The purpose of the present study is to establish a rapid and effective PCR method for the identification of B. multicinctus. Based on sequence alignment of B. multicinctus and its adulterants, we found that Cyt b gene is a good molecular genetic marker for the authentication of B. multicinctus. On the basis of the sequence data, a pair of highly specialized primers was designed. The templates were extracted by the DNA purification system. Key factors such as annealing temperature, concentration of Taq enzyme and cycle numbers were analyzed and optimized. The modified PCR program consisted of an initial denaturation step at 95 degrees C for 5 min, followed by 30 cycles of 95 degrees C for 30 s and 55 degrees C for 45 s and a final extension at 72 degrees C for 5 min. Thirteen samples of B. multicinctus were identified accurately from their 20 adulterants in 4 hours. The results indicated it is a highly accurate, rapid and applicable method for the authentication of B. multicinctus.

Nasopharyngeal carcinoma (NPC), a common head and neck neoplasm in southern China,is mostly treated with radiotherapy, however, it can not be completely cured by chemoradiotherapy. Recent investigation on NPC suggests that the nasopharyngeal carcinoma stem cells can be differentiated to heterogeneous cancer cells that have a strong ability for self renovation and further contribute to the development and progression of tumor itself which is closely related to drug resistance, recurrence and metastasis of NPC. Wnt/β-catenin, Notch, Hedgehog, NF-κB and mTOR signaling pathways play important roles in cancer stem cells. Study on nasopharyngeal carcinoma stem cells targeted therapies and its related pathways provides a new strategy for the clinical treatment of NPC.

Objective To analyze the composition principles of Chinese patent drugs containing Lonicerae Japonicae Flos (Jinyinhua) in National Standard for Chinese Patent Drugs (NSCPD) enacted by Ministry of Public Health of China by using Traditional Chinese Medicine Inheritance Support System (TCMISS), and provide reference for its clinical application and development. Methods Based on TCMISS (V2.0), with methods of association rules and improved mutual information method, we analyzed the composition patterns and rules of single drugs and drug combinations. Results Through analyzing on the 201 patent drugs, 21 core drug combinations and 19 diseases that Jinyinhua commonly used were mined, from which influenza, and sore and ulcer were chosen to further analysis, including core drugs and drug combination rules, syndrome etc, disclosing the composition principles of Jinyinhua used in different diseases. Conclusion Patent drugs containing Jinyinhua collected from NSCPD mainly could clear heat and remove toxicity, and the composition principles reflect the compitable characteristics of Jinyinhua in treating different diseases, which are in accordence with clinical application of multiple-efficacy drugs.

Objective To explore the effects of community-based comprehensive intervention on social function and quality of life of patients with bipolar disorder. Methods 244 bipolar disorder patients were assigned into control group (n=119) and intervention group (n=125). The control group accepted antipsychotics only, and the intervention group accepted community-based comprehensive intervention in addition. They were assessed with Social Disability Screening Schedule (SDSS) and Generic Quality of Life Inventory-74 (GQOLI-74) before and 6, 12 months after treatment. Results The SDSS score was lower (P<0.05) and the GQOLI-74 score was higher (P<0.05) in the intervention group than in the control group. Conclusion Community-based comprehensive intervention can improve the social function and quality of life of patients with bipolar disorder.

Objective To discuss the medication regularity of prescriptions containing Asini Corii Colla;To provide data support for clinical compatibility application of Asini Corii Colla.Methods Totally 2635 articles about prescriptions containing Asini Corii Colla were retrieved from CNKI from Jan. 1995 to Dec. 2012. TCM Inheritance Support System V2.0 was employed to conduct frequency analysis and association rules analysis, with a purpose to determine the frequency, indications of disease, indications of syndrome and medicine core combination of common prescriptions containing Asini Corii Colla.Results The 2635 prescriptions containing Asini Corii Colla involved 1197 Chinese herbal medicines, 34 kinds of which were used in extremely high frequency (frequency>200). 30 kinds of indications of disease were treated in the high frequency (frequency>20). 32 kinds of indications of syndrome were treated in the high frequency (frequency>20). The combination of Glycyrrhizae Radix et Rhizoma, Angelicae Sinensis Radix and Asini Corii Colla showed the highest frequency in all three herbal combinations (486 times), and Atractylodix Macrocephalae Rhizoma, Asini Corii Colla, Codonopsis Radix and Astragali Radix showed the highest frequency in all four herbal combinations (272 times).Conclusion Most of compatibility them can enrich blood and tonify qi, and have major functions for gynecological diseases and major syndromes of qi-blood deficiency.

Skin health is an important aspect of cosmetology. With the increase of social demand， traditional Chinese medicine （TCM） brings its advantages into play in health care and conservation. Under the guidance of Xiang thinking， the method of "treating disease with color" is formed， which is also known as TCM color therapy. It refers to a method of treating diseases with Chinese medicines of opposite or interpromoting color to skin lesions， which reflects the unique thinking and diagnosis and treatment methods of TCM， and has practical value in the field of skin health. This study outlined the formation and application of "treating disease with color"， and summarized and analyzed the classic prescriptions and their composition characteristics in "treating the black with white" and "treating the white with black". Common medicinal materials in "treating the black with white" included Angelicae Dahuricae Radix， Ampelopsis Radix， Poria， Atractylodis Macrocephalae Rhizoma， Bletillae Rhizoma， and Typhonii Rhizoma， and those in "treating the white with black" included Polygoni Multiflori Caulis， Rehmanniae Radix， Sesami Semen Nigrum， Ecliptae Herba， Ligustri Lucidi Fructus， and Mori fructus. On this basis， "treating disease with color" was proposed as a characteristic treatment method for skin health care and conservation， which would effectively guide the research and development of products related to healthy food， cosmetics， healthcare food， and new Chinese medicine. In addition， this paper conducted a detailed elaboration from the following three perspectives： further exploring the theoretical connotation of "treating disease with color"， strengthening the basic research of the causes and effects of medicinal color， and promoting the research and development of skin health products under the guidance of "treating disease with color". It provided reference for the research and development of TCM skin health products.

OBJECTIVEUsing complex system entropy method to analyze composing principle of anti-phthisis prescriptions.

METHODCollecting prescriptions for anti-phthisis and constructing database for it. Using revised mutual information, complex system entropy cluster to analyze association between herbs, core combinations of prescriptions.

RESULTBased on analysis of 477 cases of prescriptions, the frequency of each herb in the database is computed. Moreover, 36 frequently used combinations of herbs and 34 core combinations are mined from the database by complex system entropy method.

CONCLUSIONComplex system entropy method is able to mine composing principle of prescriptions.

Databases, Factual ; Drug Prescriptions ; Medical Informatics ; methods ; Tuberculosis, Pulmonary ; drug therapy

Nature of Chinese materia medica is the nucleus in the theory of Chinese material medica, according to the recognition of Traditional Chinese Medicine, which is the character of the drug related on curative effect. Nature and efficacy of a drug is through the medical material, then, physical environment, including the temperature, humidity, atmospheric water, wind, topography, soil, micro-organism, and so on, influence the growth and development of the medical meterial. In this paper, we researched the explanation on nature of Chinese materia medica in the medical books of past dynasties, combined with the modem research, analyzed the relationship between generative reception and physical environmental factors, and discussed the effect of physical environmental factors on nature of Chinese materia medica. We indicated that the formation of Chinese materia medical nature is that the drug receptive the change of physical environmental factors, and resulted by the synthetic action of the factors, such as climate, soil, biology, topography, etc.
Environment ; Humans ; Materia Medica ; Medicine, Chinese Traditional

OBJECTIVETo establish a convenient, practical and high efficient method of DNA extraction of os cervi, and lay the foundation of identification of animal bones.

METHODThe bones of sika deer, red deer, cattle, dog and pig were used to extract DNA under different decalcification time (24,48,72 h) and decalcification temperature (4,25,37,56,70 degrees C), and extract method.

RESULTIt proved by experiments that demineralization process promotes the cracking of osteocyte. In a broad of decalcification time and temperature, DNA could be extracted from all bone samples successfully while the quantity varied slightly.

CONCLUSIONSamples (about 0.1 g) decalcify with 0. mol x L(-1) EDTA at 4 degrees C for 24 h, then water-bath for 1 h after lysis buffer added, DNA extracted via the method above is of high quality and can be used for PCR.

Animals ; Bone Demineralization Technique ; Bone and Bones ; chemistry ; metabolism ; Cattle ; DNA ; isolation & purification ; Deer ; Dogs ; Polymerase Chain Reaction ; Swine ; Temperature ; Time Factors

This paper summarized and analyzed the status quo and problems about molecular identification of animal medical material, based on the facts, we proposed some research strategies, including uniting to tackle key problems, expanding the research species, accelerating manufacture and generalization of molecular identification kit, priming the research project of DNA barcoding, and establishing standard database on animal medical material.
Animal Structures ; Animals ; DNA Barcoding, Taxonomic ; Databases, Genetic ; standards ; Materia Medica ; analysis ; Mitochondria ; genetics ; Research