Through establishing QCC,the theme is confirmed,the plan is made,and the data is analyzed to explore QCC on improving the nursing unit of tumor patients'pain.(1)Visible result shows that,through the activities of QCC,the pain assessment accuracy is markedly increased from 70.48％ to 90.48％.The difference is statistically significant (P＜0.05).(2)Invisible result shows that QCC members mark themselves based on QCC grasp degree,team-work ability,professional knowledge,communication coordinate ability,confidence,responsibility and enthusiasm before and after QCC.

Objective To study the effect of Smad4 gene on angiogenesis related factors in human gastric cancer cell line.Methods Recombinant eukaryotie expressing plasmid pcDNA3.1 (-)-Smad4 containing Smad4 gene and empty vector pcDNA3.1 (-) were introduced into human gastric cancer cell line MKN28 using lipofectam and selected by G418,respectively.Two cell lines were obtained as follows:Smad4+-MKN28 cell line which was MKN28 transfected with a stable hybrid containing Smad4 gene and Smad4--MKN28 cell line with empty plasmid as control.The transcription and expression of VEGF and TSP1 were investigated by RT-PCR and Western blot.Results The mRNA expression of TSP1 in Smad4+-MKN28 cells was higher (P＜0.05) than that in control cells,while VEGF was lower(P＜0.05).Western blot showed the consistent results as measurement by RT-PCR.Conclusion Smad4 restoration in gastric cancer cells reduced angiogenesis rates through down-regulation of angiogenesis activitor and up-regulation of angiogenesis inhibitor.