Objective To analyse the clinical significance of variable deceleration and extended deceleration in non-stress test. Methods Studied the clinical characteristics of variable deceleration and extended deceleration of 200 cases who underwent non-stress test from January 2005 to December 2007, and compared corresponding clinical significance and newborn prognosis. Results Variable deceleration and extended deceleration in non-stress test dued to cord entanglement, polyhydramnios and fetal growth restriction. The occurrence of fetal distress and severe asphyxia in nowbom were less in low-grade variable deceleration (8.3%, 1.0%) than those in high-grade variable deceleration (17.7%, 4.8%)and extended deceleration (23.8%, 7.1%). Condusion Variable deceleration and extended deceleration in non-stress test due to cord entanglement mostly, low-grade variable deceleration is not always clue to fetal distress and high-grade variable deceleration and extended deceleration is often clue to fetal distress.

OBJECTIVE:To establish the quality standard for Qindan granules. METHODS: The constituents including Radix Scutellariae, Radix Astragali, Cortex Moutan and Fructus Gardeniae in Qindan granules were identified respectively by TLC. RESULTS: The test samples and their respective reference substances were alike in that the same color chromatographic spots were noted at the corresponding places. The color spectra of the samples were clear yet without interference of surrounding impurity. CONCLUSION: The established method has high specificity and can be used for the quality control of Qindan granule.

Liver tissues around hydatid from 8 patients with cystic echinococcosis were observed by transmission electron microscopy,normal hepatic tissues of 6 cases were used as control.The results demonstrated predominant atrophy and necrosis of hepatocytes.These changes seem to be the major hepatic lesion in cystic echinococcosis.

This study retrospectively reviewed 9 cases of complicated hepatic cystic hydatidosis with intrabiliary rupture who were surgically treated with pericystectomy in combination with Roux-en-Y hepaticojejunostomy in our hospital from 2004 to 2010. The clinical features, results of laboratory tests, B-mode ultrasonography and CT, post-operative recovery, days of hospital stay after the operation and post-operative complications were statistically analyzed and the patients were followed up. The subjects in our series included 7 males and 2 females, whose average age was 50.78±7.58 years. Before operation, 9 patients suffered from pain of the right upper quadrant and jaundice, which, in 4 cases (44.45%), were accompanied with fever and chills. Preoperative B-mode ultrosonography and CT showed that all the 9 patients had single hydatid cyst, with their diameter being 9.33±1.58 cm on average. The lesions involved segments V, VI in 6 cases, and segment IV in 3 cases. By WHO classification, 7 cases were classified as CE3 and 2 cases as CE4. They all had choledochectasia. The subjects underwent the surgery uneventfully. Intraoperatively, 2-4 biliary fistula orifices were found, with the average of the orifice being (0.79±0.20) cm. After the operation, one patient developed incision infection, one had pulmonary infection and one suffered from reflux cholangitis. No anastomotic leaks or peri-operative deaths took place and follow-up revealed no recurrence and implantative metastasis. We are led to conclude that pericystectomy in combination with Roux-en-Y hepaticojejunostomy can achieve satisfactory results for the treatment of complicated hepatic cystic hydatidosis with intrabiliary rupture.

BACKGROUND:Human amniotic epithelial cells are an important source of cells in regenerative medicine as its multipotentation, but new studies mainly focused on differentiation features and there were little research oneffect of culture in vitro on biological property of amniotic epithelial cells.
OBJECTIVE:To analyze the effects of in vitro culture on growth, cellphenotype and differentiation capacity of human amniotic epithelial cells into cardiomyocyte-like cells, and explore the correlation of primarily cultured human amniotic epithelial cells marker SSEA-4 expression level and the change of biological characteristics of human amniotic epithelial cells.
METHODS:Primarily cultured human amniotic epithelial cells were obtained from amniotic tissues by using the same separation protocol. Human amniotic epithelial cells were cultured in vitro. The proliferation, cellphenotype and the differentiation capacity of human amniotic epithelial cells into cardiomyocyte-like cells were evaluated by means of cellcounting kit-8, flow cytometry and real-time PCR.
RESULTS AND CONCLUSION:The SSEA-4 positive cells in primarily cultured human amniotic epithelial cells from different fetal tissues were between 26.7%-97%, which indicated that there was great individual difference among amniotic tissue samples. Moreover, with passage, the SSEA-4 expression in human amniotic epithelial cells decreased significantly, which did not correlate with the SSEA-4 expression in primarily cultured human amniotic epithelial cells. Results indicated that there was great individual difference in SSEA-4 expression level in primarily cultured human amniotic epithelial cells from different amniotic tissue samples. Thus, it is necessary to set up clinical screening indexes to get samples with higher SSEA-4 expression stably and to control the quality of human amniotic epithelial cells. In addition, during culture period, SSEA-4 expression level was affected by culture conditions. The culture conditions of human amniotic epithelial cells should be optimized to maintain SSEA-4 expression at a high level. In addition, the differentiation capacity of human amniotic epithelial cells into cardiomyocyte-like cells was also affected by individual difference among different samples and culture conditions, which wil be further studied in the future.

This study retrospectively reviewed 9 cases of complicated hepatic cystic hydatidosis with intrabiliary rupture who were surgically treated with pericystectomy in combination with Roux-en-Y hepaticojejunostomy in our hospital from 2004 to 2010.The clinical features,results of laboratory tests,B-mode ultrasonography and CT,post-operative recovery,days of hospital stay after the operation and post-operative complications were statistically analyzed and the patients were followed up.The subjects in our series included 7 males and 2 females,whose average age was 50.78±7.58 years.Before operation,9 patients suffered from pain of the right upper quadrant and jaundice,which,in 4 cases (44.45％),were accompanied with fever and chills.Preoperative B-mode ultrosonography and CT showed that all the 9 patients had single hydatid cyst,with their diameter being 9.33± 1.58 cm on average.The lesions involved segments V,Ⅵ in 6 cases,and segment Ⅵin 3 cases.By WHO classification,7 cases were classified as CE3 and 2 cases as CE4.They all had choledochectasia.The subjects underwent the surgery uneventfully.Intraoperatively,2-4 biliary fistula orifices were found,with the average of the orifice being (0.79±0.20) cm.After the operation,one patient developed incision infection,one had pulmonary infection and one suffered from reflux cholangitis.No anastomotic leaks or peri-operative deaths took place and follow-up revealed no recurrence and implantative metastasis.We are led to conclude that pericystectomy in combination with Roux-en-Y hepaticojejunostomy can achieve satisfactory results for the treatment of complicated hepatic cystic hydatidosis with intrabiliary rupture.

Objective To compare and analyze the effect of membrane element and spring element on biomechanical responses of cervical ligaments. Methods Based on the existing 6-year-old pediatric neck finite element model, the ligaments were simulated by membrane element and spring element, respectively. Then dynamic tensile test of C4-5 vertebrae and tensile test of full cervical spine were conducted. The membrane element model was also used to simulate the bending test, and the simulation results were analyzed. Results In dynamic tensile test of C4-5 vertebral segment, the final failure force of membrane element simulation test and spring element simulation test was 1 207 N and 842 N, respectively, and their difference from the cadaver experiment was 0.6% and 30.6%, respectively. In full cervical tensile test, the difference of peak force from membrane element simulation test and cadaver experiment was 1.8%. The peak force of spring element simulation test was 484 N, and the difference from simulation test and cadaver experiment was large. The simulation result of membrane element bending test was good. Conclusions The spring element had some limitations in force simulation. The membrane element had higher biofidelity and could reflect the biomechanical response of the ligaments.

Objective To explore the effects of different skull-brain interfaces and mesh density of the cerebrospinal fluid (CSF) on dynamic responses of the brain. Methods The impact kinematics on cadaver head under rotation and translation impacts were reconstructed based on the 50th percentile adult head finite element model. The interfaces between skull and CSF, CSF and brain were modeled with different types of interfaces, which were set as sharing nodes, tied, frictionless sliding, so as to investigate the effect of different interface types on dynamic responses of the brain. Then, the interfaces between CSF, skull and brain were set as sharing nodes, while CSF was divided into single-layer and tri-layer of hexahedral element with the constant thickness of CSF, to study influences of CSF with different mesh density layers on dynamic responses of the brain. Results The intracranial pressure was highly sensitive to the interface types, while the brain response seemed to be relatively insensitive to the variation in CSF layers. Conclusions The research findings provide theoretical references for the construction of CSF and the selection of skull-brain contact interface of the head finite element model.

Modern vehicle safety design and safety regulations are mostly based on 50th percentile populations. However, with the increase of obese populations, it is very important to investigate the injury mechanism and protection of obese occupant. Methods such as traffic accidents statistics, cadaver experiments, multi-body modeling and finite element modeling, are currently used to study the injury mechanism of obese occupants. Different hypotheses including cushion effect, body geometrical effect and mass increasing effect have been put forward to explain the effect of obesity on occupant injury mechanism, which means that its mechanism is still uncertain. The impact injury mechanisms of obese occupant were comprehensively summarized. Furthermore, the problems confronted by the research of current obese occupant impact injury and future investigations were proposed in this study.

Objective To study the effect of neck restrain on traumatic brain injury during airbag inflation in traffic accidents. Methods Based on the previously validated 3-year-old child head finite element (FE) model, the impact on out-of-position (OOP) child occupant during airbag inflation was simulated by FE method, so as to investigate the effects of neck restraint on intracranial response and injury mechanism in traffic accidents. Results The head kinematics with neck restrain was different from that without neck restrain under the impact of airbag inflation. The neck restraint would obviously decrease the maximum Von Mises stress of pediatric brain. When airbag-head distance was 20 cm or 25 cm, the neck restraint would obviously decrease the maximum intracranial pressure. Conclusions Neck restraint had a relatively large influence on pediatric intracranial response. When the FE method is used to predict pediatric craniocerebral injury, consideration of neck restrain on child brain response is necessary.