 in The First Centre Hospital of Tianjin was performed.Results Eleven cases of uroepithelial tumors were diagnosed in the 1042 cases of renal transplantation ( 1.06% ),of whom 9 cases were noticed by hematuria ( 81.8 % ),2 cases ( 18.2% ) by medical examination.Six patients were diagnosed with multifocal urothelial carcinomas.Surgery was performed on all the patients with renal tumors and followed by chemotherapy or radiotherapy.Conclusion Malignancies in urinary tract after renal transplantation should be bore in mind.Early diagnosis is very important.The treatment options include reducing immunosuppressive agents and removing tumor lesions completely.
10.Expression of Oct4 and Wnt2 in human glioma tissues and its clinical significance
Guangrui ZHAO ; Mingcan WU ; Shijie CHEN ; Junchuan LI ; Tingxuan CHEN ; Yuan YAO
Chinese Journal of Cancer Biotherapy 2009;16(6):629-632
Objective:To investigate the expression of Oct4 and Wnt2 in human glioma tissues and its relationship with the clinicopathological features of glioma. Methods: Fifty-six paraffin blocks were obtained from glioma patients receiving surgery. The diagnosis of these patients were confirmed by pathology in our hospital from 2006-2009. Immunohistochemi-cal staining was used to examine Oct4 and Wnt2 expression in the brain tissues of 10 patients with acute brain injury and 56 glioma tissues (including 15 recurrent cases). Results: The normal brain tissues were negative of Oct4, with only one case showing weak Wnt2 expression. Thirty-four of the 56 glioma tissues showed positive expression of Oct4 (60.7%), and 40 showed positive expression of Wnt2 (71.4%). Positive expression rates of Oct4 and Wnt2 in low-grade and high-grade glioma tissues were 46.2 %, 73.3% and 57.7 %, 83.3%, respectively (P < 0.05). Oct4 positive rates in the recrudescence and newly diagnosed glioma tissues were 86.7% and 51.2%, respectively (P < 0.05). Oct4 expression in the glioma tissues was positively correlated with that of Wnt2 (r = 0.537, P < 0.01). Conclusion: Expression of Oct4 and Wnt2 is associated with the malignant degrees of glioma, and Oct4 expression is related to the recurrence of glioma. Oct4 might participate in the development and progression of brain glioma through Wnt signaling pathway.