2.Antitumor activities and its immunologic functions of Laurencia terpenoids
Hui LIANG ; Juan HE ; Shicui ZHANG ; Chunjing DONG ; Aigu MA
Chinese Journal of Marine Drugs 1994;0(01):-
Objective To investigate the antitumor and immunologic activities of Laurencia extract(LET) in Sarcoma 180(S 180).Methods The content of total terpenoids in LET was detected by RP-HPLC and its toxicity(LD 50) was estimated by Horn assay. Tumor inhibition rates, index of thymus and spleen, proliferation of spleen lymph cells, IgA,IgG,IgM were detected. The data were analyzed by SPSS software. Results The content of total terpenoids in LET was proved to be 63.29%. LET showed low toxicity with its LD 50 more than 3160mg?kg -1. Tumor inhibition rates of test groups were significantly higher than those of the control group. LET could obviously increase the levels of index of thymus and spleen. LET increase the multiplication of spleen lymph cell.Concentrations of IgA、IgG and IgM in plasma of the test groups were higher than those of the control group. Conclusion LET rich in terpenoids is safety to be taken orally. The alga extract showed obvious antitumor activities and immunologic functions.
3.Establishment of transgenic receptor system and aFGF transformation in astragalus.
Dachao LI ; Yi WANG ; Shicui JIANG ; Xiuwen HAN ; Meiping ZHANG
China Journal of Chinese Materia Medica 2009;34(19):2454-2457
OBJECTIVETo establish a high-frequency regeneration system of Astragalus and an aFGF transformation system.
METHODCotyledon node of the Astragalus explants was used for organogenesis to establish a high-frequency regeneration system. GV3101 was used to transform cotyledon node, and aFGF gene was introduced into Astragalus, renewable strain was detected by PCR.
RESULTAll cotyledon node was explants, adventitious buds were induced in the medium of MS +2.0 mg x L(-1) BA +0.5 mg x L(-1) IBA, the root was taken in the medium of 1/2MS +5.0 mg x L(-1) NAA to give a high frequency regeneration system. All cotyledon node was precultured in medium for 3 days and infected with Agrobacterium (A600 0.3) for 10 min and then cocultured for 2 days. The aFGF gene was confirmed to transform into genome of Astragalus.
CONCLUSIONA high-frequency regeneration system of Astragalus and an aFGF transformation system were established.
Astragalus Plant ; genetics ; metabolism ; Fibroblast Growth Factor 1 ; genetics ; metabolism ; Gene Expression Regulation ; Genetic Engineering ; methods ; Humans ; Plants, Genetically Modified ; genetics ; metabolism ; Transformation, Genetic
4.A rudimentary study of the acid fibroblast growth factor's plant expression vector construction and transformation tobacco.
Shicui JIANG ; Yi WANG ; Xiaokun LI ; Xiuwen HAN ; Meiping ZHANG
Journal of Biomedical Engineering 2010;27(1):126-131
Acid fibroblast growth factor (aFGF) has great potential in clinical application, but it is very expensive. In order to reduce the cost of production and to make full use of the merits integrated with plant bioreator, we have explored the aFGF in transgenic Tobacco expression. AFGF gene was inserted into plant expression vector pBI121; the acquired plants contained aFGF gene expression vector pBI121-TOAB-aF. Using Agrobacterium-mediated gene transformation of Tobacco and using transgenic Tobacco containing kanamycin and cephalosporin culture medium, we obtained kanamycin resistant transgenic Tobacco plants. PCR detection, RT-PCR detection and Western blot detection confirmed that foreign genes were successfully expressed in Tobacco. These data could serve as a theoretical foundation on which to use the plant bioreactor for production of aFGF.
Agrobacterium
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genetics
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Fibroblast Growth Factor 1
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biosynthesis
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genetics
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Genetic Vectors
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genetics
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Plants, Genetically Modified
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genetics
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metabolism
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Recombinant Proteins
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biosynthesis
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genetics
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Tobacco
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genetics
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metabolism