Objective To investigate the prevalent status of hypertensionamong thelow insurance crowd in Shiyan urban districts.Methods According to the demands and diagnosis standard in 'China Hypertension Prevention and Cure Manual,2005',24 840 residents with low insurance in urban districts were examined on their blood pressure,while investigated on history of disease,family history,occupation and awareness on hypertension by face to face interview.Results Of 24 840 residents with low insurance in Shiyan urban districts,5 295 cases(21.30%) were diagnosed as hypertension,and 1,350 hypertensives had family history of hypertension.Of 5 295 hypertensives,there were 371 cases aged below 40 yrs,1 747 aged between 40 and 49 yrs and 3 177 aged above 50.Meanwhile,there were 3 105 cases with grade I hypertension,1 563 with grade II and 627 with grade Ⅲ hypertension.Conclusion The total hypertension prevalent rate among low insurance crowd of Shiyan urban districts was higher and the awareness rate was also lower.It should strengthen education on this crowd.

Objective To evaluate the diagnostic role of high frequency ultrasonography(HFU) and CDFI for breast tumor,especially to evaluate the correlation of pathology in hyperplasia of breast.Methods HFU and CDFI findings were compared with pathological study in 196 cases with confirmed breast tumor.The tumor of breast were divided into three groups.They were 81 cases of fibrocystic disease ofbreast,70 cases of breast cancer and 45 cases of fibroadenoma disease of breast.Results The benign and nicious tumor of breast haddifferent and typical represent of HFU,because dates of HUF had correlation with those of pathology,such as tumor boundary,internalecho,mononode and multinode,calcification,attenuation and CDFI.Some patients had crossed represent of HUF and CDFI.Thesensitivity,specificity and accuracy rate were calculated apart from.The sensitivity of fibroscystic disease of breast was 89%,the specificity was 96.5%,the accuracy was 93%,the negative predictive value was 93%,the postive predictive valus was 95%;the sensitivity of cancer of breast was 89%,the specificity was 94%,the accuracy was 92%,the negative predictive value was 96%,the post predictive valus was 89%;the sensitivity of fibroadenoma of breast was 89%,the specifity was 93%,the accuracy was 92%,the negative predictive value was 96%,the postive predictive value was 80%.Conclusion HUF in combination with CDFI are useful in diagnosis of both male and female breast tumor.This study may be helpful in raising the ultrasonic diagnosis rate in breast tumor.

Objective To explore the biomechanical mechanism of long-term complication of cervical spinal operations by measuring pressure changes of zygapophysial joints using pressure sensitive film (PSF) after cervical spine anterior operations. Methods C4,5 anterior discectomy and C4,5 anterior plate fixation were done on 10 fresh adult cervical spine specimens (C2～T1). PSF was put into zygapophysial joints and pressure changes were measured in positions of extension, left/right lateral bending and axial rotation on a universal testing machine. Results After cervical spine anterior operations, pressure of the adjacent facet joints increased. The differences were significant in extension and axial rotation (P

Objective To investigate the effects of brain-derived neurotrophic factor (BDNF) pretreatment on H9c2 myocardial hypoxia/reoxygenation (H/R) injury, and explore its mechanism. Methods The H9c2 myocardial cells were cul?tured in vitro and (95%O2+5%CO2) oxygen cultured 12 h after (95%N2+5%CO2) hypoxia cultured 4 h to establish the H/R model. The cells were divided into normal control group, H/R group, different concentrations (1, 10, 100μg/L) BDNF pre?treatment in H/R groups and TrkB-inhibitor group (with 100μg/L BDNF and 1∶1 000 TrkB inhibitor pre-treatment in H/R group). The cell survival rate was measured by MTT method in different groups. The lactate dehydrogenase (LDH), creatine kinase (CK), malondialdehyde (MDA) and superoxide dismutase (SOD) content and activity were detected after H/R injury. The apoptotic rate of H9c2 myocardial cells were detected by flow cytometry, and the expressions of TrkB, Bcl-2 and Bax protein were detected by Western blot assay. Results Compared with the normal control group, the survival rate of H9c2 myocardial cells was decreased significantly in H/R model group (P < 0.05), LDH, CK and MDA contents were increased and SOD activity was decreased (P<0.05). The cell apoptosis rate was increased significantly (P<0.05). The anti-apoptosis Bcl-2 protein expression was decreased, pro-apoptosis Bax protein expression was increased in H/R model group (P<0.05). Compared with the H/R model group, the cell survival rates of H9c2 myocardial cells were increased after pre-treatment with different concentrations of BDNF (P<0.05);LDH, CK and MDA contents were decreased and SOD activity were in?creased respectively (P < 0.05). The cell apoptotic rates were decreased (P < 0.05). The expressions of TrkB receptor and Bcl-2 protein gradually increased, while the expression of Bax protein was gradually decreased (P<0.05). The role of BDNF was inhibited by TrkB inhibitor. Conclusion BDNF pre-treatment can promote the cell survival rate of H9c2 myocardial cells after H/R injury, which plays a protective role by inhibiting the cell apoptotic rate and maintaining antioxidant capacity, and associates with BDNF-TrkB signaling pathways.

Objective: To investigate the effect of tanshinone IIA (TSN) on left ventricular hypertrophy (LVH) and cardiomyocyte apoptosis in spontaneous hypertensive rats (SHRs). Methods: A total of 60 SHRs at 8 weeks of age were randomly divided into 3 group: Blank control group, the rats were sacriifced at 8 weeks, TSN group, the rats were treated with TSN at 1 ml/(kg?d) for 18 weeks and Solvent control group, the rats were treated with the solvent at 1 ml/(kg?d) for 18 weeks. n=20 in each group and 15 rats were used for the experiments. The systolic blood pressure (SBP) and left ventricular mass index (LVMI) were examined, cardiomyocyte’s diameter and surface area were measured by HE staining, the apoptosis rate was evaluated by TUNEL method and the apoptosis related protein expression s of Bcl-2, Bax and p53 were determined by Western blot analysis. Results: ①Compared with Solvent control group, TSN group had decreased LVMI (3.23 ± 0.24) mg/g vs (4.58 ± 0.68) mg/g,cardiomyocyte’s diameter (16.13 ± 1.77) μm vs (27.15 ± 3.52) μm and surface area (230.23 ± 69.37) μm2 vs (490.12 ± 118.96) μm2and decreased apoptosis rate (7.45 ± 1.78) % vs (10.61 ± 2.77) %, allP<0.01.②With NAPDH reference correction, compared with Solvent control group, TSN group presented increased protein expression of Bcl-2 (0.97 ± 0.31) vs (0.40 ± 0.11) and decreased Bax (0.37 ± 0.15) vs (1.81 ± 0.44), decreased p53 (0.83 ± 0.18) vs (2.72 ± 0.28), allP<0.05 or P<0.01. The above indexes were similar between TSN group and Blank control group,P>0.05. Conclusion: TSN could inhibit the development of LVH and decrease the cardiomyocyte apoptosis, which might be via up-regulating the protein expressions of Bcl-2 and down-regulating Bax and p53 in SHRs.

[ABSTRACT]AIM:Tostudytheprotectiveeffectofbrain-derivedneurotrophicfactor(BDNF)onvascularendo-thelial cells with H 2 O2-induced oxidative injury .METHODS: Human umbilical vein endothelial cells ( HUVECs ) were cultured in vitro, and the oxidation injury model of HUVECs was established by treatment with H 2 O2 .The oxidatively in-jured HUVECs were cultured with different concentrations (1, 10 and 100μg/L) of BDNF.At the same time, the control group (no injury), PBS treatment after H2O2 injury group and TrkB inhibitor group (with 100 μg/L BDNF and 1∶1 000 TrkB inhibitor) were also set up.The viability of the HUVECs was detected by MTT assay .The levels of LDH, MDA, SOD and GSH were measured .The releases of NO , ET-1 and ICAM-1 were analyzed by ELISA .The changes of ROS pro-duction and cell apoptosis were evaluated by flow cytometry .The protein levels of TrkB , p-TrkB, cleaved caspase-3, Bcl-2 and Bax were determined by Western blot .RESULTS:Compared with uninjured control group , in H2 O2 oxidative injury plus PBS treatment group , the viability of the cells was decreased significantly , the LDH and MDA levels were increased significantly and the activities of SOD and GSH were decreased significantly .The NO secretion was decreased , and the ET-1 and ICAM-1 concentrations were increased significantly .The ROS content and apoptotic rate were increased significantly . The protein levels of cleaved caspase-3 and Bax were increased but Bcl-2 protein expression was decreased significantly . Compared with PBS treatment group , in H2 O2-injured HUVECs treated with different concentrations of BDNF , the cell via-bility was gradually increased , the LDH and MDA levels were decreased and the activities of SOD and GSH were increased gradually .The secretion of NO was increased but ET-1 and ICAM-1 were decreased gradually .The ROS content and apop-totic rate were decreased significantly .The TrkB and p-TrkB levels were significantly increased significantly , the protein expression of cleaved-caspase 3 and Bax was decreased gradually and the Bcl-2 protein expression increased gradually .The role of BDNF was inhibited by TrkB inhibitor .CONCLUSION:BDNF protects HUVECs from oxidative injury by binding with TrkB to activate the BDNF-TrkB signaling pathways .

Objective: To explore the value of TACE combined with interventional embolization of hepatic arterio-portal fistula (HAPF) in treatment of primary hepatocellular carcinoma (PHC). Methods: Clinical data of 65 advanced PHC patients with HAPF were retrospectively analyzed. According to the time of portal vein appearance during hepatic artery angiography, there were 31 patients with low flow type HAPF (<1 s), 25 with medium flow type (1-3 s) and 9 with high flow type HAPF (>3 s). The patients were treated with TACE combined with embolization of the fistula. Then clinical efficacy and closure of fistula were evaluated 1 month after treatment, and the postoperative survival rates of 3, 6, 12 and 24 months were counted. Results: HAPF were successfully one-time embolized in 48 cases (73.85%, 48/65), including 26 cases (26/31, 83.87%) of low-flow type, 18 cases (18/25, 72.00%) with medium-flow type and 4 cases (4/9, 44.44%) with high-flow type HAPF (P=0.046). One month after treatment, tumor progression was observed in 11 patients, while stable in 19 and remission in 35 patients, respectively. Tumor progression, stable and remission was found in 2, 3 and 26 cases among patients with low-flow orifice fistula, 2, 14 and 9 cases among those with medium-flow type HAPF, 7, 2 and 0 case among high-flow type HAPF, respectively (P=0.001). The survival rate was 87.69% (57/65) 3 months, 67.69% (44/65) 6 months, 43.08% (28/65) 12 months and 6.15% (4/65) 24 months after treatment. The difference of survival rate among patients with low-, medium- and high-flow type HAPF was statistically significant (P<0.001). Conclusion: TACE combined with interventional embolization of HAPF has good therapeutic effect, especially for PHC patients with low-flow type HAPF.

Objective To evaluate the efficacy of modified arytenoid adduction in the management of patients with unilateral vocal fold paralysis(UVFP).Methods A retrospective review was performed on 22 patients who underwent modified arytenoid adduction for UVFP between February 2001 and December 2007.Pre-,and 3 months postoperative aspiration,perceptual(GRBAS),acoustic data(fundamental frequency,F_0,fundamental frequency perturbation,jitter,amplitude perturbation,shimmer,normalized noise energy,NNE)and aerodynamic(maximal phonatory time,MPT,mean airflow rate,MFR)were analyzed statistically.Results The ratings of postoperative aspiration were significantly decreased than that of the preoperation(P<0.0001).There was a significant decrease in GRBAS scales postoperatively versus preoperatively(P<0.0001).The mean values of voice acoustics parameters (F_0,jitter,shimmer,NNE)were significantly decreased,the maximum phonation time were significantly longer,and the mean airflow rate were significantly decreased after operation than that of the preoperation(P<0.001).Conclusion Modified arytenoid adduction is an effective medialization technique that can restore satisfactory speech and prevent aspiration in patients with UVFP.

Objective To design a new Chinese mandarin version of speech sample text for perceptual assessment .Meth-ods The speech sample text included all phonemes and limit number of words .The correlation analysis of the constituent rati-os of all phonemes was performed between the speech text and the ones in Chinese language reported by the institute of Acous-tics Chinese Academy of Sciences using Pearson's correlation coefficient and Spearman correlation coefficient .And their differ-ences were tested by paired sample t test and Wilcoxon signed rank test .Legibility was alsotested .Results Our speech sarrple text contained 21 vowel ,38 consonant and 4 tones .The proportions of vowel ,consonant and tone in the speech text had high correlation with those in Chinese language (for proportions of vowel ,the Pearson's correlation coefficient was 0 .908 and Spearman correlation coefficient was 0 .775 ,P<0 .01 ,respectively ;for proportions of consonant ,the Pearson's correlation co-efficient was 0 .802 and Spearman correlation coefficient was 0 .808 ,respectively ,P<0 .01 ;for proportions of tone ,the Pear-son's correlation coefficient was 0 .967 and Spearman correlation coefficient was more than 0 .999 ,respectively , P< 0 .01) . There was no difference in the proportions of vowel ,consonant and tone between them(P>0 .2) .The value of legibility was 2 , indicating legibility of the speech sample text was good .Conclusion The new Chinese mandarin version of speech sample text can be used in perceptual assessment .The value for perceptual assessment still needs further clinical research .

To isolate human phage single chain antibody against surface protein of Streptococcus mutans,the recombinant surface protein of S.mutans(rAP) was used to coat the immune tubes and the phage single chain antibody was prepared through pDAN5 phage antibody library after 5 rounds of panning.The eluted phage was enriched nearly 30 times.In these ways,13 positive clones were obtained and found to be able to bind with rAP in ELISA assay.Then one of the 13 positive clone phage plasmid was used to infect E.coli HB2151 to induce the expression of the non-fusion single chain antibody (ScFv) with IPTG induction.As demonstrated by SDS-PAGE,the molecular mass of this single chain antibody was proved to be 30 kDa and the amount of expression constituted to 30% of the total bacterial proteins.Apparently,the human phage single chain antibody against surface protein of S.mutans with biological activity was successfully screened.