(1) Men who took a bath (of saline sodium bicarbonate hot spring) at 38°C for 15min. showed normalizations in the size, tonus and motion of their stomachs.
(2) By bathing in the above mentioned bath at 38°C for 30min. the amount of Mg²⁺, Cl^-, Ca²⁺ in the blood varied and that of inorganic phosphate in the blood increased.
(3) Rats bathed were either in city water at 39°C for 15min. or 30min., in saline sodium bicarbonate hot spring (Shirahama hot spring) at 39°C for 15min. or 30min., or in sodium bicarbonate hot spring (Ryujin hot spring) at 39°C for 15min. or 30min.
There were remarkable differences in the quantity of electrolytes and inorganic phosphate in hypodermic connective tissue according to the duration of bath and the quality of the hot springwaters.
(4) By taking a bath in tap water or hot spring water the amount of electrolytes and minerals both in the blood and in the hypodermic connective tissue varied. The degree of variation differed by the character of waters and by the duration of bath.
This variation of the quantity of electrolytes and minerals in the blood possibly caused the change in the shape, the tension, and the motion of the stomach, and then normalize.
By these experiments the anthor could explain a part of the effects of hot spring bath.

It was clarified that the effects of hot spring water bathing are not common to all the waters, but they differ by the quality of water.
Bathing in fresh hot spring water, which is said to be effective, increases and decreaess electrolyte in connetive tissue a great deal.
It seems to be one of the factors in the effects of hot spring water bathing.

Saline sodium bicarbonate hot spring bath (of Shirahama Spa). influences lipid metabolism.
But it made no notable change by single bath.
The increase and decreases of steroids and of fatty acids were observed by means of small amount measurement.
After serial bathings for about a month there were marked increases and decreases of serum lipid, total cholesterol, cholesterol ester, NEFA, & phospho-lipid.
It was observed that dissolution of fat in the body was induced and that hypercholesterolemia was cured by normalization and blood cholesterol was reduced nearly to normal.
Thus the author proved the efficacy of saline sodium bicarbonate hot spring bath from the study of lipid.

The author compared by TLC the increase and decrease of lipid, steroid, amine, and estrone in the hypodermic connective tissue with those in the liver caused by applying IR and UV to rats' skin.
In the connective tissue the variations were more remarkable than in the liver, and in some cases fatty acid and steroid were reduced notably, which was more prominently affected by IR irradiation than by UV.
On the other hand, in many cases they diminished in the liver, and, generally, they were more markedly influenced by UV.
In some instances the variations in the liver were reversed to those in the connective tissue.
It is thought this is caused by the metabolic relation between the liver and the connective tissue, or by the change of what the author assert regarding the connection between the hypodermic connective tissue and the internal organs by nucleic acid, mucopolysaccharide, ions, etc.

The effect of bathing with hot spring water did not originate from non-specific physical stimulation to the whole body, but each tissue, for examples, the bone marrow, subcutaneous connective tissue or liver, was influenced in a different manner. It was considered that there were close relation among these effects.

There is a difference between city water bathing and hot spring water bathing in changing lipid metabolism, steroid metabolism etc. in serum and connective tissue.
Remarkable differences are seen due to the quality of hot spring water and also due to the duration of bathing.
It is usually observed that fatty acid and steroid in serum increased and decreased contrary to those in connective tissue.
From the changes of lipid metabolism I studied the special function of hot spring bathing.