Objective The study aimed to explore the relationship between storage methods and pollution degrees of two different storage methods for medical devices which can not be packed promptly after cleaning.Methods 300 pieces of surgical devices were randomly divided into the experimental group and the control group according to random digit table,150 pieces in each group.Each group was washed with two cleaning machines of the same brand and model number using the same cleaning protocol,and after cleaning,the experimental group was stored in the cleaning machine,while the control group was exposed in the packaging area.Ten hours later,each surgical instrument was detected using ATP biological fluorescence detector.Results The percent of pass in the experimental group was higher than that of the control group [98％ (147/150) vs.86％ (129/150)],x2=12.7,P＜ 0.05.Conclusions Medical devices should better be stored in the washing machine than exposure to the air after cleaning if they could not be packaged and sterilized immediately.

To study the clinical feature and surgical outcome of subretina and vitreous hemorrhage associated with senile macular degeneration (SMD). Vitrectomy was performed in 29 eyes of 29 patients with subretina and vitreous hemorrhage associated with SMD. Changes in the retina were observed, and the results of surgery were analyzed. The results showed that there was submacular hemorrhage in 16 patients, in whom 4 showed periomacular subretinal hematoma. There was peri macular subretinal hematoma only in 13 cases. The pathological changes in the macular region included discoid degeneration, submacular neovascularization, fixed foldings, exudation, scar and atrophic discoid cicatrization. All patients got visual acuity improvement after surgery. However those patients who had subretinal hemorrhage got less improvement of visual acuity than the others. It suggested that the SMD patients who was complicated by subretinal hemorrhage had a severer macular lesion. Pars plana vitrectomy could restore central vision of patients who suffered from subretinal and vitreous hemorrhage. But the outcome was related with the position of subretinal hemorrhage.

Objective To study the non-iridoid constituents from the roots of Gentiana straminea.Methods The compounds were repeatedly separated and purified on column chromatography of silica gel,Sephadex LH-20,and ODS,and their structures were identified on the basis of spectral and chemical methods.Results Twelve compounds were isolated from 70% ethanol extract of G.straminea and were identified as(-)-syringaresinol-4,4′-bis-?-O-D-glucopyranoside(1),gentiaphyllide-D(2),erythrecentaurin(3),macrophyiioside D(4),gentiaphyllide-E(5),roburic acid(6),uvaol(7),?-sitosterol(8),daucosterol(9),gentianose(10),?-glucose(11),and ?-glucose(12),respectively.Conclusion Compounds 1-6 and 10 are isolated from this plant for the first time.

Objective To investigate the characteristics of growth and angiogenesis of colon cancer xenograft in nude mice with metabolic syndrome induced by high fat diet.Methods Female BALB/C nude mice were fed with high fat diet (45.0％ from fat,HFD group) or common diet (13.8％ from fat,CD group) for 12 weeks (n=15,respectively).Colon cancer cell line SW480 was marked with green fluorescent protein (GFP) and subcutaneous xenograft model was established.The tumor growth was observed by the in vivo imaging system in small animal at the 4th week.By the end of the experiment,serum glucose and lipid level of the two groups were measured,visceral subcutaneous and visceral adipose tissue,liver and xenograft tumor were dissociated and weighted.The differences of proliferating cell nuclear antigen (PCNA) and CD31 expression in the tumors between groups were analyzed.The t-test or x2 test were performed for group comparison.Results Compared with CD group,the body weight,blood serum glucose level,triglyceride and cholesterol level,adipose content of subcutaneous and visceral of the HFD group significantly increased (t=2.91,4.12,4.43,3.92,3.77 and 4.02,all P＜0.05).Averagedaily energy intake of HFD group was significantly higher than that of CD group (t=2.34,P＜0.05).There was no significant difference between the two groups in liver weight (t=1.02,P＞0.05).However,by HE staining lipid vacuoles in the liver tissue was obvious in HFD group.Average bioluminescent index,tumor volume and weight of xenografts of HFD group were remarkably higher than those of CD group (t=8.84,2.48 and 2.86,all P＜0.05).The immunohistochemical staining results indicated that the strong positive rate of PCNA in xenografts of HFD group was 80.00％ and the microvessel density (MVD) was (25.75±0.96)/per high power field,both of which were higher than those of CD group (14.29％ and (13.33±1.53)/per high power field respectively,x2 =12.52,t=13.35,both P＜0.01).Conclusions The colon cancer xenograft in nude mice with metabolic syndrome induced by high fat diet had a high MVD and grew fast.

Objective: To observe the analgesic and anti-inflammatory effect of mandelic acid. Methods: Fifty Kunming mice were randomly divided into 5 groups:the blank control group (0. 1 ml/10 g), mandelic acid high (300 mg·kg-1), medium (200 mg ·kg-1 ) and low (140 mg·kg-1 ) dose groups, and the positive control ( aspirin) group, ig, qd. The analgesic effect of mandelic acid was observed by writhing test and hot plate method in mice. The ear swelling model caused by dimethyl benzene in mice was a-dopted to observe the analgesic effect. Results:Mandelic acid in each dose group could make the number of writhing in mice signifi-cantly reduced and pain threshold extended, and when compared with the blank control group, the difference was statistically significant (P<0. 01). The writhing times of mice mandelic acid high dose group was fewer than that of the positive control group, and there was no statistically significant between the groups (P>0. 05). In low and medium dose group, the writhing times of mice were more than those of the positive control group, and there was a significant difference between the low dose group and the positive control group( P<0. 05). The pain threshold of the mice in each mandelic acid dose group was higher than that of the positive control group, the pain threshold increased significantly in the high dose group before and after the administration, and the difference was statistically signifi-cant when compared with the positive control group (P<0. 05). The effect of mandelic acid on the ear swelling of mice was not signifi-cant, and when compared with the blank control group, the difference was not significant (P>0. 05). Conclusion:Mandelic acid has significant analgesic effect, while anti-inflammatory effect is not obvious.