In order to observe how Hachiko changes according to diseases or Sho, the adequacy of the eight parts of the body was examined in the 2200 cases who visited the Institute for Oriental Medicine in Nagoya. The Hachiko consisted of the eight parts of the human body divided into up and down, right and left, and front and back according to Yin-Yang dualism. Electrical skin resistance was measured at the terminal points using a Motoyama's measuring apparatus for the function of the meridians and the organs. Sho was determined by the diagnosis of oriental medicine. The skin resistance was applied to each of the eight parts and each average ratio of the 2200 cases was determined. The results showed the well-balanced distribution of each part's average ratio of the 2200 cases. Corresponding to the Yin-Yang theory, in which a well-balanced Yin-Yang condition is regarded as a normal physiological state, these results suggested the adequacy and importance of the Yin-Yang theory.

INTRODUCTION
The basic concept of oriental medicine is that of the dual phenomenon of Nature and living beings, a concept which has been rendered relative via philosophy. In particular, the fundamental idea, viewed dualistically, is that for ane one phenomenon there are two relative aspects, that of YIN and YANG.
METHODS
This time we used anatomical positions: left is yang and right is yin, upper is yang and lower is yin. We took the view that there is an analogy between the above and what Kentaro Takagi proved, namely pressure vis a vis the semi lateral sweating reflex. Between June 1976 and September 1979, using the six fixed position type pulse diagnosis we examined 1400 patients coming to the research lab (male, 744. female, 655). We also used the Motoyama type machine for meridian/organ function measurement, from which we obtained the electrical resistance value of well points (sei ketsu). Then we quadridemarcated the body into left and right together with hands and feet.
RESULTS
By comparing the 1400 cases and separating male/female for each diagnostic group, we classified these into quadridemarcation charts and observed the patterns. We came up with;
1) It was proved that a quadridemarcarcation pattern exists.
2) A left/right resistance and a lower/upper resistance was proved.
3) No difference was noted in any difference between male and female as cited in the classics.
4) Changes in the pulse vis a vis diagnosed patterns were noted.
5) The difference of each diagnosed group as well as the correspondence between pulse diagnosis and quadridemarcation of the body was proved. From the above results we would like to report that the basic yin yang concept of oriental medicine is of great importance as noted in the classics.

Pulse diagnosis has been taking an important part, together with the other diagnostic techniques, for determination of symptoms and treatment of patients with acupuncture. However, the traditional expression of pulse is complicated and subjective, so that the understanding of pulse diagnosis in termes of modern medicine is almost impossible. We tried to objectify the expression of pulse wave.
The pulse wave recorded from the six positions on radial artery according to the method described in Chinese Medicine through a force transducer. The recordings were processed for Fourier analysis utilizing a computer system. Characteristics of the pulse wave can be expressed quantitatively as a form of power spectrum of its components. The preliminary results of this study was reported at the 31th annual meeting of the Japan Society of Acupuncture. This time, we report the analysis on the basic pulse waves which have been recognized as negative-positive and imaginaryreal pulses.
Method
The subjects were normal volunteers of 36 males and 17. females. A force tranducer (Nihon Kohden TF-11S) Equipped with 0.52cm² of sensitive area was placed on the radial artery under the pressure ranging from 20 to 550gr. The pulse wave thus recorded was led into the electronic computer (HITAC-20) to obtain the power spectrum of the frequencies.
Results
It was found that characteristics of the six kinds of pulse waves, i. e., floating-sunken, smooth-leap and slow-fast types, can be represented quantitatively in terms of frequency histogram. Further, a possibility to express so called "complex pulse" in Chinese Medicine by this method is suggested.

We investgated the effect of electric acupuncture on peripheral T lymphocyte subset from normal individuals, analysed by laser flow cytometric system with monoclonal antibodies for human T lymphocyte subset.
OKT3⁺ cells (peripheral T cells) and OKT4⁺ cells (helper/inducer cells) did not show any changes after electric acupuncture. OKT8⁺ cells (suprressor/cytotoxic T cells), however, OKT11⁺ cells (E rosette positive T cells) and Leu7⁺ cells (natural killer cells) increased and Leu11⁺ cells (another population of natural killer cells) decreased after electric acupuncture.
These changes of peripheral T lymphocyte subsets would suggest that electric acupuncture effects human immune system.

In our clinic, blood pressure measurement has been done for many patients with various chief complaints. Acupuncture treatment was undertaken to improve their chief complaints according to Kurono's whole-body adjustment. We carried out a research paying attention to the fact that the patients with elevations of the blood pressure at the first visit showed a tendency to gradual decrease of the values.
According to the values of the first measurement, patients were divided into two groups in accordance with WHO's criteria: Hypertension group and Boundary one.
The first measurement velues and the averaged values of the following seven measurements undertaken before treatment were compared using histgram and t-test.
The descent of the blood pressure was seen in both Hypertension and Boundary group.

Although the efficacy of acupuncture in treatment of various diseases has been empirically established, systematic demonstration of this point has been rarely made. Our animal experiments designed to prove the efficacy of acupuncture on a scientific basis are reviewed here.
Each of the following experiments was carried out on two to five groups of mice. Mice in acupuncture group (A-animals) were acupunctured at four to seventeen points on every second or third day beginning with the first experimental day.
Exp. I. The preventive effect of acupuncture on acute poisoning by carbon tetrachloride-With carbon tetrachloride (4.16g/kg body weight) injected in the abdominal cavity on the 18th exp. day, all the non-acupunctured mice (N-animals) died in 72 hours after the administration, while 16% of the A-animals survived.
Exp. II. The curative effect of acupuncture on subacute liver injury induced by carbon tetrachloride. -Carbon tetrachloride (3.47g/kg b. w.) was administered on the 17th exp. day in the same manner as Exp. I. Levallorphan tartrate (antagonist to narcotics) was given to half of the A-animals (L+A-animals) and control animals (L-animals) before each session of acupuncture. Electron microscopic examination revealed severely injured liver cells in the N-animals and very little changes in the A-animals. The liver of L+A-animals was injured to some extent. Since the explanation of toxicity of levallorphan was refuted by the L-animals, this result suggests that some narcotic substances play a role in the process.
Exp. III. The effect of acupuncture on alloxan diabetes. -Alloxan (6mg) was administered on the 8th exp. day, and the animals were sacrificed on the 30th or 46th exp. day for examination. The N-animals showed extremely high blood sugar, though the A-animals did not. Ultrastructural study revealed that characteristic changes of alloxan diabetes in the pancreas had almost disappeared from the A-animals.
Exp. IV. The effect of acupuncture on renal injury induced by mercuric chloride. -With mercuric chloride (0.2mg/100g b. w.) administered three times a week, 10% of the N-animals and none of the A-animals died during the three-month period. Fairly heavy proteinuria (30-100mg/ml) was detected in the N-animals, though uria of A-animals was almost normal. After a one-month period, the N-animals showed a significant weight loss compared to the A-animals. Through the use of the electron microscope, severe injuries were seen in the epithelium of the renal tubules of the N-animals, and healed injuries in that of the A-animals.
Ultrastructural evidence has been presented that acupuncture is effective in (1) preventing and curing liver injury induced by carbon tetrachloride, (2) curing alloxan diabetes, (3) curing nephritis induced by mercuric chloride. Our hypothesis suggesting the possible mechanism that underlies these effects produced by acupuncture has been briefly explained.

We have twice previously reported the results of our preliminary investigation regarding patients with hypertension. Recently, the focus of our interest has been on the blood pressure levels at the time of the third sound of korotkoff. We reported our findings regarding this topic in an oral presentation titled “Evaluation of acupunctural treatment in hypertensive patients” at the 9th Annual Central Japan Block Meeting of the Japanese Society of Acupuncture and Moxibustion Therapy.
Since then, we have accumulated further clinical experience regarding hypertensive patients. In this study, we investigated the third sound of korotkoff in patients diagnosed as borderline hypertension according to the WHO criteria for hypertension. The blood pressure during the third sound of korotkoff ranged from 100mmHg to 110mmHg in our patients. This difference in blood pressure noted during the decreasing phase of the blood pressure cycle may serve as a useful prognostic indicator in hypertensive patients.