Enzyme-histochemical investigations on experimental incised injury of thirty rabbits fortiming of wound were reported.In peripheral areas of wounds,strong reaction of six kinds ofdehydratase(ACP.AKP,ATP.ANAE.?—GA,?—Gr).were demonstrated 1～2 hrs followingincision while no reaction of four kinds of dehydrogenase(LDH.SDH.NADH.?—GPDH)werefound.The activity of dehydratase are explained by the enhanced metabolism of fibrocytes and theaggregation of acute inflammatory cells.The author suggests that the positive enzyme histochemicalreactions indicate the vital reaction in the first two hours,and PTAH staining is helpful for thediagnosis of micro—exudation of fibrin in the early stage of injury

Timing of death is one of the important problems in forensic pathologic practice.Using histochemical methods,the authors observed the enzyme activity changes ofPPr,CCo,ACP,ANAE,LDH,SDH,G6PD,NADHD,G-6-Pase and ATPase inmice liver cells at 0,6,12,24,48,72 hours postmortem(HPM) at the environmen-tal temperature 4℃ and 20℃.The results showed that at 20℃,PPr activity dec-reased markedly at 6 HPM,LDH and ATPase activity decreased at 48 HPM anddisappeared at 72 HPM.The another seven enzymes still showed good enzyme acti-vity at 72 HPM.The authors supposed that the results are significant for timingof postmortem duration.

This Paper reports the histochemical changes occcured at the early acute cardial ischemic area in-duced experimentally by ligation of left coronary artery of 50 male Wistar rats. The following histo-chemical changes at the ischemical areas were observed: obvious decrease of glycogen of cardical is-chemical for 1h, of both NADHD and LDH activity of ischemical for 2h as well as CCo,CK and ATP activity of ischemical for 4h. The decrease of CCo,CK and ATP activity propagated to the whole is-chemical area at the time of ischemical for 8h. The activity of NADHD,LDH'CK and ATP can toler-ated the postmortem autolysis influence up to 24h after death.

A case of the Sick Sinus Syndrome which was based on the diagnosis ofautopsy is reported in this paper.It was found that a decreased number ofpacemaker cells with fibrosis,fatty infiltration and the thick endothelium ofcenter arteriole in sinus-atrial node.Otherwise the vacuolar degenerationcould be seen in the bundle of his.There were no fetal pathological changesto be seen in other organs.

Fourty wistar rats were used for experimental study of myocardial infarction (MI )induced by ligation of the left coronary artery. The results showed that the AF and HBFP stalng as well as Ao fluorescence were sensitive in demonstrating the early MI changes occuced 1 ~ 2 hour after coronary arteryligation. The TTC stamg method was reliable for demonstrating the MI margin. Both HBFP and AOfluorescence were under no influeence of the post-mortem autolysis, but the AF reaction were undeithe influence of the early post-mortem autolysis. Four hours were required for complete developemenof MI. The early stage of MI demonstrated by SEM was conslstant with that bv TEM. Both resuhwere influenced by the post-mortem autolysis.

Objective:To explore the effect of artificial intelligence teaching-picture system in training the bone marrow cell morphological reading ability of clinical medical students.Methods:A total of 110 five-year undergraduate students were divided into experimental group (artificial intelligence picture teaching method) and control group (traditional teaching method) in the bone marrow cell morphology reading ability training. On the basis of multimedia teaching, the experimental group was given the teaching by using the bone marrow cell morphology picture storage and transmission system for retrieval, learning and computer adaptive test. Then objective evaluation of image recognition ability and questionnaire were used to compare the teaching effect.Results:The image recognition ability was significantly better in the experimental group than in the control group [(89.6±5.7) vs. (81.4±4.9), P<0.01]. Furthermore, the experimental group showed more obvious advantages in cell morphology recognition [(74.7±4.0) vs. (68.7±4.9)] and diagnosis of hematological diseases [(14.9±3.0) vs. (12.9±2.4)] than the control group (both P<0.01). Questionnaire survey showed that the students expressed their affirmation and support for the artificial intelligence teaching-picture system in the bone marrow cell morphological reading ability training. Conclusion:The application of artificial intelligence teaching-picture system can greatly improve the teaching effect, mobilize students' learning enthusiasm and expand learning resources, which is worthy of further promotion and application.

The clinical research model based on evidence - based medicine (EBM) can effectively improve students' learning initiative, cultivate their abilities to obtain information and solve problems, and strengthen their scientific and innovative thinking in clinical research. Moreover, the EBM-based clinical research model can also help supervisors to update knowledge system and maintain strong clinical innovative thinking and research ability. The EBM - based clinical research model was applied in the cultivation of postgraduate students in hematology, and through topic selection, new technique application, ethical number acquisition, registration of clinical research number, case enrollment, and statistical analysis, postgraduates can master the methods and process of clinical research, which lays a solid foundation for future research.