Objective:To test the periodontal regeneration potential after tooth root replantation combined with in vitro cultured periodontal ligament cells (PDLCs).Methods:The third and fourth mandibular premolars of two mongrel dogs were extracted and the dog PDLCs were in vitro cultured.The autologous roots combined with PDLCs were replanted into the created cavities in the right mandibular edentulous area one month after premolar extraction.Roots replanted without PDLCs on the left mandible served as the controls. The dogs were sacrificed 2 months after the replantation. Paraffin sections were made and stained with hematoxylin & eosin, then examined histologically.Results:In the samples of root combined with PDLCs implantation, some fiber bundles vertical to the surface of bone were embedded in some inner walls of the alveolar socket opposite to the apical root.Conclusion:Cultured PDLCs in vitro retain the capacity of PDL-like tissue formation even in the artificial alveolar socket.

This paper presents an analysis of Professor LuoYuankai from diagnosis and treatment of anovulatory infertility from kidney, and have preliminarily summarized the various clinical experience of kidney diseases therapeutic methods. Through the discussion of the famous prescription, thoughts of comorbidity by different treatments and characteristics of medicine uses, in order to provide reference for the clinical treatment thus improve clinical curative effect.

Objective To analyze the efficacy of repairing unilateral cleft lip (UCL) with Millard method, and explore the individual operative procedure. Methods According to the difference of lip height between non-cleft and cleft sides, MillardⅠ, MillardⅡ or modified Millard Ⅱ operative approach was chosen to repair UCL, then the clinical effect of operation was evaluated. Results The ideal operative effects were obtained in 208 cases of UCL using various operative approaches based on the individual deformity. There were consistent lip height between both sides, normal vermillion bulk and satisfactory facial appearance after operation. Conclusion The satisfactory efficacy could be obtained by choosing Millard method's operative approaches based on the individual deformity. The modified Millard operative approach could repair all kinds of UCL.

Objective To investigate the proliferation and apoptosis in stage-ⅠNSCLC and their prognostic implications.Methods Immunohistochemical technology and TUNEL assay was applied to detect proliferation and apoptosis in 118 lung cancer tissues and 30 normal lung tissues as controls.Results Proliferation Index(PI)was up-regulated in lung cancer tissues compared with that in normal lung tissues and was closely related to T-staging,differentiation and smoking.Apoptotic Index(AI)was up-regulated in lung cancer tissues compared with that in normal lung tissues and was closely related to T-staging.PI was not related to AI in stage-ⅠNSCLC.The patients with high PI had a shorter 5-year survival than those with low PI(33.57% and 73.12%,P=0.0001),and the patients with low AI had a shorter 5-year survival than those with high AI(41.48% and 68.80%,P=0.008).Multivariate analysis showed that PI was a significantly independently predictive factor for patients with stage-ⅠNSCLC(RR=2.473,95%CI:1.278~4.784,P=0.007).Conclusion Stage-ⅠNSCLC is a subgroup with high proliferation and apoptosis.Proliferation plays a more important role in stage-ⅠNSCLC.The assessment of proliferation and apoptosis may provide new insight into prognosis and adjuvant treatment for stage-Ⅰ NSCLC.

Objective:To investigate the effect of Shuanghuangbu slow releasing strip (SRSS) on the expression of BMP2 and IGF1 in the experimental regeneration of periodontium. Methods:Periodontal defects were surgically made around the second incisor and the ca nine in four beagle dogs .32 defects were randomly divided into experimental gr oup and control group with 16 defects in each group. SRSS was applied to the e xperimental group and no disposition to periodontal defect in the control group. Two teeth of each dog were randomly chosen as the natural controls.Specimens we re respectively obtained one month and three months after operation. BMP2 and I GF1 were quantitatively analyzed by immunohistochemistry. Results:The positive rate of BMP2 and IGF1 in experimental group were higher t han those in the control( P

The article briefly reviews the aged lung cancer in epidemiology, physical and pathologic traits, and treatments. It includes the matters needing attention of operation with elder lung cancer patients; matters about the radiotherapy; choice of chemotherapeutics or dosage; situation of targeted drug and treatment of Chinese Medicine. Goals of treatment with elder lung cancer patients are prolonging lifetime, improving quality of life. Patients should be treated individually according to their state of illness and constitutions.

In order to meet the requirements of ultrasound bone density measurement, we designed a sofware based on Visual Studio C+ + 2008. The software includes interface design, acquisition and control, data processing and parameter extraction, data storage and printing. Excellent human-computer interface (HCI) will give users a convenient experience. Auto gain control (AGC) and digital filter can improve the precision effectively. In addition, we can observe waveform clearly in real time. By using USB communication, we can send control commands to the acquisition and get data effectively, which can shorten the measuring time. Then we calculated the speed of sound (SOS) and broadband ultrasound attenuation (BUA). Patients' information can be accessed by using XML document. Finally, the software offers printing function.
Absorptiometry, Photon ; instrumentation ; Bone Density ; Humans ; Information Storage and Retrieval ; Software ; Sound ; Ultrasonics ; methods ; User-Computer Interface

Plague, infectious disease in modern medicine, refers to a type of disease with strong pathogenicity and infectiousness, it refers to the infectious diseases of western medicine. Due to its wide variety, the knowledge and understanding of plagues of Traditional Chinese Medicine (TCM) doctors in different stages have evolved and developed with the times. This article, via collating ancient documents, differentiatesthe classification in TCM and analyzes itsrelated theories to perfect the type of plagues in TCM, providing the theoretical basis for the research of plague in modern times.

Objective To evaluate the efficacy of combined anterior and posterior approaches in treatment of severely unstable lower lumbar burst fractures.Methods A retrospective case series study was made on clinical data of 14 patients with lower lumbar burst fractures collected from August 2009 to August 2014.There were 12 males and 2 females,with a mean age of 39 years.Seven fractures occurred at L3,five at L4,and two at L5.Nine patients were associated with injury to the posterior ligament complex (PLC).According to the American Spinal Injury Association (ASIA) classification,the spinal injuries were rated as grade B in two patients,grade C in four,grade D in five and grade E in three.All patients underwent posterior pedicle screw fixation combined with anterior spinal canal decompression and titanium mesh or iliac bone grafting.Lumbar lordosis angle,vertebral height,spinal canal decompression,ASIA grade and complications were evaluated after operation.Results Two patients experienced cerebrospinal fluid leakage postoperatively,which were healed after 2 weeks' local pressure treatment.Three patients experienced recurrent lumbar pain postoperatively,which were relieved after the removal of internal fixation 18 months after operation.All patients were followed up for 12-36 months (mean,18 months).Compared to the detection before operation,final follow-up showed improved lumbar lordosis [(30.2 ± 7.3) ° vs.(41.3 ± 6.5) °],decreased loss of the anterior vertebral height [(62.3 ± 21.5) ％ vs.(11.8 ± 7.8) ％] and reduced canal compromise [(65.7 ± 30.5) ％ vs.(21.9 ± 12.7)％] (all P ＜ 0.05).ASIA grade was significantly improved at the final follow-up,including grade C in one patient,grade D in three and grade E in ten (P ＜ 0.05).Follow-up showed no apparent graft loosening,pseudarthrosis,implant breakage and severe kyphosis.Conclusion Combined anterior and posterior approaches to treat severely unstable lower lumbar burst fractures can effectively reconstruct the height and stability of the vertebral body,restore the spinal canal volume,and attain satisfactory clinical outcome.

The origin and the course of the thoracic duct and its opening into the vein were studied in 150 cadavers including 15 fetuses and 138 infants. They are classfied into 5 types.1. The normol type of the thoracic duct begins in the abdominal cavity as a single trunk, It ascends along the right side of the aorta and empties into the venous system on the left side at the root of the neck. This type of the duct which is described in the general text book, occurred in 84.67 per cent.2. The two-trunk type of the thoracic duct begins in the abdominal cavity as two trunks and ascends along the each side of the aorta. The two trunks join together at different levels in the thorax forming a single trunk which empties into the venous system on the left side at the root of the neck. This type of the duct occurred in 10.66 per cent.3. The bifurcated-type of the thoracic duct starts in the abdominal cavity as a single trunk and passing cephalad on the right side of the aorta and divides into two branches at the level of the 6th～4th thoracic vertebra, The right branch opens into the venous svstem on the right side and the left branch opens into the venous system on the left side. This type of the duct occurred in 3.33 per cent. The type which ascends along the left side of the aorta was found.4. The right thoracic duct begins in the abdominal cavity as a single trunk and runs its entire course along the left side of the aorta. The termination opens into the right jugular venous angle. This type of the duct occurred in 0.67 per cent.5. The left thoracic duct begins in the abdominal cavity as a single trunk and ascends along the left side of the aorta. It empties into the left jugular venous angle. This type of the duct occurred in 0.67 per cent.